KLF9_HUMAN
ID KLF9_HUMAN Reviewed; 244 AA.
AC Q13886; B2R943; Q16196;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Krueppel-like factor 9;
DE AltName: Full=Basic transcription element-binding protein 1;
DE Short=BTE-binding protein 1;
DE AltName: Full=GC-box-binding protein 1;
DE AltName: Full=Transcription factor BTEB1;
GN Name=KLF9; Synonyms=BTEB, BTEB1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8291025; DOI=10.1007/bf01233255;
RA Ohe N., Yamasaki Y., Sogawa K., Inazawa J., Ariyama T., Oshimura M.,
RA Fujii-Kuriyama Y.;
RT "Chromosomal localization and cDNA sequence of human BTEB, a GC box binding
RT protein.";
RL Somat. Cell Mol. Genet. 19:499-503(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-31.
RX PubMed=8051167; DOI=10.1016/s0021-9258(17)32045-8;
RA Imataka H., Nakayama K., Yasumoto K., Mizuno A., Fujii-Kuriyama Y.,
RA Hayami M.;
RT "Cell-specific translational control of transcription factor BTEB
RT expression. The role of an upstream AUG in the 5'-untranslated region.";
RL J. Biol. Chem. 269:20668-20673(1994).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=22711835; DOI=10.1073/pnas.1118641109;
RA Sporl F., Korge S., Jurchott K., Wunderskirchner M., Schellenberg K.,
RA Heins S., Specht A., Stoll C., Klemz R., Maier B., Wenck H., Schrader A.,
RA Kunz D., Blatt T., Kramer A.;
RT "Kruppel-like factor 9 is a circadian transcription factor in human
RT epidermis that controls proliferation of keratinocytes.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:10903-10908(2012).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-122, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [10]
RP INTERACTION WITH ZZEF1.
RX PubMed=33227311; DOI=10.1016/j.jmb.2020.11.021;
RA Yu Y., Tencer A., Xuan H., Kutateladze T.G., Shi X.;
RT "ZZEF1 is a Histone Reader and Transcriptional Coregulator of Krueppel-Like
RT Factors.";
RL J. Mol. Biol. 433:166722-166722(2021).
CC -!- FUNCTION: Transcription factor that binds to GC box promoter elements.
CC Selectively activates mRNA synthesis from genes containing tandem
CC repeats of GC boxes but represses genes with a single GC box. Acts as
CC an epidermal circadian transcription factor regulating keratinocyte
CC proliferation (PubMed:22711835). {ECO:0000269|PubMed:22711835}.
CC -!- SUBUNIT: Interacts with ZZEF1. {ECO:0000269|PubMed:33227311}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:22711835}.
CC -!- TISSUE SPECIFICITY: Epidermis (at protein level).
CC {ECO:0000269|PubMed:22711835}.
CC -!- INDUCTION: Expression is highly sensitive to glucocorticoids and shows
CC diurnal expression patterns. A strong induction of expression seen
CC during keratinocyte differentiation in a cortisol dependent manner.
CC {ECO:0000269|PubMed:22711835}.
CC -!- SIMILARITY: Belongs to the Sp1 C2H2-type zinc-finger protein family.
CC {ECO:0000305}.
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DR EMBL; D31716; BAA06524.1; -; mRNA.
DR EMBL; AK313631; BAG36390.1; -; mRNA.
DR EMBL; AL162390; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471089; EAW62503.1; -; Genomic_DNA.
DR EMBL; BC069431; AAH69431.1; -; mRNA.
DR EMBL; BC074879; AAH74879.1; -; mRNA.
DR EMBL; BC074880; AAH74880.1; -; mRNA.
DR EMBL; S72504; AAD14110.1; -; mRNA.
DR CCDS; CCDS6633.1; -.
DR PIR; I59602; I59602.
DR RefSeq; NP_001197.1; NM_001206.2.
DR AlphaFoldDB; Q13886; -.
