KLH40_HUMAN
ID KLH40_HUMAN Reviewed; 621 AA.
AC Q2TBA0; Q86SI1; Q96MR2;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Kelch-like protein 40 {ECO:0000305};
DE AltName: Full=Kelch repeat and BTB domain-containing protein 5;
DE AltName: Full=Sarcosynapsin {ECO:0000303|Ref.1};
GN Name=KLHL40 {ECO:0000312|HGNC:HGNC:30372};
GN Synonyms=KBTBD5, SRYP {ECO:0000303|Ref.1};
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Heart;
RA Goudou D., Bitoun M., Rieger F., Perin J.-P., Alliel P.M.;
RT "Structure, gene organization and expression analysis of human
RT sarcosynapsin.";
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT SER-345.
RC TISSUE=Tongue;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS SER-345
RP AND ARG-617.
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) OF 314-621.
RX PubMed=23349464; DOI=10.1074/jbc.m112.437996;
RA Canning P., Cooper C.D., Krojer T., Murray J.W., Pike A.C., Chaikuad A.,
RA Keates T., Thangaratnarajah C., Hojzan V., Marsden B.D., Gileadi O.,
RA Knapp S., von Delft F., Bullock A.N.;
RT "Structural basis for Cul3 assembly with the BTB-Kelch family of E3
RT ubiquitin ligases.";
RL J. Biol. Chem. 288:7803-7814(2013).
RN [5]
RP VARIANTS NEM8 HIS-34; PRO-86; GLU-194; LEU-201; LEU-311; LEU-397; ARG-455;
RP CYS-469; PRO-506; LYS-528; PRO-538 AND LYS-588, FUNCTION, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23746549; DOI=10.1016/j.ajhg.2013.05.004;
RA Ravenscroft G., Miyatake S., Lehtokari V.L., Todd E.J., Vornanen P.,
RA Yau K.S., Hayashi Y.K., Miyake N., Tsurusaki Y., Doi H., Saitsu H.,
RA Osaka H., Yamashita S., Ohya T., Sakamoto Y., Koshimizu E., Imamura S.,
RA Yamashita M., Ogata K., Shiina M., Bryson-Richardson R.J., Vaz R.,
RA Ceyhan O., Brownstein C.A., Swanson L.C., Monnot S., Romero N.B.,
RA Amthor H., Kresoje N., Sivadorai P., Kiraly-Borri C., Haliloglu G.,
RA Talim B., Orhan D., Kale G., Charles A.K., Fabian V.A., Davis M.R.,
RA Lammens M., Sewry C.A., Manzur A., Muntoni F., Clarke N.F., North K.N.,
RA Bertini E., Nevo Y., Willichowski E., Silberg I.E., Topaloglu H.,
RA Beggs A.H., Allcock R.J., Nishino I., Wallgren-Pettersson C., Matsumoto N.,
RA Laing N.G.;
RT "Mutations in KLHL40 are a frequent cause of severe autosomal-recessive
RT nemaline myopathy.";
RL Am. J. Hum. Genet. 93:6-18(2013).
RN [6]
RP VARIANT NEM8 PRO-505.
RX PubMed=26754003; DOI=10.1007/s00415-015-8015-x;
RA Natera-de Benito D., Nascimento A., Abicht A., Ortez C., Jou C.,
RA Mueller J.S., Evangelista T., Toepf A., Thompson R., Jimenez-Mallebrera C.,
RA Colomer J., Lochmueller H.;
RT "KLHL40-related nemaline myopathy with a sustained, positive response to
RT treatment with acetylcholinesterase inhibitors.";
RL J. Neurol. 263:517-523(2016).
RN [7]
RP VARIANT NEM8 CYS-500.
RX PubMed=27528495; DOI=10.1016/j.nmd.2016.07.011;
RA Seferian A.M., Malfatti E., Bosson C., Pelletier L., Taytard J., Forin V.,
RA Gidaro T., Gargaun E., Carlier P., Faure J., Romero N.B., Rendu J.,
RA Servais L.;
RT "Mild clinical presentation in KLHL40-related nemaline myopathy (NEM 8).";
RL Neuromuscul. Disord. 26:712-716(2016).
RN [8]
RP VARIANTS NEM8 201-TRP--MET-621 DEL AND PRO-506.
RX PubMed=27762439; DOI=10.1002/pd.4949;
RA Chen T.H., Tian X., Kuo P.L., Pan H.P., Wong L.C., Jong Y.J.;
RT "Identification of KLHL40 mutations by targeted next-generation sequencing
RT facilitated a prenatal diagnosis in a family with three consecutive
RT affected fetuses with fetal akinesia deformation sequence.";
RL Prenat. Diagn. 36:1135-1138(2016).
