KLH13_MOUSE
ID KLH13_MOUSE Reviewed; 654 AA.
AC Q80TF4; A2AL86; A2AL87; A2AL88; A2AL89; Q9DBY7;
DT 04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Kelch-like protein 13;
DE AltName: Full=BTB and kelch domain-containing protein 2;
GN Name=Klhl13; Synonyms=Bklhd2, Kiaa1309;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Lung;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC ubiquitin-protein ligase complex required for mitotic progression and
CC cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates
CC the ubiquitination of AURKB and controls the dynamic behavior of AURKB
CC on mitotic chromosomes and thereby coordinates faithful mitotic
CC progression and completion of cytokinesis (By similarity).
CC {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Component of the BCR(KLHL9-KLHL13) E3 ubiquitin ligase
CC complex, at least composed of CUL3, KLHL9, KLHL13 and RBX1. Interacts
CC with AURKB (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q80TF4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q80TF4-2; Sequence=VSP_037535;
CC Name=3;
CC IsoId=Q80TF4-3; Sequence=VSP_037536;
CC Name=4;
CC IsoId=Q80TF4-4; Sequence=VSP_037537;
CC Name=5;
CC IsoId=Q80TF4-5; Sequence=VSP_037534;
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC65773.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK122491; BAC65773.1; ALT_INIT; mRNA.
DR EMBL; AK004677; BAB23465.1; -; mRNA.
DR EMBL; AL773552; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS40891.1; -. [Q80TF4-3]
DR CCDS; CCDS72348.1; -. [Q80TF4-2]
DR CCDS; CCDS81105.1; -. [Q80TF4-4]
DR RefSeq; NP_001277405.1; NM_001290476.2. [Q80TF4-2]
DR RefSeq; NP_001297395.1; NM_001310466.1. [Q80TF4-4]
DR RefSeq; NP_080443.1; NM_026167.4. [Q80TF4-3]
DR RefSeq; XP_011245791.1; XM_011247489.2.
DR RefSeq; XP_011245794.1; XM_011247492.2. [Q80TF4-3]
DR RefSeq; XP_011245795.1; XM_011247493.2.
DR RefSeq; XP_011245796.1; XM_011247494.2.
DR RefSeq; XP_017174099.1; XM_017318610.1. [Q80TF4-3]
DR AlphaFoldDB; Q80TF4; -.
DR SMR; Q80TF4; -.
DR IntAct; Q80TF4; 2.
DR MINT; Q80TF4; -.
DR STRING; 10090.ENSMUSP00000110972; -.
DR iPTMnet; Q80TF4; -.
DR PhosphoSitePlus; Q80TF4; -.
DR MaxQB; Q80TF4; -.
DR PaxDb; Q80TF4; -.
DR PRIDE; Q80TF4; -.
DR ProteomicsDB; 264770; -. [Q80TF4-1]
DR ProteomicsDB; 264771; -. [Q80TF4-2]
DR ProteomicsDB; 264772; -. [Q80TF4-3]
DR ProteomicsDB; 264773; -. [Q80TF4-4]
DR ProteomicsDB; 264774; -. [Q80TF4-5]
DR Antibodypedia; 29658; 141 antibodies from 25 providers.
DR DNASU; 67455; -.
DR Ensembl; ENSMUST00000035973; ENSMUSP00000041190; ENSMUSG00000036782. [Q80TF4-3]
DR Ensembl; ENSMUST00000115313; ENSMUSP00000110968; ENSMUSG00000036782. [Q80TF4-5]
DR Ensembl; ENSMUST00000115316; ENSMUSP00000110971; ENSMUSG00000036782. [Q80TF4-4]
DR Ensembl; ENSMUST00000115317; ENSMUSP00000110972; ENSMUSG00000036782. [Q80TF4-1]
DR Ensembl; ENSMUST00000115319; ENSMUSP00000110974; ENSMUSG00000036782. [Q80TF4-2]
DR GeneID; 67455; -.
DR KEGG; mmu:67455; -.
DR UCSC; uc009sut.1; mouse. [Q80TF4-2]
DR UCSC; uc009suu.1; mouse. [Q80TF4-3]
DR CTD; 90293; -.
DR MGI; MGI:1914705; Klhl13.
DR VEuPathDB; HostDB:ENSMUSG00000036782; -.
