ID   KLH21_XENLA             Reviewed;         615 AA.
AC   Q5U575;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Kelch-like protein 21;
GN   Name=klhl21;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Substrate-specific adapter of BCR (BTB-CUL3-RBX1) E3
CC       ubiquitin-protein ligase complex required for efficient chromosome
CC       alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex
CC       regulates localization of the chromosomal passenger complex (CPC) from
CC       chromosomes to the spindle midzone in anaphase and mediates the
CC       ubiquitination of AURKB (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Component of the BCR(KLHL21) E3 ubiquitin ligase complex, at
CC       least composed of cul3, klhl21 and rbx1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, spindle {ECO:0000250}.
CC       Note=Localizes to the spindle midzone and targets CUL3 to this region.
CC       {ECO:0000250}.
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DR   EMBL; BC084809; AAH84809.1; -; mRNA.
DR   RefSeq; NP_001088480.1; NM_001095011.1.
DR   RefSeq; XP_018080080.1; XM_018224591.1.
DR   RefSeq; XP_018080081.1; XM_018224592.1.
DR   RefSeq; XP_018080082.1; XM_018224593.1.
DR   AlphaFoldDB; Q5U575; -.
DR   SMR; Q5U575; -.
DR   DNASU; 495346; -.
DR   GeneID; 495346; -.
DR   KEGG; xla:495346; -.
DR   CTD; 495346; -.
DR   Xenbase; XB-GENE-5802704; klhl21.L.
DR   OMA; VMEREVM; -.
DR   OrthoDB; 743142at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000186698; Chromosome 7L.
DR   Bgee; 495346; Expressed in muscle tissue and 19 other tissues.
DR   GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005827; C:polar microtubule; ISS:UniProtKB.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0035853; P:chromosome passenger complex localization to spindle midzone; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0032465; P:regulation of cytokinesis; ISS:UniProtKB.
DR   Gene3D; 2.120.10.80; -; 1.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR011705; BACK.
DR   InterPro; IPR017096; BTB-kelch_protein.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR030577; KLHL21.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   PANTHER; PTHR24412:SF255; PTHR24412:SF255; 1.
DR   Pfam; PF07707; BACK; 1.
DR   Pfam; PF00651; BTB; 1.
DR   Pfam; PF01344; Kelch_1; 2.
DR   PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR   SMART; SM00875; BACK; 1.
DR   SMART; SM00225; BTB; 1.
DR   SMART; SM00612; Kelch; 5.
DR   SUPFAM; SSF117281; SSF117281; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Kelch repeat; Mitosis;
KW   Reference proteome; Repeat; Ubl conjugation pathway.
FT   CHAIN           1..615
FT                   /note="Kelch-like protein 21"
FT                   /id="PRO_0000396633"
FT   DOMAIN          46..113
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT   DOMAIN          148..249
FT                   /note="BACK"
FT   REPEAT          297..345
FT                   /note="Kelch 1"
FT   REPEAT          346..392
FT                   /note="Kelch 2"
FT   REPEAT          394..432
FT                   /note="Kelch 3"
FT   REPEAT          433..480
FT                   /note="Kelch 4"
FT   REPEAT          482..522
FT                   /note="Kelch 5"
FT   REPEAT          523..570
FT                   /note="Kelch 6"
SQ   SEQUENCE   615 AA;  70129 MW;  A8CEFE9E146007F7 CRC64;
     MESPINTSPD SVGIPAPEAA LPFYDASHAL HLLRGINELR SEQKFFDMTV SAGGQDFPCH
     RTVLAAASNY FRAMFAGRLR ESHADRVELH DVSGPILSLL LDFCYTGRVT VTLENVEPLL
     QAADLFQFPS VKQACCAYLE QQLDVSNCLE IQDFAEAYAC QGLAESTKRF ILGHIAQLAQ
     AHEVERLTFK RMLEYISDDR LCVDKEESAF QIALQWVKAD PKHRQYRWPE LFQYLRLPFI
     RRFYLLAYVE SDPLVFRSPY CLQLIREARA FQSSEYDRHD WLCHRMKPRP STGLAEILVV
     VGGCDQECDE LVTVDCYNPQ TGQWRYLAEF PDHLGGGYSI AALGNDIYVT GGSDGSRLYD
     CVWRYNSSVN EWTEVSPMLK AREYHSSTVL NGLLYVIASD STERYDPTMD TWESLHPMLY
     PMDNCSTTSC RKKLYAIGSL EGQESMVMQC YNPDTNVWAM VNCGQLPPWS FAPKTVTLNG
     LIYFVRDDSP EVDVYNPSKN EWDKIPPMIQ VHVGGSLAAL GGKLYVSGGY DNTFELSDVV
     EAFDPETRSW SLMGRLPQPT FWQGSVSIFR QFMPQTQQSL DGVPSDNTNN ERNIINLNRR
     RHHLHNHNFN ELHRR