KLH10_MOUSE
ID KLH10_MOUSE Reviewed; 608 AA.
AC Q9D5V2; Q8BVM3; Q9DA07;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Kelch-like protein 10;
GN Name=Klhl10;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=15136734; DOI=10.1073/pnas.0308025101;
RA Yan W., Ma L., Burns K.H., Matzuk M.M.;
RT "Haploinsufficiency of kelch-like protein homolog 10 causes infertility in
RT male mice.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7793-7798(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP FUNCTION, AND INTERACTION WITH CUL3.
RX PubMed=16162871; DOI=10.1095/biolreprod.105.045484;
RA Wang S., Zheng H., Esaki Y., Kelly F., Yan W.;
RT "Cullin3 is a KLHL10-interacting protein preferentially expressed during
RT late spermiogenesis.";
RL Biol. Reprod. 74:102-108(2006).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be a substrate-specific adapter of a CUL3-based E3
CC ubiquitin-protein ligase complex which mediates the ubiquitination and
CC subsequent proteasomal degradation of target proteins during
CC spermatogenesis. Required for male fertility.
CC {ECO:0000269|PubMed:15136734, ECO:0000269|PubMed:16162871}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Self-associates (By similarity). Interacts with CUL3;
CC indicative for the participation in an E3 ubiquitin ligase complex.
CC {ECO:0000250, ECO:0000269|PubMed:16162871}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15136734}.
CC -!- TISSUE SPECIFICITY: Testis specific. {ECO:0000269|PubMed:15136734}.
CC -!- DEVELOPMENTAL STAGE: Expressed in elongating and elongated spermatids
CC (steps 9-16). {ECO:0000269|PubMed:15136734}.
CC -!- DISRUPTION PHENOTYPE: Heterozygous males are completely infertile
CC because of disrupted spermiogenesis characterized by asynchronous
CC spermatid maturation, degeneration of late spermatids, sloughing of
CC postmeiotic germ cells from the seminiferous epithelium, and marked
CC reduction in the numbers of late spermatids.
CC {ECO:0000269|PubMed:15136734}.
CC -!- MISCELLANEOUS: Does not seem to associate with actin.
CC {ECO:0000305|PubMed:15136734}.
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DR EMBL; AY495337; AAS91790.1; -; mRNA.
DR EMBL; AK006288; BAB24507.1; -; mRNA.
DR EMBL; AK014906; BAB29614.1; -; mRNA.
DR EMBL; AK077202; BAC36680.1; -; mRNA.
DR CCDS; CCDS25423.1; -.
DR RefSeq; NP_080003.1; NM_025727.3.
DR AlphaFoldDB; Q9D5V2; -.
DR SMR; Q9D5V2; -.
DR BioGRID; 211671; 1.
DR STRING; 10090.ENSMUSP00000001599; -.
DR PhosphoSitePlus; Q9D5V2; -.
DR PaxDb; Q9D5V2; -.
DR PRIDE; Q9D5V2; -.
DR ProteomicsDB; 263650; -.
DR Antibodypedia; 29004; 49 antibodies from 12 providers.
DR DNASU; 66720; -.
DR Ensembl; ENSMUST00000001599; ENSMUSP00000001599; ENSMUSG00000001558.
DR GeneID; 66720; -.
DR KEGG; mmu:66720; -.
DR UCSC; uc007lli.1; mouse.
DR CTD; 317719; -.
DR MGI; MGI:2181067; Klhl10.
DR VEuPathDB; HostDB:ENSMUSG00000001558; -.
DR eggNOG; KOG4441; Eukaryota.
DR GeneTree; ENSGT00940000154664; -.
DR HOGENOM; CLU_004253_14_1_1; -.
DR InParanoid; Q9D5V2; -.
DR OMA; SRMCSGE; -.
DR OrthoDB; 277265at2759; -.
DR PhylomeDB; Q9D5V2; -.
DR TreeFam; TF329218; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 66720; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Klhl10; mouse.
DR PRO; PR:Q9D5V2; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9D5V2; protein.
DR Bgee; ENSMUSG00000001558; Expressed in seminiferous tubule of testis and 41 other tissues.
DR ExpressionAtlas; Q9D5V2; baseline and differential.
DR Genevisible; Q9D5V2; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0000902; P:cell morphogenesis; IMP:MGI.
DR GO; GO:0009566; P:fertilization; IMP:MGI.
DR GO; GO:0048873; P:homeostasis of number of cells within a tissue; IMP:MGI.
DR GO; GO:0048808; P:male genitalia morphogenesis; IMP:MGI.
DR GO; GO:0008584; P:male gonad development; IMP:MGI.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0007286; P:spermatid development; IMP:MGI.
DR CDD; cd18240; BTB_POZ_KLHL10; 1.
DR Gene3D; 2.120.10.80; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR017096; BTB-kelch_protein.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR030608; KLHL10_BTB/POZ.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF01344; Kelch_1; 6.
DR PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00612; Kelch; 6.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Differentiation; Kelch repeat; Phosphoprotein;
KW Reference proteome; Repeat; Spermatogenesis; Ubl conjugation pathway.
FT CHAIN 1..608
FT /note="Kelch-like protein 10"
FT /id="PRO_0000119113"
FT DOMAIN 39..106
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT REPEAT 292..339
FT /note="Kelch 1"
FT REPEAT 340..386
FT /note="Kelch 2"
FT REPEAT 388..433
FT /note="Kelch 3"
FT REPEAT 434..480
FT /note="Kelch 4"
FT REPEAT 481..527
FT /note="Kelch 5"
FT REPEAT 529..574
FT /note="Kelch 6"
FT MOD_RES 501
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6JEL3"
FT CONFLICT 159
FT /note="K -> T (in Ref. 2; BAC36680)"
FT /evidence="ECO:0000305"
FT CONFLICT 251
FT /note="D -> N (in Ref. 2; BAB24507)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 608 AA; 68977 MW; E7B8F1C4FF0DEF7A CRC64;
MEMESTAAST RFHQPHMERK MSAMTCEIFN ELRLEGKLCD VVIKVNGFEF NAHKNILCSC
SSYFRALFTS GWNNTEKKVY NIPGISPDMM KLIIEYAYTR TVPITPDNVE KLLAAADQFN
IMGIVRGCCE FLKSELCLDN CIGICKFTDY YYCPELRQKA YMFILHNFEE MVKVSAEFLE
LSVTELKDII EKDELNVKQE DAVFEAILKW ISHDPQNRKQ HISVLLPKVR LALMHAEYFM
NNVKMNDYVK DSEECKPVII NALKAMYDLN MNGPSNSDFT NPLTRPRLPY AILFAIGGWS
GGSPTNAIEA YDARADRWVN VTCEEESPRA YHGAAYLKGY VYIIGGFDSV DYFNSVKRFD
PVKKTWHQVA PMHSRRCYVS VTVLSNFIYA MGGFDGYVRL NTAERYEPET NQWTLIAPMH
EQRSDASATT LYGKVYICGG FNGNECLFTA EVYNTESNQW TVIAPMRSRR SGIGVIAYGE
HVYAVGGFDG ANRLRSAEAY SPVANTWRTI PTMFNPRSNF GIEVVDDLLF VVGGFNGFTT
TFNVECYDEK TDEWYDAHDM SIYRSALSCC VVPGLANVGE YAARRDNFTG LALRDEVKYS
ASTSTLPV