CC -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC ubiquitin ligase complex that acts as a key regulator of skeletal
CC muscle development (PubMed:23746549). The BCR(KLHL40) complex acts by
CC mediating ubiquitination and degradation of TFDP1, thereby regulating
CC the activity of the E2F:DP transcription factor complex (By
CC similarity). Promotes stabilization of LMOD3 by acting as a negative
CC regulator of LMOD3 ubiquitination; the molecular process by which it
CC negatively regulates ubiquitination of LMOD3 is however unclear (By
CC similarity). {ECO:0000250|UniProtKB:Q9D783,
CC ECO:0000269|PubMed:23746549}.
CC -!- SUBUNIT: Component of the BCR(KLHL40) E3 ubiquitin ligase complex, at
CC least composed of CUL3, KLHL40 and RBX1. Interacts with LMOD3.
CC {ECO:0000250|UniProtKB:Q9D783}.
CC -!- INTERACTION:
CC Q2TBA0; A1L4K1: FSD2; NbExp=3; IntAct=EBI-7851314, EBI-5661036;
CC Q2TBA0; Q7L590-2: MCM10; NbExp=4; IntAct=EBI-7851314, EBI-10233517;
CC Q2TBA0; Q8IYX7: SAXO1; NbExp=3; IntAct=EBI-7851314, EBI-3957636;
CC Q2TBA0; Q5VWN6: TASOR2; NbExp=3; IntAct=EBI-7851314, EBI-745958;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9D783}.
CC Cytoplasm, myofibril, sarcomere, A band {ECO:0000269|PubMed:23746549}.
CC Cytoplasm, myofibril, sarcomere, I band {ECO:0000250|UniProtKB:Q9D783}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q2TBA0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q2TBA0-2; Sequence=VSP_022681, VSP_022682;
CC -!- TISSUE SPECIFICITY: Highly expressed in fetal (19, 23 and 31 weeks of
CC gestation) and adult skeletal muscle; expression levels tend to be
CC higher in fetal compared to postnatal muscles (at protein level). Also
CC expressed in fetal and adult heart. {ECO:0000269|PubMed:23746549}.
CC -!- DISEASE: Nemaline myopathy 8 (NEM8) [MIM:615348]: A severe form of
CC nemaline myopathy. Nemaline myopathies are muscular disorders
CC characterized by muscle weakness of varying severity and onset, and
CC abnormal thread-like or rod-shaped structures in muscle fibers on
CC histologic examination. NEM8 is characterized by fetal akinesia or
CC hypokinesia, followed by contractures, fractures, respiratory failure,
CC and swallowing difficulties apparent at birth. Most patients die in
CC infancy. Skeletal muscle biopsy shows numerous small nemaline bodies,
CC often with no normal myofibrils. {ECO:0000269|PubMed:23746549,
CC ECO:0000269|PubMed:26754003, ECO:0000269|PubMed:27528495,
CC ECO:0000269|PubMed:27762439}. Note=The disease is caused by variants
CC affecting the gene represented in this entry.
CC -!- SIMILARITY: Belongs to the KLHL40 family. {ECO:0000305}.
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DR EMBL; AY177390; AAO06908.1; -; mRNA.
DR EMBL; AY176040; AAO22141.1; -; mRNA.
DR EMBL; AK056577; BAB71222.1; -; mRNA.
DR EMBL; BC110491; AAI10492.1; -; mRNA.
DR CCDS; CCDS2703.1; -. [Q2TBA0-1]
DR RefSeq; NP_689606.2; NM_152393.3. [Q2TBA0-1]
DR PDB; 4ASC; X-ray; 1.78 A; A=314-621.
DR PDBsum; 4ASC; -.
DR AlphaFoldDB; Q2TBA0; -.
DR SMR; Q2TBA0; -.
DR BioGRID; 126278; 70.
DR IntAct; Q2TBA0; 35.
DR MINT; Q2TBA0; -.
DR STRING; 9606.ENSP00000287777; -.
DR iPTMnet; Q2TBA0; -.
DR PhosphoSitePlus; Q2TBA0; -.
DR BioMuta; KLHL40; -.
DR DMDM; 125950763; -.
DR MassIVE; Q2TBA0; -.
DR PaxDb; Q2TBA0; -.
DR PeptideAtlas; Q2TBA0; -.
DR PRIDE; Q2TBA0; -.
DR ProteomicsDB; 61486; -. [Q2TBA0-1]
DR ProteomicsDB; 61487; -. [Q2TBA0-2]
DR Antibodypedia; 12393; 112 antibodies from 19 providers.
