KLF7_MOUSE
ID KLF7_MOUSE Reviewed; 301 AA.
AC Q99JB0; Q99P62;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Krueppel-like factor 7;
GN Name=Klf7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=CD-1;
RX PubMed=11245580; DOI=10.1242/dev.128.7.1147;
RA Lei L., Ma L., Nef S., Thai T., Parada L.F.;
RT "mKlf7, a potential transcriptional regulator of TrkA nerve growth factor
RT receptor expression in sensory and sympathetic neurons.";
RL Development 128:1147-1158(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=Swiss Webster / NIH;
RX PubMed=11336497; DOI=10.1006/dbio.2001.0243;
RA Laub F.B., Aldabe R., Friedrich V. Jr., Ohnishi S., Yoshida T., Ramirez F.;
RT "Developmental expression of mouse Kruppel-like transcription factor KLF7
RT suggests a potential role in neurogenesis.";
RL Dev. Biol. 233:305-318(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP INTERACTION WITH FBXO38.
RX PubMed=14729953; DOI=10.1128/mcb.24.3.1058-1069.2004;
RA Smaldone S., Laub F., Else C., Dragomir C., Ramirez F.;
RT "Identification of MoKA, a novel F-box protein that modulates Krueppel-like
RT transcription factor 7 activity.";
RL Mol. Cell. Biol. 24:1058-1069(2004).
RN [5]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=15964824; DOI=10.1128/mcb.25.13.5699-5711.2005;
RA Laub F., Lei L., Sumiyoshi H., Kajimura D., Dragomir C., Smaldone S.,
RA Puche A.C., Petros T.J., Mason C., Parada L.F., Ramirez F.;
RT "Transcription factor KLF7 is important for neuronal morphogenesis in
RT selected regions of the nervous system.";
RL Mol. Cell. Biol. 25:5699-5711(2005).
RN [6]
RP DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX PubMed=28916725; DOI=10.1074/jbc.m117.793117;
RA Klein R.H., Hu W., Kashgari G., Lin Z., Nguyen T., Doan M., Andersen B.;
RT "Characterization of enhancers and the role of the transcription factor
RT KLF7 in regulating corneal epithelial differentiation.";
RL J. Biol. Chem. 292:18937-18950(2017).
CC -!- FUNCTION: Transcriptional factor (PubMed:11245580). Plays a critical
CC role in neuronal morphogenesis (PubMed:11336497, PubMed:15964824).
CC Represses the corneal epithelium differentiation (By similarity). Acts
CC also as a metabolic regulator, by modulating insulin sensitivity in
CC pancreatic beta cells and skeletal muscle cells. Inhibits
CC transcriptional inducers of adipogenesis and has a repressive role in
CC the expression of several adipokines, including leptin (By similarity).
CC {ECO:0000250|UniProtKB:O75840, ECO:0000269|PubMed:11245580,
CC ECO:0000269|PubMed:11336497, ECO:0000269|PubMed:15964824}.
CC -!- SUBUNIT: Interacts with FBXO38. {ECO:0000269|PubMed:14729953}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O75840}.
CC -!- TISSUE SPECIFICITY: Widely expressed (PubMed:11336497). Detected in the
CC cornea epithelium (at protein level) (PubMed:28916725).
CC {ECO:0000269|PubMed:11336497, ECO:0000269|PubMed:28916725}.
CC -!- DEVELOPMENTAL STAGE: Expression starts at 8.5 dpc in the trigeminal
CC ganglion, the VII-VIII neural crest complex, and the subventricular
CC neuroepithelium of both forebrain and hindbrain regions. At 11.5 dpc,
CC expression is detected in the neural crest-derived sensory nervous
CC system and, at 15.5 dpc, in the brain, spinal cord, retinal
CC neuroepithelium and the inferior XI/X complex (PubMed:11336497,
CC PubMed:11245580). In postnatal and adult animals, expression is
CC confined to the cerebellum and dorsal root ganglia (PubMed:11336497).
CC Expressed in the cornea epithelium at both 18.5 dpc and early postnatal
CC (P5) but not at P50, when the corneal epithelium is fully
CC differentiated (PubMed:28916725). {ECO:0000269|PubMed:11245580,
CC ECO:0000269|PubMed:11336497, ECO:0000269|PubMed:28916725}.
CC -!- DOMAIN: The acidic N-terminal part may favor interaction with the basic
CC domain of transcription factors. {ECO:0000250|UniProtKB:O75840}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors. In KLF7, the motif is
CC inactive. {ECO:0000250|UniProtKB:O75840}.
