ID   KLH20_XENLA             Reviewed;         604 AA.
AC   Q6DFF6;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Kelch-like protein 20;
GN   Name=klhl20;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Oocyte;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC       ubiquitin-protein ligase complex involved in interferon response and
CC       anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin
CC       ligase complex mediates the ubiquitination of target proteins, leading
CC       to their degradation by the proteasome. It also specifically mediates
CC       'Lys-33'-linked ubiquitination (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Component of the BCR(KLHL20) E3 ubiquitin ligase complex, at
CC       least composed of cul3, klhl20 and rbx1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region {ECO:0000250}.
CC       Nucleus {ECO:0000250}.
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DR   EMBL; BC076782; AAH76782.1; -; mRNA.
DR   RefSeq; NP_001086544.1; NM_001093075.1.
DR   RefSeq; XP_018112263.1; XM_018256774.1.
DR   AlphaFoldDB; Q6DFF6; -.
DR   SMR; Q6DFF6; -.
DR   DNASU; 446379; -.
DR   GeneID; 446379; -.
DR   KEGG; xla:446379; -.
DR   CTD; 446379; -.
DR   Xenbase; XB-GENE-960639; klhl20.L.
DR   OMA; NSWSPIV; -.
DR   OrthoDB; 731760at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000186698; Chromosome 4L.
DR   Bgee; 446379; Expressed in blastula and 19 other tissues.
DR   GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016605; C:PML body; ISS:UniProtKB.
DR   GO; GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
DR   GO; GO:0019964; F:interferon-gamma binding; ISS:UniProtKB.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB.
DR   GO; GO:0006895; P:Golgi to endosome transport; ISS:UniProtKB.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR   GO; GO:1990390; P:protein K33-linked ubiquitination; ISS:UniProtKB.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR   Gene3D; 2.120.10.80; -; 1.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR011705; BACK.
DR   InterPro; IPR017096; BTB-kelch_protein.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR011043; Gal_Oxase/kelch_b-propeller.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR030594; KLHL17/KLHL20.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   PANTHER; PTHR24412:SF454; PTHR24412:SF454; 2.
DR   Pfam; PF07707; BACK; 1.
DR   Pfam; PF00651; BTB; 1.
DR   Pfam; PF01344; Kelch_1; 6.
DR   PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR   SMART; SM00875; BACK; 1.
DR   SMART; SM00225; BTB; 1.
DR   SMART; SM00612; Kelch; 6.
DR   SUPFAM; SSF50965; SSF50965; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Kelch repeat; Nucleus; Protein transport; Reference proteome;
KW   Repeat; Transport; Ubl conjugation pathway.
FT   CHAIN           1..604
FT                   /note="Kelch-like protein 20"
FT                   /id="PRO_0000396630"
FT   DOMAIN          63..130
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT   DOMAIN          165..267
FT                   /note="BACK"
FT   REPEAT          314..360
FT                   /note="Kelch 1"
FT   REPEAT          362..408
FT                   /note="Kelch 2"
FT   REPEAT          409..455
FT                   /note="Kelch 3"
FT   REPEAT          457..502
FT                   /note="Kelch 4"
FT   REPEAT          504..549
FT                   /note="Kelch 5"
FT   REPEAT          551..596
FT                   /note="Kelch 6"
SQ   SEQUENCE   604 AA;  67430 MW;  6364280A2C8481B0 CRC64;
     MRRCLNTRPG ETGMDVTSRC TLGDPNKLPE GVPQPARMPY VSDKHPRQTL EVINLLRKHR
     ELCDVVLVVG AKKIYAHRVI LSACSPYFRA MFTGELAESR QTEVVIRDID ERAMELLIDF
     SYTSQITVEE GNVQTLLPAA CLLQLAEIQE ACCEFLKRQL DPSNCLGIRA FADTHSCREL
     LRIADKFTQH NFQEVMESEE FMLLPANQLI DIISSDELNV RSEEQVFNAV MAWVKYSIQE
     RRPQLPQVLQ HVRLPLLSPK FLVGTVGSDP LIKSDEECRD LVDEAKNYLL LPQERPLMQG
     PRTRPRKPIR CGEVLFAVGG WCSGDAISSV ERYDPQTNEW RMVASMSKRR CGVGVSVLDD
     LLYAVGGHDG SSYLNSVERY DPKTNQWSSD VAPTSTCRTS VGVAVLGGYL YAVGGQDGVS
     CLNIVERYDP KENKWTRVAS MSTRRLGVAV AVLGGFLYAV GGSDGTSPLN TVERYNPQEN
     RWHTIAPMGT RRKHLGCAVY QDMIYAVGGR DDTTELSSAE RYNPRTNQWS PVVAMTSRRS
     GVGLAVVNGQ LMAVGGFDGT TYLKTIEVFD PDANTWRLYG GMNYRRLGGG VGVIKMTHCE
     SHIW