DR eggNOG; KOG4441; Eukaryota.
DR GeneTree; ENSGT00940000154359; -.
DR HOGENOM; CLU_004253_14_3_1; -.
DR InParanoid; Q80TF4; -.
DR OMA; XGITHDT; -.
DR OrthoDB; 250404at2759; -.
DR PhylomeDB; Q80TF4; -.
DR TreeFam; TF328485; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 67455; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Klhl13; mouse.
DR PRO; PR:Q80TF4; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q80TF4; protein.
DR Bgee; ENSMUSG00000036782; Expressed in caudate-putamen and 244 other tissues.
DR Genevisible; Q80TF4; MM.
DR GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0097602; F:cullin family protein binding; ISO:MGI.
DR GO; GO:0047485; F:protein N-terminus binding; ISO:MGI.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISO:MGI.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0032465; P:regulation of cytokinesis; ISS:UniProtKB.
DR Gene3D; 2.120.10.80; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR017096; BTB-kelch_protein.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF01344; Kelch_1; 5.
DR PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00612; Kelch; 6.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell cycle; Cell division; Kelch repeat; Mitosis;
KW Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..654
FT /note="Kelch-like protein 13"
FT /id="PRO_0000119116"
FT DOMAIN 91..160
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 195..296
FT /note="BACK"
FT REPEAT 340..388
FT /note="Kelch 1"
FT REPEAT 389..440
FT /note="Kelch 2"
FT REPEAT 441..487
FT /note="Kelch 3"
FT REPEAT 489..534
FT /note="Kelch 4"
FT REPEAT 536..586
FT /note="Kelch 5"
FT REPEAT 587..635
FT /note="Kelch 6"
FT VAR_SEQ 1..50
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000305"
FT /id="VSP_037534"
FT VAR_SEQ 1..33
FT /note="MPLKWKTSSPAIWKFPVPVLKTSRSTPLSPAYI -> MLRFISHLYCCSSKE
FT ECSEDDKCILSR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12693553"
FT /id="VSP_037535"
FT VAR_SEQ 1..33
FT /note="MPLKWKTSSPAIWKFPVPVLKTSRSTPLSPAYI -> MDILHRGELVAAILR
FT NR (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_037536"
FT VAR_SEQ 1..33
FT /note="MPLKWKTSSPAIWKFPVPVLKTSRSTPLSPAYI -> MEGEYFFEENPFLEF
FT LLKL (in isoform 4)"
FT /evidence="ECO:0000305"
FT /id="VSP_037537"
SQ SEQUENCE 654 AA; 73787 MW; 33E8497FEF1571D2 CRC64;
MPLKWKTSSP AIWKFPVPVL KTSRSTPLSP AYISLVEEED QHLKLCLGSE MGLSSHLQSC
KAGSTRIFTS NSHSSVVLQG FDQLRLDGLL CDVTLMPGDT DDAYPVHRVM MASASDYFKA
MFTGGMKEQE LMCIKLHGVS RVGLRKIIDF IYTAKLSLNM DTLQDTLEAA SFLQILPVLD
FCKVFLISGV TLDNCVEVGR IANTYHLTEV DKYVNSFVLK NFAALLSTGE FLKLPFERLA
FVLSSNSLKR CTELDLFKAT CRWLRLEEPR MDVAAKLMKN IRFPLMTPQE LINYVQTVDF
MRTDNTCVNL LLEASNYQMM PFMQPVMQSD RTAIRSDTTR LVTLGGVLRQ QLVVSKELRM
YDEKTHEWKS LAPMDAPRYQ HGIAVIGNFL YVVGGQSNYD TKGKTAVDTV FRFDPRYNKW
IQVASLNEKR TFFHLSALKG FLYAVGGRNA AGELPTVECY NPRTNEWTYV AKMNEPHYGH
AGTVYGGVMY ISGGITHDTF QKELMCFDPD TDKWTQKAPM TTVRGLHCMC TVGDRLYVIG
GNHFRGTSDY DDVLSCEYYS PILDQWTPIA SMLRGQSDVG VAVFENKIYV VGGYSWNNRC
MVEIVQKYDP EKNEWHKVFD LPESLGGIRA CTLTVYPPEE ATPSPSRESP LSGP
