KLH21_RAT
ID KLH21_RAT Reviewed; 597 AA.
AC D4A2K4;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Kelch-like protein 21;
GN Name=Klhl21;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Substrate-specific adapter of BCR (BTB-CUL3-RBX1) E3
CC ubiquitin-protein ligase complex required for efficient chromosome
CC alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex
CC regulates localization of the chromosomal passenger complex (CPC) from
CC chromosomes to the spindle midzone in anaphase and mediates the
CC ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3
CC ubiquitin ligase complex may not lead to its degradation by the
CC proteasome (By similarity). {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at
CC least composed of CUL3, KLHL21 and RBX1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle {ECO:0000250}.
CC Note=Localizes to the spindle midzone and targets CUL3 to this region.
CC {ECO:0000250}.
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DR EMBL; CH473968; EDL81211.1; -; Genomic_DNA.
DR RefSeq; NP_001101466.1; NM_001107996.1.
DR AlphaFoldDB; D4A2K4; -.
DR SMR; D4A2K4; -.
DR STRING; 10116.ENSRNOP00000004508; -.
DR PaxDb; D4A2K4; -.
DR Ensembl; ENSRNOT00000004508; ENSRNOP00000004508; ENSRNOG00000003334.
DR GeneID; 313743; -.
DR KEGG; rno:313743; -.
DR UCSC; RGD:1305863; rat.
DR CTD; 9903; -.
DR RGD; 1305863; Klhl21.
DR eggNOG; KOG4441; Eukaryota.
DR GeneTree; ENSGT00940000158631; -.
DR HOGENOM; CLU_004253_14_6_1; -.
DR InParanoid; D4A2K4; -.
DR OMA; TWSVVGQ; -.
DR OrthoDB; 743142at2759; -.
DR PhylomeDB; D4A2K4; -.
DR TreeFam; TF329218; -.
DR Reactome; R-RNO-8951664; Neddylation.
DR Reactome; R-RNO-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR UniPathway; UPA00143; -.
DR PRO; PR:D4A2K4; -.
DR Proteomes; UP000002494; Chromosome 5.
DR Proteomes; UP000234681; Chromosome 5.
DR Bgee; ENSRNOG00000003334; Expressed in skeletal muscle tissue and 19 other tissues.
DR Genevisible; D4A2K4; RN.
DR GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005827; C:polar microtubule; ISS:UniProtKB.
DR GO; GO:0097602; F:cullin family protein binding; ISO:RGD.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:Ensembl.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0035853; P:chromosome passenger complex localization to spindle midzone; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0032465; P:regulation of cytokinesis; ISS:UniProtKB.
DR Gene3D; 2.120.10.80; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR017096; BTB-kelch_protein.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR030577; KLHL21.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR24412:SF255; PTHR24412:SF255; 1.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF01344; Kelch_1; 2.
DR PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00612; Kelch; 5.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Kelch repeat; Mitosis;
KW Reference proteome; Repeat; Ubl conjugation pathway.
FT CHAIN 1..597
FT /note="Kelch-like protein 21"
FT /id="PRO_0000396631"
FT DOMAIN 35..103
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 138..239
FT /note="BACK"
FT REPEAT 287..335
FT /note="Kelch 1"
FT REPEAT 336..382
FT /note="Kelch 2"
FT REPEAT 384..422
FT /note="Kelch 3"
FT REPEAT 423..470
FT /note="Kelch 4"
FT REPEAT 472..512
FT /note="Kelch 5"
FT REPEAT 513..560
FT /note="Kelch 6"
FT REGION 570..597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 597 AA; 66448 MW; BB40E784787D3124 CRC64;
MERPAPLAVL PFSDPAHALS LLRGLSQLRA ERKFLDVTLE AAGGRDFPAH RAVLAAASPY
FRAMFAGQLR ESRAERVRLH GVPPDMLQLL LDFSYTGRVA VSGDNAEPLL RAADLLQFPA
VKEACGAFLQ QQLDLANCLD MQDFAEAFSC SGLASAAQRF ILRHVGELGA EQLERLPLAR
LLRYLRDDGL CVPKEEAAYQ LALRWVRADP PRRAAHWPQL LEAVRLPFVR RFYLLAHVEA
EPLVARCPPC LRLLREARDF QAARYDRHDR GPCPRMRPRP STGLAEILVL VGGCDQDCDE
LVTVDCYNPQ TGQWRYLAEF PDHLGGGYSI VALGNDIYVT GGSDGSRLYD CVWRYNSSVN
EWTEVAPMLK AREYHSSSVL NGLLYVVAAD STERYDHATD SWEALQPMTY PMDNCSTTAC
RGRLYAIGSL AGKETMVIQC YDPDTDLWSM VNCGQLPPWS FAPKTVTLNG LMYFVRDDSA
EVDVYNPTKD EWDKIPSMNQ VHVGGSLAAL GGKLYVSGGY DNTFELSDVV EAYDPETRAW
SVVGRLPEPT FWHGSVSIFR QFMPQTPAGG RGFELNSGSS DVDAGHHRLP QNPEELQ
