ID   KLH21_DANRE             Reviewed;         613 AA.
AC   Q6NYM1;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Kelch-like protein 21;
GN   Name=klhl21; ORFNames=zgc:76933;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (DEC-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Substrate-specific adapter of BCR (BTB-CUL3-RBX1) E3
CC       ubiquitin-protein ligase complex required for efficient chromosome
CC       alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex
CC       regulates localization of the chromosomal passenger complex (CPC) from
CC       chromosomes to the spindle midzone in anaphase and mediates the
CC       ubiquitination of AURKB (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at
CC       least composed of cul3, klhl21 and rbx1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle {ECO:0000250}.
CC       Note=Localizes to the spindle midzone and targets CUL3 to this region.
CC       {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC066537; AAH66537.1; -; mRNA.
DR   EMBL; BC155818; AAI55819.1; -; mRNA.
DR   RefSeq; NP_001307336.1; NM_001320407.1.
DR   RefSeq; XP_005162217.1; XM_005162160.3.
DR   AlphaFoldDB; Q6NYM1; -.
DR   SMR; Q6NYM1; -.
DR   STRING; 7955.ENSDARP00000115156; -.
DR   PaxDb; Q6NYM1; -.
DR   Ensembl; ENSDART00000137486; ENSDARP00000121765; ENSDARG00000039255.
DR   Ensembl; ENSDART00000140335; ENSDARP00000115156; ENSDARG00000039255.
DR   GeneID; 404613; -.
DR   KEGG; dre:404613; -.
DR   CTD; 9903; -.
DR   ZFIN; ZDB-GENE-040426-2627; klhl21.
DR   eggNOG; KOG4441; Eukaryota.
DR   GeneTree; ENSGT00940000158631; -.
DR   HOGENOM; CLU_004253_14_2_1; -.
DR   InParanoid; Q6NYM1; -.
DR   OMA; TWSVVGQ; -.
DR   OrthoDB; 743142at2759; -.
DR   PhylomeDB; Q6NYM1; -.
DR   TreeFam; TF329218; -.
DR   Reactome; R-DRE-8951664; Neddylation.
DR   Reactome; R-DRE-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:Q6NYM1; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 23.
DR   Bgee; ENSDARG00000039255; Expressed in muscle tissue and 22 other tissues.
DR   ExpressionAtlas; Q6NYM1; baseline.
DR   GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005827; C:polar microtubule; ISS:UniProtKB.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0035853; P:chromosome passenger complex localization to spindle midzone; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0032465; P:regulation of cytokinesis; ISS:UniProtKB.
DR   Gene3D; 2.120.10.80; -; 1.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR011705; BACK.
DR   InterPro; IPR017096; BTB-kelch_protein.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR030577; KLHL21.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   PANTHER; PTHR24412:SF255; PTHR24412:SF255; 1.
DR   Pfam; PF07707; BACK; 1.
DR   Pfam; PF00651; BTB; 1.
DR   Pfam; PF01344; Kelch_1; 2.
DR   PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR   SMART; SM00875; BACK; 1.
DR   SMART; SM00225; BTB; 1.
DR   SMART; SM00612; Kelch; 5.
DR   SUPFAM; SSF117281; SSF117281; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Kelch repeat; Mitosis;
KW   Reference proteome; Repeat; Ubl conjugation pathway.
FT   CHAIN           1..613
FT                   /note="Kelch-like protein 21"
FT                   /id="PRO_0000396632"
FT   DOMAIN          38..105
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT   DOMAIN          140..242
FT                   /note="BACK"
FT   REPEAT          289..337
FT                   /note="Kelch 1"
FT   REPEAT          338..384
FT                   /note="Kelch 2"
FT   REPEAT          386..424
FT                   /note="Kelch 3"
FT   REPEAT          426..472
FT                   /note="Kelch 4"
FT   REPEAT          474..514
FT                   /note="Kelch 5"
FT   REPEAT          515..562
FT                   /note="Kelch 6"
SQ   SEQUENCE   613 AA;  69737 MW;  D83AFF765D17BC0D CRC64;
     MEKPVLQTQP SMLPFFDTAH AVNLLRGIHE LRAERKFFDV TLCAEGKEFH CHRTVLAAAS
     MYFRAMFAGT LRESVMDRVV LHEVSAELLG LLVDFCYTGR VTVTHDNVDL LLKTADLFQF
     PSVKEACCAF LEQRLDVSNC LEIQDFAEAY ACRELAASAR RFVLKNIVEL AKSMDFERLS
     WKRLLEFVSD DGLCVDKEET AYQIAVRWVK ADLQHRLHYW PELLQQVRLP FVRRFYLLAH
     VESDPLVYLS PACLRLVSEA RSFQSYEYDR HDRPGHRMRP RPSTGLAEIL VVVGGCDQDC
     DELVTVDCYN PQTGQWRYLA EFPDHLGGGY SIAALGNDIY VTGGSDGSRL YDCVWRYNSS
     VNEWTEVSPM LKAREYHSSC VLKGQLYVVG SDSTERYDHT IDCWEALPPM PHPMDNCSTT
     ACRGRLYAIG SLTGEDTMAI QCYDAESNRW SLLNSGELPP WSFAPKSVTL NGLIYFVRDD
     SAEVDVYNPQ KNEWDKISPM TQVHVGGSVS ALGGRLYVSG GYDNTFELSD VVEVYDPSSR
     SWSPAGRLPQ PTFWHGSVSI FRQFMPLVQS TFEPIDIPEA NAIHLHRHHR NQALHNHNNN
     VNQNHNQEVN QVH