KLH21_MOUSE
ID KLH21_MOUSE Reviewed; 597 AA.
AC Q3U410; B1AS70; Q3TZ30;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Kelch-like protein 21;
GN Name=Klhl21;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Dendritic cell, and Inner ear;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC ubiquitin-protein ligase complex required for efficient chromosome
CC alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex
CC regulates localization of the chromosomal passenger complex (CPC) from
CC chromosomes to the spindle midzone in anaphase and mediates the
CC ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3
CC ubiquitin ligase complex may not lead to its degradation by the
CC proteasome (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at
CC least composed of CUL3, KLHL21 and RBX1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle {ECO:0000250}.
CC Note=Localizes to the spindle midzone and targets CUL3 to this region.
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE34380.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK154493; BAE32625.1; -; mRNA.
DR EMBL; AK158141; BAE34380.1; ALT_INIT; mRNA.
DR EMBL; AL611927; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466594; EDL14916.1; -; Genomic_DNA.
DR EMBL; BC141158; AAI41159.1; -; mRNA.
DR EMBL; BC141159; AAI41160.1; -; mRNA.
DR CCDS; CCDS18983.1; -.
DR RefSeq; NP_001028524.1; NM_001033352.3.
DR AlphaFoldDB; Q3U410; -.
DR SMR; Q3U410; -.
DR BioGRID; 232459; 16.
DR IntAct; Q3U410; 16.
DR STRING; 10090.ENSMUSP00000095380; -.
DR PhosphoSitePlus; Q3U410; -.
DR PaxDb; Q3U410; -.
DR PRIDE; Q3U410; -.
DR ProteomicsDB; 263622; -.
DR Antibodypedia; 46561; 95 antibodies from 26 providers.
DR Ensembl; ENSMUST00000097773; ENSMUSP00000095380; ENSMUSG00000073700.
DR GeneID; 242785; -.
DR KEGG; mmu:242785; -.
DR UCSC; uc008vyy.1; mouse.
DR CTD; 9903; -.
DR MGI; MGI:1919288; Klhl21.
DR VEuPathDB; HostDB:ENSMUSG00000073700; -.
DR eggNOG; KOG4441; Eukaryota.
DR GeneTree; ENSGT00940000158631; -.
DR HOGENOM; CLU_004253_14_6_1; -.
DR InParanoid; Q3U410; -.
DR OMA; TWSVVGQ; -.
DR OrthoDB; 743142at2759; -.
DR PhylomeDB; Q3U410; -.
DR TreeFam; TF329218; -.
DR Reactome; R-MMU-8951664; Neddylation.
DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 242785; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Klhl21; mouse.
DR PRO; PR:Q3U410; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q3U410; protein.
DR Bgee; ENSMUSG00000073700; Expressed in hindlimb stylopod muscle and 236 other tissues.
DR Genevisible; Q3U410; MM.
DR GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005827; C:polar microtubule; ISS:UniProtKB.
DR GO; GO:0097602; F:cullin family protein binding; ISO:MGI.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISO:MGI.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0035853; P:chromosome passenger complex localization to spindle midzone; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0032465; P:regulation of cytokinesis; ISS:UniProtKB.
DR Gene3D; 2.120.10.80; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR017096; BTB-kelch_protein.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR030577; KLHL21.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR24412:SF255; PTHR24412:SF255; 1.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF01344; Kelch_1; 2.
DR PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00612; Kelch; 5.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Kelch repeat; Mitosis;
KW Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..597
FT /note="Kelch-like protein 21"
FT /id="PRO_0000326128"
FT DOMAIN 35..103
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 138..239
FT /note="BACK"
FT REPEAT 287..335
FT /note="Kelch 1"
FT REPEAT 336..382
FT /note="Kelch 2"
FT REPEAT 384..422
FT /note="Kelch 3"
FT REPEAT 423..470
FT /note="Kelch 4"
FT REPEAT 472..512
FT /note="Kelch 5"
FT REPEAT 513..560
FT /note="Kelch 6"
FT REGION 570..597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 382
FT /note="G -> S (in Ref. 1; BAE34380)"
FT /evidence="ECO:0000305"
FT CONFLICT 438
FT /note="I -> V (in Ref. 1; BAE34380)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 597 AA; 66547 MW; 1EE233F7F8D6F5DD CRC64;
MERPAPLAVL PFSDPAHALS LLRGLSQLRA ERKFLDVTLE AAGGRDFPAH RAVLAAASPY
FRAMFAGQLR ESRAERVRLH GVPPDMLQLL LDFSYTGRVA VSGDNAEPLL RAADLLQFPA
VKEACGAFLQ QQLDLANCLD MQDFAEAFSC SGLASAAQRF ILRHVGELGA EQLERLPLAR
LLRYLRDDGL CVPKEEAAYQ LALRWVRADP PRRAPHWPQL LEAVRLPFVR RFYLLAHVEA
EPLVARCPPC LRLLREARDF QAARYDRHDR GPCPRMRPRP STGLAEILVL VGGCDQDCDE
LVTVDCYNPQ TGQWRYLAEF PDHLGGGYSI VALGNDIYVT GGSDGSRLYD CVWRYNSSVN
EWTEVAPMLK AREYHSSSVL DGLLYVVAAD STERYDHATD SWEALQPMTY PMDNCSTTAC
RGRLYAIGSL AGKETMVIQC YDPDTDLWSL VNCGQLPPWS FAPKTVTLNG LMYFVRDDSA
EVDVYNPTKD EWDKIPSMNQ VHVGGSLAVL GGKLYVSGGY DNTFELSDVV EAYDPETRAW
SVVGRLPEPT FWHGSVSIFR QFMPQTPAGG RGFELNSGSN DVDAGYHRLP QNPEELH