CC -!- DISRUPTION PHENOTYPE: Deficient mice die within the first 3 days of
CC life, showing little or no milk in the stomachs, hypopnea, cyanosis,
CC olfactory bulb hypoplasia, axon outgrowth defects in the olfactory and
CC optic nerves, defects of axon growth in the brain and reduced dendritic
CC branching in the hippocampus. {ECO:0000269|PubMed:28916725}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AF338369; AAK31139.1; -; mRNA.
DR EMBL; AF327568; AAK08964.1; -; mRNA.
DR EMBL; BC004700; AAH04700.1; -; mRNA.
DR EMBL; BC019604; AAH19604.1; -; mRNA.
DR EMBL; BC040794; AAH40794.1; -; mRNA.
DR CCDS; CCDS15003.1; -.
DR RefSeq; NP_291041.2; NM_033563.2.
DR RefSeq; XP_006496417.1; XM_006496354.3.
DR AlphaFoldDB; Q99JB0; -.
DR SMR; Q99JB0; -.
DR STRING; 10090.ENSMUSP00000109720; -.
DR iPTMnet; Q99JB0; -.
DR PhosphoSitePlus; Q99JB0; -.
DR MaxQB; Q99JB0; -.
DR PaxDb; Q99JB0; -.
DR PRIDE; Q99JB0; -.
DR ProteomicsDB; 263621; -.
DR Antibodypedia; 34186; 258 antibodies from 28 providers.
DR DNASU; 93691; -.
DR Ensembl; ENSMUST00000114086; ENSMUSP00000109720; ENSMUSG00000025959.
DR GeneID; 93691; -.
DR KEGG; mmu:93691; -.
DR UCSC; uc007bgo.2; mouse.
DR CTD; 8609; -.
DR MGI; MGI:1935151; Klf7.
DR VEuPathDB; HostDB:ENSMUSG00000025959; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000155235; -.
DR HOGENOM; CLU_002678_33_4_1; -.
DR InParanoid; Q99JB0; -.
DR OMA; FLHATPH; -.
DR OrthoDB; 982312at2759; -.
DR PhylomeDB; Q99JB0; -.
DR TreeFam; TF350556; -.
DR BioGRID-ORCS; 93691; 4 hits in 75 CRISPR screens.
DR ChiTaRS; Klf7; mouse.
DR PRO; PR:Q99JB0; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q99JB0; protein.
DR Bgee; ENSMUSG00000025959; Expressed in rostral migratory stream and 261 other tissues.
DR ExpressionAtlas; Q99JB0; baseline and differential.
DR Genevisible; Q99JB0; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IDA:MGI.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0007411; P:axon guidance; IMP:MGI.
DR GO; GO:0007409; P:axonogenesis; IMP:MGI.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:MGI.
DR GO; GO:0042593; P:glucose homeostasis; ISS:UniProtKB.
DR GO; GO:1904178; P:negative regulation of adipose tissue development; ISS:UniProtKB.
DR GO; GO:0061179; P:negative regulation of insulin secretion involved in cellular response to glucose stimulus; ISS:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0045604; P:regulation of epidermal cell differentiation; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..301
FT /note="Krueppel-like factor 7"
FT /id="PRO_0000047175"
FT ZN_FING 218..242
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 248..272
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 278..300
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT MOTIF 93..101
FT /note="9aaTAD; inactive"
FT /evidence="ECO:0000250|UniProtKB:O75840"
FT MOTIF 214..219
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT CONFLICT 175
FT /note="S -> P (in Ref. 2; AAK08964)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 301 AA; 33337 MW; 3AEB7135A9F6E6E7 CRC64;
MDVLASYSIF QELQLVHDTG YFSALPSLEE TWQQTCLELE RYLQTEPRRI SETFGEDLDC
FLHASPPPCI EESFRRLDPL LLPVEATICE KSSAVDILLS RDKLLSETCL SLQPTSSSLD
SYTAVNQAQL NAVTSLTPPS SPELSRHLVK TSQTLSAVDG TVTLKLVAKK ASLSSVKVGG
VAAAAAVTPA GAVKSGQSDS EQGGGGADTC PENKKRVHRC QFNGCRKVYT KSSHLKAHQR
THTGEKPYKC SWEGCEWRFA RSDELTRHYR KHTGAKPFKC NHCDRCFSRS DHLALHMKRH
I
