ID   KLDC3_RAT               Reviewed;         382 AA.
AC   Q6AYI2;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2004, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Kelch domain-containing protein 3 {ECO:0000250|UniProtKB:Q9BQ90};
GN   Name=Klhdc3 {ECO:0000312|RGD:1307105};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Substrate-recognition component of a Cul2-RING (CRL2) E3
CC       ubiquitin-protein ligase complex of the DesCEND (destruction via C-end
CC       degrons) pathway, which recognizes a C-degron located at the extreme C
CC       terminus of target proteins, leading to their ubiquitination and
CC       degradation. The C-degron recognized by the DesCEND pathway is usually
CC       a motif of less than ten residues and can be present in full-length
CC       proteins, truncated proteins or proteolytically cleaved forms. The
CC       CRL2(KLHDC3) complex specifically recognizes proteins with a glycine
CC       (Gly) at the C-terminus, leading to their ubiquitination and
CC       degradation: recognizes the C-terminal -Arg-(Xaa)n-Arg-Gly, -Arg-
CC       (Xaa)n-Lys-Gly, and -Arg-(Xaa)n-Gln-Gly degrons. The CRL2(KLHDC3)
CC       complex mediates ubiquitination and degradation of truncated SELENOV
CC       and SEPHS2 selenoproteins produced by failed UGA/Sec decoding, which
CC       end with a glycine. May be involved in meiotic recombination process.
CC       {ECO:0000250|UniProtKB:Q9BQ90}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000250|UniProtKB:Q9BQ90}.
CC   -!- SUBUNIT: Component of a CRL2 E3 ubiquitin-protein ligase complex, also
CC       named ECS (Elongin BC-CUL2/5-SOCS-box protein) complex, composed of
CC       CUL2, Elongin BC (ELOB and ELOC), RBX1 and substrate-specific adapter
CC       KLHDC3. {ECO:0000250|UniProtKB:Q9BQ90}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BQ90}.
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DR   EMBL; BC079035; AAH79035.1; -; mRNA.
DR   RefSeq; NP_001012203.1; NM_001012203.1.
DR   RefSeq; XP_006244604.1; XM_006244542.3.
DR   AlphaFoldDB; Q6AYI2; -.
DR   SMR; Q6AYI2; -.
DR   STRING; 10116.ENSRNOP00000023979; -.
DR   PaxDb; Q6AYI2; -.
DR   Ensembl; ENSRNOT00000023979; ENSRNOP00000023979; ENSRNOG00000017495.
DR   GeneID; 363192; -.
DR   KEGG; rno:363192; -.
DR   UCSC; RGD:1307105; rat.
DR   CTD; 116138; -.
DR   RGD; 1307105; Klhdc3.
DR   eggNOG; KOG4693; Eukaryota.
DR   GeneTree; ENSGT00940000157952; -.
DR   HOGENOM; CLU_045453_0_0_1; -.
DR   InParanoid; Q6AYI2; -.
DR   OMA; AYEQERM; -.
DR   OrthoDB; 933937at2759; -.
DR   PhylomeDB; Q6AYI2; -.
DR   TreeFam; TF354289; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q6AYI2; -.
DR   Proteomes; UP000002494; Chromosome 9.
DR   Bgee; ENSRNOG00000017495; Expressed in testis and 19 other tissues.
DR   Genevisible; Q6AYI2; RN.
DR   GO; GO:0000785; C:chromatin; ISO:RGD.
DR   GO; GO:0031462; C:Cul2-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR   GO; GO:0003682; F:chromatin binding; ISO:RGD.
DR   GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; ISS:UniProtKB.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   GO; GO:0140627; P:ubiquitin-dependent protein catabolic process via the C-end degron rule pathway; ISO:RGD.
DR   Gene3D; 2.120.10.80; -; 2.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR011498; Kelch_2.
DR   Pfam; PF01344; Kelch_1; 1.
DR   Pfam; PF07646; Kelch_2; 1.
DR   SUPFAM; SSF117281; SSF117281; 2.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Kelch repeat; Meiosis; Reference proteome; Repeat;
KW   Ubl conjugation pathway.
FT   CHAIN           1..382
FT                   /note="Kelch domain-containing protein 3"
FT                   /id="PRO_0000228997"
FT   REPEAT          25..77
FT                   /note="Kelch 1"
FT   REPEAT          88..138
FT                   /note="Kelch 2"
FT   REPEAT          139..189
FT                   /note="Kelch 3"
FT   REPEAT          191..249
FT                   /note="Kelch 4"
FT   REPEAT          251..301
FT                   /note="Kelch 5"
SQ   SEQUENCE   382 AA;  43109 MW;  37BC9835F7919B03 CRC64;
     MLRWTVHLEG GPRRVNHAAV AVGHRVYSFG GYCSGEDYET LRQIDVHIFN AVSLRWTKLP
     PVRPAVRGQA PVVPYMRYGH STVLIDDTVF LWGGRNDTEG ACNVLYAFDV NTHKWSTPRV
     SGTVPGARDG HSACVLGKIM YIFGGYEQLA DCFSNDIHKL DTSTMTWTLV CTKGNPARWR
     DFHSATMLGN HMYVFGGRAD RFGPFHSNNE IYCNRIRVFD TRTEAWLDCP HTPVLPEGRR
     SHSAFGYNGE LYIFGGYNAR LNRHFHDLWK FNPGSFTWKK IEPKGKGPCP RRRQCCCIVG
     DKIVLFGGTS PSPEEGLGDE FDLIDHSDLH ILDFSPSLKT LCKLAVIQYN LDQSCLPHDI
     RWELNAMTTN SNISRPIVSS HG