ID   KLH12_XENLA             Reviewed;         564 AA.
AC   Q6NRH0;
DT   16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 2.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Kelch-like protein 12;
GN   Name=klhl12;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Oocyte;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC       ubiquitin ligase complex that acts as a negative regulator of Wnt
CC       signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is
CC       involved in ER-Golgi transport by regulating the size of COPII coats,
CC       thereby playing a key role in collagen export, which is required for
CC       embryonic stem (ES) cells division (By similarity).
CC       {ECO:0000250|UniProtKB:Q53G59}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Component of the BCR(KLHL12) E3 ubiquitin ligase complex.
CC       {ECO:0000250|UniProtKB:Q53G59}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, COPII-coated vesicle
CC       {ECO:0000250|UniProtKB:Q53G59}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAH70780.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC070780; AAH70780.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001084819.2; NM_001091350.1.
DR   RefSeq; XP_018100468.1; XM_018244979.1.
DR   RefSeq; XP_018100469.1; XM_018244980.1.
DR   AlphaFoldDB; Q6NRH0; -.
DR   SMR; Q6NRH0; -.
DR   PRIDE; Q6NRH0; -.
DR   DNASU; 431860; -.
DR   GeneID; 431860; -.
DR   CTD; 431860; -.
DR   Xenbase; XB-GENE-961998; klhl12.L.
DR   OMA; SLGTQRC; -.
DR   OrthoDB; 709680at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   Bgee; 431860; Expressed in muscle tissue and 19 other tissues.
DR   GO; GO:0030127; C:COPII vesicle coat; ISS:UniProtKB.
DR   GO; GO:0030134; C:COPII-coated ER to Golgi transport vesicle; ISS:UniProtKB.
DR   GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0048208; P:COPII vesicle coating; ISS:UniProtKB.
DR   GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; ISS:UniProtKB.
DR   GO; GO:0014032; P:neural crest cell development; ISS:UniProtKB.
DR   GO; GO:0014029; P:neural crest formation; ISS:UniProtKB.
DR   GO; GO:0006513; P:protein monoubiquitination; ISS:UniProtKB.
DR   GO; GO:0016055; P:Wnt signaling pathway; ISS:UniProtKB.
DR   Gene3D; 2.120.10.80; -; 1.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR011705; BACK.
DR   InterPro; IPR017096; BTB-kelch_protein.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   Pfam; PF07707; BACK; 1.
DR   Pfam; PF00651; BTB; 1.
DR   Pfam; PF01344; Kelch_1; 6.
DR   PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR   SMART; SM00875; BACK; 1.
DR   SMART; SM00225; BTB; 1.
DR   SMART; SM00612; Kelch; 6.
DR   SUPFAM; SSF117281; SSF117281; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Developmental protein; ER-Golgi transport;
KW   Kelch repeat; Reference proteome; Repeat; Transport;
KW   Ubl conjugation pathway; Wnt signaling pathway.
FT   CHAIN           1..564
FT                   /note="Kelch-like protein 12"
FT                   /id="PRO_0000234353"
FT   DOMAIN          29..96
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT   DOMAIN          131..232
FT                   /note="BACK"
FT   REPEAT          278..325
FT                   /note="Kelch 1"
FT   REPEAT          327..375
FT                   /note="Kelch 2"
FT   REPEAT          376..422
FT                   /note="Kelch 3"
FT   REPEAT          423..469
FT                   /note="Kelch 4"
FT   REPEAT          471..516
FT                   /note="Kelch 5"
FT   REPEAT          518..563
FT                   /note="Kelch 6"
SQ   SEQUENCE   564 AA;  63209 MW;  10D2F819136CC1ED CRC64;
     MAPKDIMTNS HAKSILNTMN SLRKSQTLCD VTLRVNLKDF PAHRIVLAAC SDYFCAMFTN
     ELSEKGKPYV DIQGLTSSTM EILLDFVYTE TVHVTVENVQ ELLPAACLLQ LKGVKQACCD
     FLESQLDPSN CLGIRDFAET HNCLELMQAA EVYSQKHFPE VVQHEEFMLL HQEEVEKLIH
     CDEIQINSEE PVFEAVINWV KHNRHEREKS LPQLLQYVRM PLLTPRYITD VIDAEPLIRC
     SLQCRDLVDE AKKFHLRPEL RSQMQGPRTR VRLGANEVLL VIGGFGSQQS PIDIVEKYDP
     KTQEWSVLPS ITRKRRYVAT VSLGDRVYVI GGYDGRSRLS SVECLDYTSE EDGVWYSVAP
     MNVRRGLAGA TTLGDMIYVS GGFDGSRRHT SMERYDPNID QWSMLGDMQT AREGAGLVVA
     NGVIYCLGGY DGLNILSSVE RYDPHTGHWS HVTPMATKRS GAGVSLLNDH IYVVGGFDGT
     AHLSSVEAYN IRTDSWTTMT SMTTPRCYVG ATVLRGRLYA IAGYDGNSLL NSVECYDPLI
     DSWAVVTSMA TQRCDAGVCV LREK