KLE2_CAEEL
ID KLE2_CAEEL Reviewed; 821 AA.
AC P34341;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2002, sequence version 2.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Condensin-2 complex subunit kle-2 {ECO:0000305};
DE AltName: Full=Kleisin, abnormal closure, protein 2;
GN Name=kle-2; ORFNames=C29E4.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=16094371; DOI=10.1038/nature03876;
RA Gunsalus K.C., Ge H., Schetter A.J., Goldberg D.S., Han J.D., Hao T.,
RA Berriz G.F., Bertin N., Huang J., Chuang L.S., Li N., Mani R., Hyman A.A.,
RA Sonnichsen B., Echeverri C.J., Roth F.P., Vidal M., Piano F.;
RT "Predictive models of molecular machines involved in Caenorhabditis elegans
RT early embryogenesis.";
RL Nature 436:861-865(2005).
RN [4]
RP FUNCTION, IDENTIFICATION IN A CONDENSIN II COMPLEX, INTERACTION WITH MIX-1;
RP SMC-4; HCP-6 AND CAPG-2, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=19119011; DOI=10.1016/j.cub.2008.12.006;
RA Csankovszki G., Collette K., Spahl K., Carey J., Snyder M., Petty E.,
RA Patel U., Tabuchi T., Liu H., McLeod I., Thompson J., Sarkeshik A.,
RA Sarkesik A., Yates J., Meyer B.J., Hagstrom K.;
RT "Three distinct condensin complexes control C. elegans chromosome
RT dynamics.";
RL Curr. Biol. 19:9-19(2009).
RN [5]
RP INTERACTION WITH MIX-1; SMC-4; CAPG-2 AND HCP-6, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=28301465; DOI=10.1371/journal.pgen.1006614;
RA Chao L.F., Singh M., Thompson J., Yates J.R. III, Hagstrom K.A.;
RT "An SMC-like protein binds and regulates Caenorhabditis elegans
RT condensins.";
RL PLoS Genet. 13:E1006614-E1006614(2017).
CC -!- FUNCTION: Regulatory subunit of the condensin II complex, a complex
CC that seems to play a role in prophase chromosome condensation and in
CC chromosome segregation in mitosis and in meiosis.
CC {ECO:0000269|PubMed:19119011}.
CC -!- SUBUNIT: Component of the condensin II complex, which contains the mix-
CC 1/SMC2 and smc-4/SMC4 heterodimer, and three non SMC subunits, capg-2,
CC kle-2 and hcp-6 that probably regulate the complex (PubMed:19119011).
CC Within the complex, interacts with mix-1, smc-4, capg-2 and hcp-6
CC (PubMed:19119011, PubMed:28301465). {ECO:0000269|PubMed:19119011,
CC ECO:0000269|PubMed:28301465}.
CC -!- INTERACTION:
CC P34341; Q20818: capg-2; NbExp=4; IntAct=EBI-2002861, EBI-6458495;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16094371,
CC ECO:0000269|PubMed:19119011}. Chromosome {ECO:0000269|PubMed:16094371,
CC ECO:0000269|PubMed:19119011}. Chromosome, centromere
CC {ECO:0000269|PubMed:16094371, ECO:0000269|PubMed:19119011}.
CC Note=Localizes to the core of each sister chromatid in meiosis
CC (PubMed:19119011). {ECO:0000269|PubMed:16094371,
CC ECO:0000269|PubMed:19119011}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown leads to disorganized
CC chromosome morphology, causes DNA bridges in mitosis and leads to
CC chromosome segregation defects in mitosis and meiosis.
CC {ECO:0000269|PubMed:19119011}.
CC -!- SIMILARITY: Belongs to the CND2 H2 (condensin-2 subunit 2) family.
CC {ECO:0000305}.
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DR EMBL; FO080706; CCD66003.1; -; Genomic_DNA.
DR RefSeq; NP_498727.2; NM_066326.6.
DR AlphaFoldDB; P34341; -.
DR BioGRID; 41323; 18.
DR ComplexPortal; CPX-1272; Condensin II complex.