DR DNASU; 131377; -.
DR Ensembl; ENST00000287777.5; ENSP00000287777.4; ENSG00000157119.12. [Q2TBA0-1]
DR GeneID; 131377; -.
DR KEGG; hsa:131377; -.
DR MANE-Select; ENST00000287777.5; ENSP00000287777.4; NM_152393.4; NP_689606.2.
DR UCSC; uc003clv.2; human. [Q2TBA0-1]
DR CTD; 131377; -.
DR DisGeNET; 131377; -.
DR GeneCards; KLHL40; -.
DR HGNC; HGNC:30372; KLHL40.
DR HPA; ENSG00000157119; Group enriched (skeletal muscle, tongue).
DR MalaCards; KLHL40; -.
DR MIM; 615340; gene.
DR MIM; 615348; phenotype.
DR neXtProt; NX_Q2TBA0; -.
DR OpenTargets; ENSG00000157119; -.
DR Orphanet; 171430; Severe congenital nemaline myopathy.
DR PharmGKB; PA134908127; -.
DR VEuPathDB; HostDB:ENSG00000157119; -.
DR eggNOG; KOG4441; Eukaryota.
DR GeneTree; ENSGT00940000156360; -.
DR HOGENOM; CLU_004253_14_4_1; -.
DR InParanoid; Q2TBA0; -.
DR OMA; PYVVYGH; -.
DR PhylomeDB; Q2TBA0; -.
DR TreeFam; TF351653; -.
DR PathwayCommons; Q2TBA0; -.
DR SignaLink; Q2TBA0; -.
DR BioGRID-ORCS; 131377; 13 hits in 1102 CRISPR screens.
DR ChiTaRS; KLHL40; human.
DR GenomeRNAi; 131377; -.
DR Pharos; Q2TBA0; Tbio.
DR PRO; PR:Q2TBA0; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q2TBA0; protein.
DR Bgee; ENSG00000157119; Expressed in gastrocnemius and 83 other tissues.
DR Genevisible; Q2TBA0; HS.
DR GO; GO:0031672; C:A band; IBA:GO_Central.
DR GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0031674; C:I band; IBA:GO_Central.
DR GO; GO:0032435; P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0031397; P:negative regulation of protein ubiquitination; IBA:GO_Central.
DR GO; GO:0032436; P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR GO; GO:0031398; P:positive regulation of protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0048741; P:skeletal muscle fiber development; ISS:UniProtKB.
DR GO; GO:0098528; P:skeletal muscle fiber differentiation; ISS:UniProtKB.
DR Gene3D; 2.120.10.80; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR017096; BTB-kelch_protein.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR030607; KLHL40.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR24412:SF22; PTHR24412:SF22; 1.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF01344; Kelch_1; 3.
DR PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00612; Kelch; 4.
DR SUPFAM; SSF117281; SSF117281; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cytoplasm; Developmental protein;
KW Disease variant; Kelch repeat; Nemaline myopathy; Reference proteome;
KW Repeat; Ubl conjugation pathway.
FT CHAIN 1..621
FT /note="Kelch-like protein 40"
FT /id="PRO_0000274235"
FT DOMAIN 33..98
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 133..239
FT /note="BACK"
FT REPEAT 360..412
FT /note="Kelch 1"
FT REPEAT 413..462
FT /note="Kelch 2"
FT REPEAT 463..510
FT /note="Kelch 3"
FT REPEAT 512..557
FT /note="Kelch 4"
FT REPEAT 559..613
FT /note="Kelch 5"
FT REGION 265..295
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 272..295
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 444..604
FT /note="ESDPLPYVVYGHTVLSHMDLVYVIGGKGSDRKCLNKMCVYDPKKFEWKELAP
FT MQTARSLFGATVHDGRIIVAAGVTDTGLTSSAEVYSITDNKWAPFEAFPQERSSLSLVS
FT LVGTLYAIGGFATLETESGELVPTELNDIWRYNEEEKKWEGVLREIAYAA -> HRHRA
FT DQFCRSVQHHRQQVGTLRGLPTGA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_022681"
FT VAR_SEQ 605..621
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_022682"
FT VARIANT 34
FT /note="D -> H (in NEM8; dbSNP:rs778565563)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069836"
FT VARIANT 86
FT /note="L -> P (in NEM8)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069837"
FT VARIANT 194
FT /note="V -> E (in NEM8)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069838"
FT VARIANT 201..621
FT /note="Missing (in NEM8)"
FT /evidence="ECO:0000269|PubMed:27762439"
FT /id="VAR_077950"
FT VARIANT 201
FT /note="W -> L (in NEM8; dbSNP:rs397509420)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069839"
FT VARIANT 311
FT /note="R -> L (in NEM8; dbSNP:rs774887948)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069840"
FT VARIANT 345
FT /note="N -> S (in dbSNP:rs6805421)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_030214"
FT VARIANT 397
