ARAE_ECO57
ID ARAE_ECO57 Reviewed; 472 AA.
AC P0AE25; P09830; Q46937;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Arabinose-proton symporter {ECO:0000250|UniProtKB:P0AE24};
DE AltName: Full=Arabinose transporter {ECO:0000250|UniProtKB:P0AE24};
GN Name=araE; OrderedLocusNames=Z4161, ECs3698;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Uptake of L-arabinose across the cytoplasmic membrane with
CC the concomitant transport of protons into the cell (symport system).
CC {ECO:0000250|UniProtKB:P0AE24}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + L-arabinose(in) = H(+)(out) + L-arabinose(out);
CC Xref=Rhea:RHEA:28951, ChEBI:CHEBI:15378, ChEBI:CHEBI:17535;
CC Evidence={ECO:0000250|UniProtKB:P0AE24};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28953;
CC Evidence={ECO:0000250|UniProtKB:P0AE24};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P0AE24}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
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DR EMBL; AE005174; AAG57953.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB37121.1; -; Genomic_DNA.
DR PIR; B91091; B91091.
DR PIR; E85936; E85936.
DR RefSeq; NP_311725.1; NC_002695.1.
DR RefSeq; WP_000256438.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0AE25; -.
DR SMR; P0AE25; -.
DR STRING; 155864.EDL933_4022; -.
DR EnsemblBacteria; AAG57953; AAG57953; Z4161.
DR EnsemblBacteria; BAB37121; BAB37121; ECs_3698.
DR GeneID; 66673292; -.
DR GeneID; 916479; -.
DR KEGG; ece:Z4161; -.
DR KEGG; ecs:ECs_3698; -.
DR PATRIC; fig|386585.9.peg.3865; -.
DR eggNOG; COG2814; Bacteria.
DR HOGENOM; CLU_001265_30_5_6; -.
DR OMA; WAITASF; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00879; SP; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Sugar transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..472
FT /note="Arabinose-proton symporter"
FT /id="PRO_0000050290"
FT TOPO_DOM 1..29
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 51..63
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 64..84
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 85..91
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 113..114
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..178
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..257
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 279..297
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 319..325
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 347..361
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 383..404
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 447..472
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AE24"
SQ SEQUENCE 472 AA; 51684 MW; 411990A441D44393 CRC64;
MVTINTESAL TPRSLRDTRR MNMFVSVAAA VAGLLFGLDI GVIAGALPFI TDHFVLTSRL
QEWVVSSMML GAAIGALFNG WLSFRLGRKY SLMAGAILFV LGSIGSAFAT SVEMLIAARV
VLGIAVGIAS YTAPLYLSEM ASENVRGKMI SMYQLMVTLG IVLAFLSDTA FSYSGNWRAM
LGVLALPAVL LIILVVFLPN SPRWLAEKGR HIEAEEVLRM LRDTSEKARE ELNEIRESLK
LKQGGWALFK INRNVRRAVF LGMLLQAMQQ FTGMNIIMYY APRIFKMAGF TTTEQQMIAT
LVVGLTFMFA TFIAVFTVDK AGRKPALKIG FSVMALGTLV LGYCLMQFDN GTASSGLSWL
SVGMTMMCIA GYAMSAAPVV WILCSEIQPL KCRDFGITCS TTTNWVSNMI IGATFLTLLD
SIGAAGTFWL YTALNIAFVG ITFWLIPETK NVTLEHIERK LMAGEKLRNI GV
