KLH31_MOUSE
ID KLH31_MOUSE Reviewed; 634 AA.
AC Q8BWA5; Q148Q5;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Kelch-like protein 31;
DE AltName: Full=Kelch repeat and BTB domain-containing protein 1;
DE AltName: Full=Kelch-like protein KLHL;
GN Name=Klhl31; Synonyms=Kbtbd1, Klhl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP IDENTIFICATION.
RX PubMed=15302408; DOI=10.1016/j.gene.2004.05.016;
RA Wu Y.L., Gong Z.;
RT "A novel zebrafish kelchlike gene klhl and its human ortholog KLHL display
RT conserved expression patterns in skeletal and cardiac muscles.";
RL Gene 338:75-83(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP CLEAVAGE OF INITIATOR METHIONINE, AND METHYLATION AT ALA-2.
RX PubMed=20668449; DOI=10.1038/nature09343;
RA Tooley C.E., Petkowski J.J., Muratore-Schroeder T.L., Balsbaugh J.L.,
RA Shabanowitz J., Sabat M., Minor W., Hunt D.F., Macara I.G.;
RT "NRMT is an alpha-N-methyltransferase that methylates RCC1 and
RT retinoblastoma protein.";
RL Nature 466:1125-1128(2010).
CC -!- FUNCTION: Transcriptional repressor in MAPK/JNK signaling pathway to
CC regulate cellular functions. Overexpression inhibits the
CC transcriptional activities of both the TPA-response element (TRE) and
CC serum response element (SRE) (By similarity). {ECO:0000250}.
CC -!- PTM: N-terminus is methylated by METTL11A/NTM1.
CC {ECO:0000269|PubMed:20668449}.
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DR EMBL; AK053085; BAC35261.1; -; mRNA.
DR CCDS; CCDS23352.1; -.
DR RefSeq; NP_766513.2; NM_172925.2.
DR AlphaFoldDB; Q8BWA5; -.
DR SMR; Q8BWA5; -.
DR STRING; 10090.ENSMUSP00000059643; -.
DR iPTMnet; Q8BWA5; -.
DR PhosphoSitePlus; Q8BWA5; -.
DR MaxQB; Q8BWA5; -.
DR PaxDb; Q8BWA5; -.
DR PRIDE; Q8BWA5; -.
DR ProteomicsDB; 263628; -.
DR DNASU; 244923; -.
DR GeneID; 244923; -.
DR KEGG; mmu:244923; -.
DR CTD; 401265; -.
DR MGI; MGI:3045305; Klhl31.
DR eggNOG; KOG4441; Eukaryota.
DR InParanoid; Q8BWA5; -.
DR OrthoDB; 250404at2759; -.
DR PhylomeDB; Q8BWA5; -.
DR BioGRID-ORCS; 244923; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q8BWA5; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8BWA5; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0046329; P:negative regulation of JNK cascade; ISS:UniProtKB.
DR GO; GO:0001933; P:negative regulation of protein phosphorylation; ISS:UniProtKB.
DR Gene3D; 2.120.10.80; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR017096; BTB-kelch_protein.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR030604; KLHL31.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR45632:SF17; PTHR45632:SF17; 1.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF01344; Kelch_1; 3.
DR PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00612; Kelch; 6.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW Kelch repeat; Methylation; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:20668449"
FT CHAIN 2..634
FT /note="Kelch-like protein 31"
FT /id="PRO_0000271036"
FT DOMAIN 73..137
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 172..273
FT /note="BACK"
FT REPEAT 317..365
FT /note="Kelch 1"
FT REPEAT 366..419
FT /note="Kelch 2"
FT REPEAT 420..466
FT /note="Kelch 3"
FT REPEAT 468..513
FT /note="Kelch 4"
FT REPEAT 515..565
FT /note="Kelch 5"
FT REPEAT 566..614
FT /note="Kelch 6"
FT MOD_RES 2
FT /note="N,N,N-trimethylalanine; by NTM1"
FT /evidence="ECO:0000269|PubMed:20668449"
SQ SEQUENCE 634 AA; 70181 MW; CDAA6821191AAF5B CRC64;
MAPKKKTIKK NKAEINEMTI IVEDSPLSKL NALNGLLEGS NSLSCVSSEL TDTSYGPNLL
EGLSKMRQES FLCDLVIGTK TKSFDVHKSV MASCSEYFYN ILKNDPSTKR VDLNDIAPLG
LATVIAYAYT GKLTLSLYTI GSIISAAVYL QIHTLVKMCS DFLIREISVE NCMYVVNIAE
TYSLKNAKAT AQKFIRDNFI EFAESEQFMK LTFEQINELL VDDDLQLPSE LVAFQIAMKW
LEFDQKRVKH AADLLSNIRF GTISAQDLVN YVQTVPRMMQ DADCHKLLVD AMNYHLLPYH
QNTLQSRRTR IRGGCRVLIT VGGRPGLTEK SLSRDILYRD PENGWSKLTE MPAKSFNQCV
AVMDGFLYVA GGEDQNDARN QAKHAVSNFC RYDPRFNTWI HLGSMNQKRT HFSLSVFNGL
LYAVGGRNSE GSLASLECYV PSTNQWQPKA PLEVARCCHA SAVADGRVIV TGGYIGSAYS
RSVCAYDPAL DAWQELPQLS TPRGWHCAVA LGDRLYVMGG SQLGPRGERV DVLTVESFSP
AARQWSFVAP LPVGVSTAGV SALHGRAYLL GGWNEGDKKY KKCIQCFNPE LNEWTEDDEL
PEATVGVSCC TLAMPNSVSR ESRASSVSSV PVSI
