ID   KLHL3_DANRE             Reviewed;         601 AA.
AC   E7F6F9;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   08-MAR-2011, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=Kelch-like protein 3;
GN   Name=klhl3;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
CC   -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC       ubiquitin ligase complex that acts as a regulator of ion transport in
CC       the distal nephron. {ECO:0000250|UniProtKB:Q9UH77}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000250|UniProtKB:Q9UH77}.
CC   -!- SUBUNIT: Component of the BCR(KLHL3) E3 ubiquitin ligase complex, at
CC       least composed of cul3 and klhl3 and rbx1.
CC       {ECO:0000250|UniProtKB:Q9UH77}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q9UH77}. Cytoplasm, cytosol
CC       {ECO:0000250|UniProtKB:Q9UH77}.
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DR   EMBL; BX005228; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; E7F6F9; -.
DR   SMR; E7F6F9; -.
DR   STRING; 7955.ENSDARP00000073620; -.
DR   PaxDb; E7F6F9; -.
DR   ZFIN; ZDB-GENE-120203-4; klhl3.
DR   eggNOG; KOG4441; Eukaryota.
DR   HOGENOM; CLU_004253_14_2_1; -.
DR   InParanoid; E7F6F9; -.
DR   PhylomeDB; E7F6F9; -.
DR   TreeFam; TF329218; -.
DR   UniPathway; UPA00143; -.
DR   PRO; PR:E7F6F9; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0072156; P:distal tubule morphogenesis; ISS:UniProtKB.
DR   GO; GO:0050801; P:ion homeostasis; ISS:UniProtKB.
DR   GO; GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
DR   GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0070294; P:renal sodium ion absorption; ISS:UniProtKB.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR   Gene3D; 2.120.10.80; -; 1.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR011705; BACK.
DR   InterPro; IPR017096; BTB-kelch_protein.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR011043; Gal_Oxase/kelch_b-propeller.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR030578; KLHL3.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   PANTHER; PTHR24412:SF179; PTHR24412:SF179; 1.
DR   Pfam; PF07707; BACK; 1.
DR   Pfam; PF00651; BTB; 1.
DR   Pfam; PF01344; Kelch_1; 6.
DR   PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR   SMART; SM00875; BACK; 1.
DR   SMART; SM00225; BTB; 1.
DR   SMART; SM00612; Kelch; 6.
DR   SUPFAM; SSF50965; SSF50965; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
PE   3: Inferred from homology;
KW   Actin-binding; Cytoplasm; Cytoskeleton; Kelch repeat; Reference proteome;
KW   Repeat; Ubl conjugation pathway.
FT   CHAIN           1..601
FT                   /note="Kelch-like protein 3"
FT                   /id="PRO_0000417533"
FT   DOMAIN          63..130
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT   DOMAIN          165..268
FT                   /note="BACK"
FT   REPEAT          316..361
FT                   /note="Kelch 1"
FT   REPEAT          362..408
FT                   /note="Kelch 2"
FT   REPEAT          410..455
FT                   /note="Kelch 3"
FT   REPEAT          456..504
FT                   /note="Kelch 4"
FT   REPEAT          505..551
FT                   /note="Kelch 5"
FT   REPEAT          553..599
FT                   /note="Kelch 6"
SQ   SEQUENCE   601 AA;  66868 MW;  5C8A62170CA8A3F5 CRC64;
     MVLWGFFLRF RFFLTCGRNC IQKTLDSQDD AKDPGLHTFS HTHMRKAFLL MNDLRSHSRK
     MLCDVLLVAG EVEIPAHRVV LASCSPYFCA MFTGDMSESK ANHVEIRDVD GQTLLKLVDY
     IYSAEIEVSE ENVQVLLPAA SLLQLMDVRQ VCCDFLQTQL HPTNCLGIRA FADLHACTVL
     LSQAHAYAAE QHFTDVMVGE EFMALSLQQV CSLISSDKLT VSTEEKVFEA MVAWIKHDKE
     ARLEHMPKLM EHVRLPLLSR DYLVQIVEEE PLIKNNNTCK DFLIEAMKYH LLPADQRHLI
     KTDRTRPRTP ISLPKVMMVV GGQAPKAIRS VECYDFQEDR WYQVADLPSR RCRAGVVYMA
     GKVYAVGGFN GSLRVRTVDV YDGLKDQWSS IPSMQERRST LGAAVLGDLL YAVGGFDGST
     GLSSVEAYNP KANEWMFVAP MNTRRSSVGV GVVDGKLYAV GGYDGASRQC LSTVEEFNPV
     SNKWCYVSDM STRRSGAGVG VLSGQLYAAG GHDGPLVRKS VEVYDPTTNT WRQVCDMNMC
     RRNAGVCAIN GLLYVIGGDD GSCNLSSVEY YDPAADKWSL IPTNMSNGRS YAGVSVIDKP
     L