KLF8_MOUSE
ID KLF8_MOUSE Reviewed; 355 AA.
AC Q8BLM0;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Krueppel-like factor 8;
GN Name=Klf8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain cortex;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcriptional repressor and activator. Binds to CACCC-boxes
CC promoter elements. Also binds the GT-box of cyclin D1 promoter and
CC mediates cell cycle progression at G(1) phase as a downstream target of
CC focal adhesion kinase (FAK) (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with corepressor CtBP2. Interacts with PIAS1, PIAS2,
CC AND PIAS4; the interaction with each ligase sumoylates KLF8 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The 9aaTAD motif is a transactivation domain present in a large
CC number of yeast and animal transcription factors. In KLF8, the motif is
CC inactive. {ECO:0000250|UniProtKB:O95600}.
CC -!- PTM: Sumoylation at Lys-64 represses transcriptional activity and
CC reduces cell cycle progression into the G(1) phase. Has no effect on
CC subcellular location.
CC -!- SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; AK044185; BAC31807.1; -; mRNA.
DR EMBL; BC070442; AAH70442.1; -; mRNA.
DR CCDS; CCDS30481.1; -.
DR RefSeq; NP_776141.1; NM_173780.4.
DR RefSeq; XP_006528916.1; XM_006528853.3.
DR RefSeq; XP_006528921.1; XM_006528858.3.
DR RefSeq; XP_006528922.1; XM_006528859.3.
DR RefSeq; XP_006528923.1; XM_006528860.3.
DR RefSeq; XP_011246123.1; XM_011247821.2.
DR RefSeq; XP_017173992.1; XM_017318503.1.
DR RefSeq; XP_017173993.1; XM_017318504.1.
DR RefSeq; XP_017173994.1; XM_017318505.1.
DR RefSeq; XP_017173995.1; XM_017318506.1.
DR AlphaFoldDB; Q8BLM0; -.
DR SMR; Q8BLM0; -.
DR STRING; 10090.ENSMUSP00000108193; -.
DR iPTMnet; Q8BLM0; -.
DR PhosphoSitePlus; Q8BLM0; -.
DR PaxDb; Q8BLM0; -.
DR PeptideAtlas; Q8BLM0; -.
DR PRIDE; Q8BLM0; -.
DR ProteomicsDB; 264998; -.
DR Antibodypedia; 13002; 225 antibodies from 32 providers.
DR DNASU; 245671; -.
DR Ensembl; ENSMUST00000039545; ENSMUSP00000044317; ENSMUSG00000041649.
DR Ensembl; ENSMUST00000112574; ENSMUSP00000108193; ENSMUSG00000041649.
DR GeneID; 245671; -.
DR KEGG; mmu:245671; -.
DR UCSC; uc009uqu.1; mouse.
DR CTD; 11279; -.
DR MGI; MGI:2442430; Klf8.
DR VEuPathDB; HostDB:ENSMUSG00000041649; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000161062; -.
DR HOGENOM; CLU_002678_33_0_1; -.
DR InParanoid; Q8BLM0; -.
DR OMA; KMTSPPF; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q8BLM0; -.
DR TreeFam; TF350556; -.
DR BioGRID-ORCS; 245671; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Klf8; mouse.
DR PRO; PR:Q8BLM0; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q8BLM0; protein.
DR Bgee; ENSMUSG00000041649; Expressed in embryonic brain and 61 other tissues.
DR ExpressionAtlas; Q8BLM0; baseline and differential.
DR GO; GO:0016235; C:aggresome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0003677; F:DNA binding; ISS:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Reference proteome;
KW Repeat; Repressor; Transcription; Transcription regulation;
KW Ubl conjugation; Zinc; Zinc-finger.
FT CHAIN 1..355
FT /note="Krueppel-like factor 8"
FT /id="PRO_0000294168"
FT ZN_FING 270..294
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 300..324
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 330..352
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 19..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 82..90
FT /note="9aaTAD; inactive"
FT /evidence="ECO:0000250|UniProtKB:O95600"
FT CROSSLNK 64
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO)"
FT /evidence="ECO:0000250"
SQ SEQUENCE 355 AA; 38845 MW; 8DAA415948F6128F CRC64;
MDKFIDNMDV RIKSESGSMQ VFKQVTGPVP TRDPSARADR RNMTSPSFLA ASPMENPALF
NDIKIEPPEE LLESDFNMPQ VEPVDLSFHK PKAPLQPASM LQAPIRPPKP PTAPQAIMVP
TSADTVTSAA IPTVLTPGSI LASSQGTGGQ PILHVIHTIP SVSLPNKMSG LKTIPLVVQS
LPMVYTSLPT DGSPAAITVP LIGGDGKSAG SVKVDPASMC PLEFPSDSDE SAIESGSSAL
QSLQGFHHEP ATMVHMQGEE SLDLKRRRIH QCDFAGCSKV YTKSSHLKAH RRIHTGEKPY
KCTWDGCSWK FARSDELTRH FRKHTGIKPF RCTDCNRSFS RSDHLSLHRR RHDTM
