KLF1_DANRE
ID KLF1_DANRE Reviewed; 365 AA.
AC Q90XE6;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Kruppel-like factor 1 {ECO:0000303|PubMed:11535513};
DE AltName: Full=Erythroid krueppel-like transcription factor {ECO:0000312|ZFIN:ZDB-GENE-980526-55};
DE AltName: Full=Kruppel-like factor d {ECO:0000312|ZFIN:ZDB-GENE-980526-55};
GN Name=klf1 {ECO:0000312|ZFIN:ZDB-GENE-980526-55};
GN Synonyms=eklf {ECO:0000303|PubMed:33833125},
GN klfd {ECO:0000312|EMBL:AAK76995.1};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Proteomes:UP000000437};
RN [1] {ECO:0000312|EMBL:AAK76995.1}
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Kidney {ECO:0000312|EMBL:AAK76995.1};
RX PubMed=11535513; DOI=10.1182/blood.v98.6.1792;
RA Oates A.C., Pratt S.J., Vail B., Yan Y.L., Ho R.K., Johnson S.L.,
RA Postlethwait J.H., Zon L.I.;
RT "The zebrafish klf gene family.";
RL Blood 98:1792-1801(2001).
RN [2] {ECO:0000312|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000312|Proteomes:UP000000437};
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3] {ECO:0000312|EMBL:AAI62384.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND REPRESSION BY
RP MIR-144.
RX PubMed=18941117; DOI=10.1182/blood-2008-08-174854;
RA Fu Y.F., Du T.T., Dong M., Zhu K.Y., Jing C.B., Zhang Y., Wang L.,
RA Fan H.B., Chen Y., Jin Y., Yue G.P., Chen S.J., Chen Z., Huang Q.H.,
RA Jing Q., Deng M., Liu T.X.;
RT "Mir-144 selectively regulates embryonic alpha-hemoglobin synthesis during
RT primitive erythropoiesis.";
RL Blood 113:1340-1349(2009).
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY
RP INJURY.
RX PubMed=33833125; DOI=10.1126/science.abe2762;
RA Ogawa M., Geng F.S., Humphreys D.T., Kristianto E., Sheng D.Z., Hui S.P.,
RA Zhang Y., Sugimoto K., Nakayama M., Zheng D., Hesselson D., Hodson M.P.,
RA Bogdanovic O., Kikuchi K.;
RT "Krueppel-like factor 1 is a core cardiomyogenic trigger in zebrafish.";
RL Science 372:201-205(2021).
CC -!- FUNCTION: Transcription regulator (PubMed:18941117, PubMed:33833125).
CC Plays a role in erythropoiesis, binding to promoters and activating
CC transcription of embryonic alpha globin e1 hbae1.1 and microRNA mir-
CC 144, in erythroid progenitors; perhaps acting as part of a negative
CC feedback loop with mir-144 (PubMed:33833125, PubMed:18941117). May
CC coordinate repression of genes controlling myocardial differentiation
CC and mitochondrial metabolism with positive modulation of cell
CC proliferation genes (PubMed:33833125). Required for regeneration of the
CC myocardium after injury, probably acting by stimulating renewal of
CC preexisting cardiomyocytes in adult hearts (PubMed:33833125).
CC {ECO:0000269|PubMed:18941117, ECO:0000269|PubMed:33833125}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:33833125}.
CC Nucleus {ECO:0000305|PubMed:18941117, ECO:0000305|PubMed:33833125}.
CC Note=Colocalizes with the cytosolic muscle marker troponin C.
CC {ECO:0000269|PubMed:33833125}.
CC -!- TISSUE SPECIFICITY: Expressed in cardiomyocytes.
CC {ECO:0000269|PubMed:33833125}.