DR SMR; Q13886; -.
DR BioGRID; 107152; 89.
DR ELM; Q13886; -.
DR IntAct; Q13886; 82.
DR STRING; 9606.ENSP00000366330; -.
DR iPTMnet; Q13886; -.
DR PhosphoSitePlus; Q13886; -.
DR BioMuta; KLF9; -.
DR DMDM; 3913177; -.
DR EPD; Q13886; -.
DR jPOST; Q13886; -.
DR MassIVE; Q13886; -.
DR MaxQB; Q13886; -.
DR PaxDb; Q13886; -.
DR PeptideAtlas; Q13886; -.
DR PRIDE; Q13886; -.
DR ProteomicsDB; 59714; -.
DR Antibodypedia; 12411; 257 antibodies from 28 providers.
DR DNASU; 687; -.
DR Ensembl; ENST00000377126.4; ENSP00000366330.2; ENSG00000119138.5.
DR GeneID; 687; -.
DR KEGG; hsa:687; -.
DR MANE-Select; ENST00000377126.4; ENSP00000366330.2; NM_001206.4; NP_001197.1.
DR UCSC; uc004aht.4; human.
DR CTD; 687; -.
DR DisGeNET; 687; -.
DR GeneCards; KLF9; -.
DR HGNC; HGNC:1123; KLF9.
DR HPA; ENSG00000119138; Low tissue specificity.
DR MIM; 602902; gene.
DR neXtProt; NX_Q13886; -.
DR OpenTargets; ENSG00000119138; -.
DR PharmGKB; PA25444; -.
DR VEuPathDB; HostDB:ENSG00000119138; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000160905; -.
DR HOGENOM; CLU_002678_33_2_1; -.
DR InParanoid; Q13886; -.
DR OMA; DPRDAWK; -.
DR OrthoDB; 1308225at2759; -.
DR PhylomeDB; Q13886; -.
DR TreeFam; TF351003; -.
DR PathwayCommons; Q13886; -.
DR SignaLink; Q13886; -.
DR SIGNOR; Q13886; -.
DR BioGRID-ORCS; 687; 13 hits in 1099 CRISPR screens.
DR ChiTaRS; KLF9; human.
DR GeneWiki; KLF9; -.
DR GenomeRNAi; 687; -.
DR Pharos; Q13886; Tbio.
DR PRO; PR:Q13886; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q13886; protein.
DR Bgee; ENSG00000119138; Expressed in calcaneal tendon and 203 other tissues.
DR ExpressionAtlas; Q13886; baseline and differential.
DR Genevisible; Q13886; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:ProtInc.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0071387; P:cellular response to cortisol stimulus; IDA:UniProtKB.
DR GO; GO:0097067; P:cellular response to thyroid hormone stimulus; IDA:UniProtKB.
DR GO; GO:0007623; P:circadian rhythm; IEP:UniProtKB.
DR GO; GO:0010839; P:negative regulation of keratinocyte proliferation; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 1: Evidence at protein level;
KW Biological rhythms; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..244
FT /note="Krueppel-like factor 9"
FT /id="PRO_0000047154"
FT ZN_FING 143..167
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 173..197
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 203..225
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 80..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 98..115
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 122
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
SQ SEQUENCE 244 AA; 27235 MW; 2D1B5A5BB9D42221 CRC64;
MSAAAYMDFV AAQCLVSISN RAAVPEHGVA PDAERLRLPE REVTKEHGDP GDTWKDYCTL
VTIAKSLLDL NKYRPIQTPS VCSDSLESPD EDMGSDSDVT TESGSSPSHS PEERQDPGSA
PSPLSLLHPG VAAKGKHASE KRHKCPYSGC GKVYGKSSHL KAHYRVHTGE RPFPCTWPDC
LKKFSRSDEL TRHYRTHTGE KQFRCPLCEK RFMRSDHLTK HARRHTEFHP SMIKRSKKAL
ANAL