FT /note="P -> L (in NEM8; dbSNP:rs773649133)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069841"
FT VARIANT 455
FT /note="H -> R (in NEM8; dbSNP:rs770866675)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069842"
FT VARIANT 469
FT /note="G -> C (in NEM8; dbSNP:rs367579275)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069843"
FT VARIANT 500
FT /note="R -> C (in NEM8; unknown pathological significance;
FT dbSNP:rs758188096)"
FT /evidence="ECO:0000269|PubMed:27528495"
FT /id="VAR_077951"
FT VARIANT 505
FT /note="A -> P (in NEM8)"
FT /evidence="ECO:0000269|PubMed:26754003"
FT /id="VAR_077952"
FT VARIANT 506
FT /note="T -> P (in NEM8; dbSNP:rs778022582)"
FT /evidence="ECO:0000269|PubMed:23746549,
FT ECO:0000269|PubMed:27762439"
FT /id="VAR_069844"
FT VARIANT 528
FT /note="E -> K (in NEM8; dbSNP:rs397509419)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069845"
FT VARIANT 538
FT /note="A -> P (in NEM8; dbSNP:rs397509421)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069846"
FT VARIANT 588
FT /note="E -> K (in NEM8; dbSNP:rs201856772)"
FT /evidence="ECO:0000269|PubMed:23746549"
FT /id="VAR_069847"
FT VARIANT 617
FT /note="C -> R (in dbSNP:rs123509)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_030215"
FT STRAND 317..324
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 327..332
FT /evidence="ECO:0007829|PDB:4ASC"
FT TURN 333..336
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 337..342
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 349..355
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 361..369
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 374..376
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 378..386
FT /evidence="ECO:0007829|PDB:4ASC"
FT TURN 387..390
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 391..394
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 398..400
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 402..404
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 406..410
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 413..417
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 420..423
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 432..436
FT /evidence="ECO:0007829|PDB:4ASC"
FT TURN 437..440
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 441..444
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 456..460
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 463..467
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 480..484
FT /evidence="ECO:0007829|PDB:4ASC"
FT TURN 485..488
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 489..492
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 504..508
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 511..518
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 520..531
FT /evidence="ECO:0007829|PDB:4ASC"
FT TURN 532..535
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 536..540
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 551..555
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 558..569
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 575..587
FT /evidence="ECO:0007829|PDB:4ASC"
FT TURN 588..591
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 592..598
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 607..613
FT /evidence="ECO:0007829|PDB:4ASC"
FT HELIX 615..617
FT /evidence="ECO:0007829|PDB:4ASC"
FT STRAND 618..620
FT /evidence="ECO:0007829|PDB:4ASC"
SQ SEQUENCE 621 AA; 69257 MW; 53492478F08A5A2D CRC64;
MALGLEQAEE QRLYQQTLLQ DGLKDMLDHG KFLDCVVRAG EREFPCHRLV LAACSPYFRA
RFLAEPERAG ELHLEEVSPD VVAQVLHYLY TSEIALDEAS VQDLFAAAHR FQIPSIFTIC
VSFLQKRLCL SNCLAVFRLG LLLDCARLAV AARDFICAHF TLVARDADFL GLSADELIAI
ISSDGLNVEK EEAVFEAVMR WAGSGDAEAQ AERQRALPTV FESVRCRLLP RAFLESRVER
HPLVRAQPEL LRKVQMVKDA HEGRITTLRK KKKGKDGAGA KEADKGTSKA KAEEDEEAER
ILPGILNDTL RFGMFLQDLI FMISEEGAVA YDPAANECYC ASLSNQVPKN HVSLVTKENQ
VFVAGGLFYN EDNKEDPMSA YFLQFDHLDS EWLGMPPLPS PRCLFGLGEA LNSIYVVGGR
EIKDGERCLD SVMCYDRLSF KWGESDPLPY VVYGHTVLSH MDLVYVIGGK GSDRKCLNKM
CVYDPKKFEW KELAPMQTAR SLFGATVHDG RIIVAAGVTD TGLTSSAEVY SITDNKWAPF
EAFPQERSSL SLVSLVGTLY AIGGFATLET ESGELVPTEL NDIWRYNEEE KKWEGVLREI
AYAAGATFLP VRLNVLCLTK M