CC -!- DEVELOPMENTAL STAGE: First expressed in the inner cell mass (ICM) of
CC embryos at 18 hpf (hours post fertilization) and continues to be
CC expressed in the developing embryo until at least 48 hpf
CC (PubMed:18941117). Expressed in the developing hematopoietic system, at
CC 24 hpf and circulating primitive erythrocytes at 48 hpf
CC (PubMed:11535513, PubMed:18941117). Expressed in the pronephros, and in
CC circulating definitive erythrocytes in the heart lumen and vessels of
CC the tail at 8 dpf (days post fertilization) (PubMed:11535513).
CC {ECO:0000269|PubMed:11535513, ECO:0000269|PubMed:18941117}.
CC -!- INDUCTION: In adult heart myocardium upon injury (PubMed:33833125).
CC Repressed in embryos by microRNA miR-144 (PubMed:18941117).
CC {ECO:0000269|PubMed:18941117, ECO:0000269|PubMed:33833125}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown causes significant reduction
CC in expression of embryonic alpha globin e1 hbae1.1 in 22 hpf (hours
CC post fertilization) and 36 hpf embryos. {ECO:0000269|PubMed:18941117}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; BX601645; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC162384; AAI62384.1; -; mRNA.
DR EMBL; BC162393; AAI62393.1; -; mRNA.
DR EMBL; AF392996; AAK76995.1; -; mRNA.
DR RefSeq; NP_571011.1; NM_130936.1.
DR AlphaFoldDB; Q90XE6; -.
DR SMR; Q90XE6; -.
DR STRING; 7955.ENSDARP00000020778; -.
DR PaxDb; Q90XE6; -.
DR Ensembl; ENSDART00000011724; ENSDARP00000020778; ENSDARG00000017400.
DR GeneID; 30104; -.
DR KEGG; dre:30104; -.
DR CTD; 10661; -.
DR ZFIN; ZDB-GENE-980526-55; klf1.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000163957; -.
DR HOGENOM; CLU_067113_0_0_1; -.
DR InParanoid; Q90XE6; -.
DR OMA; ACWDMEL; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q90XE6; -.
DR TreeFam; TF350556; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 6.
DR Bgee; ENSDARG00000017400; Expressed in cardiac ventricle and 13 other tissues.
DR GO; GO:0000785; C:chromatin; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IC:ZFIN.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:ZFIN.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IMP:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IMP:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:ZFIN.
DR GO; GO:0031100; P:animal organ regeneration; IMP:UniProtKB.
DR GO; GO:0055007; P:cardiac muscle cell differentiation; IMP:UniProtKB.
DR GO; GO:0060038; P:cardiac muscle cell proliferation; IMP:UniProtKB.
DR GO; GO:0043697; P:cell dedifferentiation; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IDA:ZFIN.
DR GO; GO:0060319; P:primitive erythrocyte differentiation; IEP:ZFIN.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0045214; P:sarcomere organization; IMP:UniProtKB.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW Activator; Cytoplasm; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..365
FT /note="Kruppel-like factor 1"
FT /id="PRO_0000453283"
FT ZN_FING 282..306
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 312..336
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 342..364
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 243..264
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 243..258
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 365 AA; 41687 MW; 27E0B352983B595A CRC64;
MAVTQAVLPS FSNFCTNSEN MKFEKGFLDL DSKDLHQSPA SYKPSQFLTD EHQDDSEGCW
DMEFLLSDWA SMSPERSNSQ NYTPQRPTLQ DQLHSPDLYQ ELNAPKPNRH QVCVASSLAE
LLPPEATLSS SLPPDLQFNC GFLDQQAAKS YSQAENPQQF SAFPVTSNVN RDSSMGKMGK
SWDFGHYYQP APLISFPDSK FVQTQGITME TVVFPPHHHY NFIPSYSHPR LYQQQANYVH
RPPPQSHFPS TQSMVPDSTV PPAGLESKRI RRGLVKRKAT VHSCEYPGCQ KTYTKSSHLK
AHLRTHTGEK PYHCTWDGCG WKFARSDELT RHFRKHTGQK PYECLLCHRA FSRSDHLALH
MKRHV
