KLFL_DROME
ID KLFL_DROME Reviewed; 570 AA.
AC Q8MR37;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Krueppel-like factor luna {ECO:0000303|PubMed:14527717, ECO:0000303|PubMed:24915236};
GN Name=luna {ECO:0000312|FlyBase:FBgn0040765};
GN ORFNames=CG33473 {ECO:0000312|FlyBase:FBgn0040765};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:AAO85420.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=14527717; DOI=10.1016/s0378-1119(03)00720-0;
RA De Graeve F., Smaldone S., Laub F., Mlodzik M., Bhat M., Ramirez F.;
RT "Identification of the Drosophila progenitor of mammalian Kruppel-like
RT factors 6 and 7 and a determinant of fly development.";
RL Gene 314:55-62(2003).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:AAM52655.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM52655.1};
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=24915236; DOI=10.1371/journal.pone.0096933;
RA Weber U., Rodriguez E., Martignetti J., Mlodzik M.;
RT "Luna, a Drosophila KLF6/KLF7, is maternally required for synchronized
RT nuclear and centrosome cycles in the preblastoderm embryo.";
RL PLoS ONE 9:E96933-E96933(2014).
CC -!- FUNCTION: Probable transcription factor that is required for cell
CC differentiation (PubMed:14527717, PubMed:24915236). Essential for
CC proper separation of the sister chromatids during early nuclear
CC division cycles in the syncytial pre-blastoderm embryo
CC (PubMed:24915236). {ECO:0000269|PubMed:14527717,
CC ECO:0000269|PubMed:24915236}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305|PubMed:14527717}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos.
CC {ECO:0000269|PubMed:14527717}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in embryos is semi-
CC lethal, with half of the mutants dying prior to gastrulation and
CC display numerous vacuoles. Escaper embryos die at the third instar
CC larval or early pupal stages, displaying black necrotic plaques.
CC {ECO:0000269|PubMed:14527717}.
CC -!- MISCELLANEOUS: Named 'luna' based upon mutant embryo phenotype in which
CC large vacuoles formed in the egg yolk resemble the lunar surface.
CC {ECO:0000303|PubMed:14527717}.
CC -!- SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein
CC family. {ECO:0000305}.
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DR EMBL; AF461497; AAO85420.1; -; mRNA.
DR EMBL; AE013599; AAF58723.3; -; Genomic_DNA.
DR EMBL; AE013599; AGB93412.1; -; Genomic_DNA.
DR EMBL; AE013599; AHN56101.1; -; Genomic_DNA.
DR EMBL; AY122143; AAM52655.1; -; mRNA.
DR RefSeq; NP_001260879.1; NM_001273950.1.
DR RefSeq; NP_001286303.1; NM_001299374.1.
DR RefSeq; NP_995811.1; NM_206089.2.
DR AlphaFoldDB; Q8MR37; -.
DR SMR; Q8MR37; -.
DR IntAct; Q8MR37; 1.
DR STRING; 7227.FBpp0305280; -.
DR PaxDb; Q8MR37; -.
DR DNASU; 2768719; -.
DR EnsemblMetazoa; FBtr0088210; FBpp0087305; FBgn0040765.
DR EnsemblMetazoa; FBtr0333066; FBpp0305280; FBgn0040765.
DR EnsemblMetazoa; FBtr0345675; FBpp0311726; FBgn0040765.
DR GeneID; 2768719; -.
DR KEGG; dme:Dmel_CG33473; -.
DR UCSC; CG33473-RB; d. melanogaster.
DR CTD; 2768719; -.
DR FlyBase; FBgn0040765; luna.
DR VEuPathDB; VectorBase:FBgn0040765; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000170669; -.
DR HOGENOM; CLU_487692_0_0_1; -.
DR InParanoid; Q8MR37; -.
DR OMA; KSHHPRQ; -.
DR OrthoDB; 982312at2759; -.
DR PhylomeDB; Q8MR37; -.
DR SignaLink; Q8MR37; -.
DR BioGRID-ORCS; 2768719; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 2768719; -.
DR PRO; PR:Q8MR37; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0040765; Expressed in brain and 18 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:FlyBase.
DR GO; GO:0035185; P:preblastoderm mitotic cell cycle; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 3.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 3.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 3.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Developmental protein; Metal-binding; Mitosis;
KW Nucleus; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..570
FT /note="Krueppel-like factor luna"
FT /evidence="ECO:0000305"
FT /id="PRO_0000437247"
FT ZN_FING 487..511
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 517..541
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 547..569
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 138..182
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 268..297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 332..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 424..483
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..182
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 424..462
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 570 AA; 61770 MW; F889881AFBC85F3D CRC64;
MDILPSGNIF SELERICTTG YYSSQPSIED QWQQTCYELE RYLRDEPKLQ KKMHSNLDNP
WDIFSTPARL LEELKIKEQH LDTISTTSSV SSNCSGTSWD SNGSQALSCS VLVKQERIDE
EDDDVGCDDK LSVLLAAPPS AISPNPSIGS AGNMTPTSIS NSSSISSSNS HSNSNGNTTG
SSNIIASPLS SLGSGSTITV SSRNSSAPGI KVRVVQAKNH PGSNARMHLG HVQDFVLPTL
TPPSSPESNL RSHNYAQQLE ANDLAALIQQ QQQQQQQQQQ QQQQQQLNPQ PGNSTPATAI
LRFTSQSNPT MTQLQQQQQQ LAVQHLSISP QALGHSNIGS NSPKSSSSSS SNSSNSSSSS
HSSSSSISGS SDQPTHSNIP RSTIVRLTTA NGKPGAAGIS LARVIQMQNN GNANVAAVLS
AAANSGSSNS NSNSSSSNTS SNSINTATPQ QQVLSQRSEK SANGSSKPHH PRQHHSDHSP
DAKRRIHKCQ FLGCKKVYTK SSHLKAHQRT HTGEKPYKCS WEGCEWRFAR SDELTRHYRK
HTGAKPFKCR NCDRCFSRSD HLALHMKRHM
