KLHL8_MOUSE
ID KLHL8_MOUSE Reviewed; 629 AA.
AC P59280; Q8CD84;
DT 12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Kelch-like protein 8;
GN Name=Klhl8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Hippocampus, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC ubiquitin ligase complex required for The BCR(KLHL8) ubiquitin ligase
CC complex mediates ubiquitination and degradation of RAPSN.
CC {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Component of the BCR(KLHL8) E3 ubiquitin ligase complex, at
CC least composed of CUL3, KLHL8 and RBX1. Interacts with RAPSN (By
CC similarity). {ECO:0000250}.
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DR EMBL; AK031028; BAC27220.1; -; mRNA.
DR EMBL; AK049960; BAC34008.1; -; mRNA.
DR EMBL; AL731554; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC086802; AAH86802.1; -; mRNA.
DR CCDS; CCDS19479.1; -.
DR RefSeq; NP_848856.1; NM_178741.3.
DR RefSeq; XP_006535005.1; XM_006534942.3.
DR RefSeq; XP_017176372.1; XM_017320883.1.
DR AlphaFoldDB; P59280; -.
DR SMR; P59280; -.
DR IntAct; P59280; 1.
DR MINT; P59280; -.
DR STRING; 10090.ENSMUSP00000031254; -.
DR iPTMnet; P59280; -.
DR PhosphoSitePlus; P59280; -.
DR MaxQB; P59280; -.
DR PaxDb; P59280; -.
DR PRIDE; P59280; -.
DR ProteomicsDB; 264851; -.
DR Antibodypedia; 2897; 101 antibodies from 20 providers.
DR Ensembl; ENSMUST00000031254; ENSMUSP00000031254; ENSMUSG00000029312.
DR GeneID; 246293; -.
DR KEGG; mmu:246293; -.
DR UCSC; uc008yjv.1; mouse.
DR CTD; 57563; -.
DR MGI; MGI:2179430; Klhl8.
DR VEuPathDB; HostDB:ENSMUSG00000029312; -.
DR eggNOG; KOG4441; Eukaryota.
DR GeneTree; ENSGT00940000157583; -.
DR InParanoid; P59280; -.
DR OMA; ADQFACE; -.
DR OrthoDB; 709680at2759; -.
DR PhylomeDB; P59280; -.
DR TreeFam; TF329218; -.
DR UniPathway; UPA00143; -.
DR BioGRID-ORCS; 246293; 2 hits in 74 CRISPR screens.
DR PRO; PR:P59280; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; P59280; protein.
DR Bgee; ENSMUSG00000029312; Expressed in primary oocyte and 206 other tissues.
DR ExpressionAtlas; P59280; baseline and differential.
DR Genevisible; P59280; MM.
DR GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:UniProtKB.
DR Gene3D; 2.120.10.80; -; 1.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR011705; BACK.
DR InterPro; IPR017096; BTB-kelch_protein.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR030605; KLHL8.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR24412:SF403; PTHR24412:SF403; 1.
DR Pfam; PF07707; BACK; 1.
DR Pfam; PF00651; BTB; 1.
DR Pfam; PF01344; Kelch_1; 6.
DR PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR SMART; SM00875; BACK; 1.
DR SMART; SM00225; BTB; 1.
DR SMART; SM00612; Kelch; 6.
DR SUPFAM; SSF117281; SSF117281; 2.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Kelch repeat; Reference proteome; Repeat;
KW Ubl conjugation pathway.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9P2G9"
FT CHAIN 2..629
FT /note="Kelch-like protein 8"
FT /id="PRO_0000119109"
FT DOMAIN 76..143
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 178..279
FT /note="BACK"
FT REPEAT 328..375
FT /note="Kelch 1"
FT REPEAT 376..422
FT /note="Kelch 2"
FT REPEAT 424..469
FT /note="Kelch 3"
FT REPEAT 471..516
FT /note="Kelch 4"
FT REPEAT 517..563
FT /note="Kelch 5"
FT REPEAT 565..610
FT /note="Kelch 6"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9P2G9"
FT CONFLICT 29
FT /note="Q -> R (in Ref. 1; BAC34008)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 629 AA; 69788 MW; A91AF0966B8385BF CRC64;
MASESTNGKQ ARSHVTKGRR QYQHQHQQQQ QQQQQVRSRS SVSECDGDDS FIFEANEAWK
DFHGSLLGFY ENGELCDVTL KVGSKLISCH KLVLACVIPY FRAMFLSEMS EAKQALIEIR
DFDGDAVEDL VKFVYSSRLT LTVDNVQPLL YAACILQVEL VARACCEYMQ LHFHPSNCLA
VRAFAESHNR IDLMDMADQY ACEHFTEVVE CEDFVSVSPQ HLHKLLSSSD LNIDSEKQVY
SAAIKWLLAN PQHHPKWLDE TLAQVRLPLL PVDFLMGVVA KEQIVKQNLK CRDLLDEARN
YHLHLSSKPV PDFEYTVRTT PRKHTAGVLF CVGGRGGSGD PFRSIECYSI NKNSWFFGPE
MNSRRRHVGV ISVEGKVYAV GGHDGNEHLG SMEMFDPLTN KWMMKASMNT KRRGIALASL
GGPIYAIGGL DDNTCFSDVE RYDIESDQWS TVAPMNTPRG GVGSVALINH VYAVGGNDGV
ASLSSVERYH PHLDKWIEVK EMGQRRAGNG VSELHGCLYV VGGFDDNSPL SSVERYDPRS
NKWDYVAALT TPRGGVGIAT VMGKIFAVGG HNGNAYLNTV EAFDPVLNKW ELVGPVSHCR
AGAGVAVCDC LTSQIRDVGH GSTNVVDCM
