ID   KLH25_XENTR             Reviewed;         589 AA.
AC   Q0D2A9;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Kelch-like protein 25;
DE   AltName: Full=Ectoderm-neural cortex protein 2;
DE            Short=ENC-2;
GN   Name=klhl25; Synonyms=enc2;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=N6; TISSUE=Ovary;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC       ubiquitin ligase complex required for translational homeostasis. The
CC       BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination
CC       of hypophosphorylated eif4ebp1 (4E-BP1) (By similarity). {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Component of the BCR(KLHL25) E3 ubiquitin ligase complex, at
CC       least composed of cul3, klhl25 and rbx1. {ECO:0000250}.
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DR   EMBL; BC122041; AAI22042.1; -; mRNA.
DR   RefSeq; NP_001072541.1; NM_001079073.1.
DR   RefSeq; XP_012814705.1; XM_012959251.2.
DR   RefSeq; XP_012814706.1; XM_012959252.2.
DR   RefSeq; XP_012814709.1; XM_012959255.2.
DR   RefSeq; XP_017947662.1; XM_018092173.1.
DR   AlphaFoldDB; Q0D2A9; -.
DR   SMR; Q0D2A9; -.
DR   STRING; 8364.ENSXETP00000051270; -.
DR   PaxDb; Q0D2A9; -.
DR   DNASU; 779996; -.
DR   GeneID; 779996; -.
DR   KEGG; xtr:779996; -.
DR   CTD; 64410; -.
DR   Xenbase; XB-GENE-964636; klhl25.
DR   eggNOG; KOG4441; Eukaryota.
DR   HOGENOM; CLU_004253_14_3_1; -.
DR   InParanoid; Q0D2A9; -.
DR   OMA; HIVFNAV; -.
DR   OrthoDB; 709680at2759; -.
DR   PhylomeDB; Q0D2A9; -.
DR   TreeFam; TF329218; -.
DR   Reactome; R-XTR-8951664; Neddylation.
DR   Reactome; R-XTR-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000008143; Chromosome 3.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000007622; Expressed in ovary and 13 other tissues.
DR   GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0006446; P:regulation of translational initiation; ISS:UniProtKB.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR   Gene3D; 2.120.10.80; -; 2.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR011705; BACK.
DR   InterPro; IPR017096; BTB-kelch_protein.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR030565; KLHL25.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   PANTHER; PTHR24410:SF10; PTHR24410:SF10; 1.
DR   Pfam; PF07707; BACK; 1.
DR   Pfam; PF00651; BTB; 1.
DR   Pfam; PF01344; Kelch_1; 5.
DR   PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR   SMART; SM00875; BACK; 1.
DR   SMART; SM00225; BTB; 1.
DR   SMART; SM00612; Kelch; 6.
DR   SUPFAM; SSF117281; SSF117281; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
PE   2: Evidence at transcript level;
KW   Kelch repeat; Reference proteome; Repeat; Translation regulation;
KW   Ubl conjugation pathway.
FT   CHAIN           1..589
FT                   /note="Kelch-like protein 25"
FT                   /id="PRO_0000272312"
FT   DOMAIN          46..114
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT   DOMAIN          149..250
FT                   /note="BACK"
FT   REPEAT          296..340
FT                   /note="Kelch 1"
FT   REPEAT          341..388
FT                   /note="Kelch 2"
FT   REPEAT          389..444
FT                   /note="Kelch 3"
FT   REPEAT          446..492
FT                   /note="Kelch 4"
FT   REPEAT          493..538
FT                   /note="Kelch 5"
FT   REPEAT          539..585
FT                   /note="Kelch 6"
SQ   SEQUENCE   589 AA;  65955 MW;  8D21A496F513BEC9 CRC64;
     MSVTVHENRK SRTSTGSMNI SLYHKLSHSD CVLNHLNTMR KQRLFTDVTL WAGNRSFPCH
     RAVLAACSQY FEAMFSNGLR ESLDNTVNFH DSLHPEVLEL LLDFAYSSKI IINEENAESL
     LEAGDMLQFH DIRDAACEFL EKNLYPSNCL GMMILSDAHQ CQRLYELSLR TCLSNFATLH
     NTEDFCSLSK DMVLDLISSD ELEIEDEQVV FNSVLHWVKE DLDKRKDYFP ELLRKVRLAL
     LPSELLKEAV VCEDLIIADE RSKLIMDEAV VCKKKILQND GVVTSLCAKP RKAGHTLLIL
     GGQTFMCDKI YQVDHKAKEI IPKADLPSPR KEFSACAIGC KVYVTGGRGS ENGVSKDVWV
     YDTINEEWSK SAPMLIARFG HGSAELENCL YVVGGHTAVA GVFPASPSVS LKQVEKYDPL
     TNKWTMMAPL RDGVSNAAVV SAKLKLFAFG GTSIHRDRVS KVQCYDPDEN RWSIKAECPQ
     PWRYTAAAVL GSQIFIMGGD TEFTAASAYR FDCETNQWTR IGDMTAKRMS CHALASGNKV
     YVVGGYFGTQ RCKTLDCYDP TSDSWNSITS VPYSLIPTAF VSTWKHLPA