DR IntAct; P34341; 13.
DR STRING; 6239.C29E4.2; -.
DR EPD; P34341; -.
DR PaxDb; P34341; -.
DR PeptideAtlas; P34341; -.
DR PRIDE; P34341; -.
DR EnsemblMetazoa; C29E4.2.1; C29E4.2.1; WBGene00016202.
DR GeneID; 176116; -.
DR KEGG; cel:CELE_C29E4.2; -.
DR UCSC; C29E4.2; c. elegans.
DR CTD; 176116; -.
DR WormBase; C29E4.2; CE30620; WBGene00016202; kle-2.
DR eggNOG; KOG2359; Eukaryota.
DR GeneTree; ENSGT00390000014443; -.
DR HOGENOM; CLU_013262_0_0_1; -.
DR InParanoid; P34341; -.
DR OMA; QMAYEMA; -.
DR OrthoDB; 551989at2759; -.
DR Reactome; R-CEL-2299718; Condensation of Prophase Chromosomes.
DR PRO; PR:P34341; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00016202; Expressed in embryo and 4 other tissues.
DR GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0000796; C:condensin complex; IPI:ComplexPortal.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0010032; P:meiotic chromosome condensation; IBA:GO_Central.
DR GO; GO:0045132; P:meiotic chromosome segregation; IC:ComplexPortal.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:WormBase.
DR GO; GO:0051306; P:mitotic sister chromatid separation; IBA:GO_Central.
DR InterPro; IPR031737; CNDH2_C.
DR InterPro; IPR009378; H2_N.
DR InterPro; IPR031739; Ncaph2.
DR PANTHER; PTHR14324; PTHR14324; 1.
DR Pfam; PF16858; CNDH2_C; 1.
DR Pfam; PF06278; CNDH2_N; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Meiosis;
KW Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..821
FT /note="Condensin-2 complex subunit kle-2"
FT /id="PRO_0000065201"
FT REGION 389..426
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 529..561
FT /evidence="ECO:0000255"
SQ SEQUENCE 821 AA; 94492 MW; 745D42E165340D53 CRC64;
MTRNAPPGQE STDLAWLVTP AKDLVENFSI DVLKALAGYL EVIRQESEDT DNQVDAATTY
RLFDFQRACR IIQGSCAVYG RKVDHVYELT ISVVDLVENK GQDDGNTGSR RGAGRRKNFN
LGSTNYDLAD IDSLKQEALA NFEKTVKEEK KSIDAVRMVE NAEVIESQYE RKSCLVAKPT
QFMFKLNYGQ LNRTDEQILN AKSRPDVIGK VKDFEIKKSK VKHDQQILYS HDCYRGNLDQ
FTLPGARWMP DNKELAANFG VADLEVELDL EQEHEKISAY GPFKDPLSGR EVVPPPRWFI
EQEAVRQNQE IQSRATSRAI TIAAKTLRDS QGFGSQPTRL SQPFVERHRQ SNHLNDFLSF
VEGRVNKNRP STHLTTGLVD MFVDNFGSVM QNDEPNTSRR PDENYAPMDF DDDFGGGGDD
DDDDYIRNLS RRDEKRAPAP WDELDKNHII WYTGDENLPV VSKPVKKITK FQPKPAEMLA
RKQRREEKIN KSRRDEFMET HDYLQDYYYW RSAARINPIK DWKIESLRTA ILAEKKRRIK
EKTAKIREAR IQNMQRKRTA RVIPVEQFEP VTEDIPTSNR RTLGAEYDDV VDEDLAAEVE
LSMFGGGFDD DEEDVRPRGE RPPMAPNNLE FDALQTDFDI PPAEYVPLRF EDIDDAELNS
VINLPGNLLI DKALPLLKKF AENRTDREQM AYEMAKAYED VDVAVSTLQE HVDKWHSRME
PILEEGETRK EYDVHAVGRA VIGQYDIEGG TKRLLDLVMD RPWYEISRYF LSCLFMCNVG
NVMVSEDMEL PLEERINSMK ITLLKRDMHC EMFKEAGALD A
