ID   KLHL9_MOUSE             Reviewed;         617 AA.
AC   Q6ZPT1; Q505G5;
DT   16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 2.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=Kelch-like protein 9;
GN   Name=Klhl9; Synonyms=Kiaa1354;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA   Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA   Saga Y., Nagase T., Ohara O., Koga H.;
RT   "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT   The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT   identified by screening of terminal sequences of cDNA clones randomly
RT   sampled from size-fractionated libraries.";
RL   DNA Res. 10:167-180(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3
CC       ubiquitin-protein ligase complex required for mitotic progression and
CC       cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates
CC       the ubiquitination of AURKB and controls the dynamic behavior of AURKB
CC       on mitotic chromosomes and thereby coordinates faithful mitotic
CC       progression and completion of cytokinesis (By similarity).
CC       {ECO:0000250}.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Component of the BCR(KLHL9-KLHL13) E3 ubiquitin ligase
CC       complex, at least composed of CUL3, KLHL9, KLHL13 and RBX1. Interacts
CC       with AURKB (By similarity). {ECO:0000250}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC98148.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AK129338; BAC98148.1; ALT_INIT; mRNA.
DR   EMBL; BC094556; AAH94556.1; -; mRNA.
DR   EMBL; AL928605; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS38800.1; -.
DR   RefSeq; NP_766459.2; NM_172871.2.
DR   AlphaFoldDB; Q6ZPT1; -.
DR   SMR; Q6ZPT1; -.
DR   BioGRID; 232417; 9.
DR   STRING; 10090.ENSMUSP00000092602; -.
DR   iPTMnet; Q6ZPT1; -.
DR   PhosphoSitePlus; Q6ZPT1; -.
DR   EPD; Q6ZPT1; -.
DR   MaxQB; Q6ZPT1; -.
DR   PaxDb; Q6ZPT1; -.
DR   PeptideAtlas; Q6ZPT1; -.
DR   PRIDE; Q6ZPT1; -.
DR   ProteomicsDB; 264777; -.
DR   Antibodypedia; 24868; 156 antibodies from 24 providers.
DR   Ensembl; ENSMUST00000094993; ENSMUSP00000092602; ENSMUSG00000070923.
DR   GeneID; 242521; -.
DR   KEGG; mmu:242521; -.
DR   UCSC; uc008tnk.1; mouse.
DR   CTD; 55958; -.
DR   MGI; MGI:2180122; Klhl9.
DR   VEuPathDB; HostDB:ENSMUSG00000070923; -.
DR   eggNOG; KOG4441; Eukaryota.
DR   GeneTree; ENSGT00940000154359; -.
DR   HOGENOM; CLU_004253_14_3_1; -.
DR   InParanoid; Q6ZPT1; -.
DR   OMA; RAQEWKS; -.
DR   OrthoDB; 250404at2759; -.
DR   PhylomeDB; Q6ZPT1; -.
DR   TreeFam; TF328485; -.
DR   Reactome; R-MMU-8951664; Neddylation.
DR   Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   UniPathway; UPA00143; -.
DR   BioGRID-ORCS; 242521; 6 hits in 72 CRISPR screens.
DR   ChiTaRS; Klhl9; mouse.
DR   PRO; PR:Q6ZPT1; -.
DR   Proteomes; UP000000589; Chromosome 4.
DR   RNAct; Q6ZPT1; protein.
DR   Bgee; ENSMUSG00000070923; Expressed in medial ganglionic eminence and 267 other tissues.
DR   Genevisible; Q6ZPT1; MM.
DR   GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB.
DR   GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR   GO; GO:0097602; F:cullin family protein binding; ISO:MGI.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; ISO:MGI.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR   GO; GO:0032465; P:regulation of cytokinesis; ISS:UniProtKB.
DR   Gene3D; 2.120.10.80; -; 1.
DR   Gene3D; 3.30.710.10; -; 1.
DR   InterPro; IPR011705; BACK.
DR   InterPro; IPR017096; BTB-kelch_protein.
DR   InterPro; IPR000210; BTB/POZ_dom.
DR   InterPro; IPR015915; Kelch-typ_b-propeller.
DR   InterPro; IPR006652; Kelch_1.
DR   InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR   Pfam; PF07707; BACK; 1.
DR   Pfam; PF00651; BTB; 1.
DR   Pfam; PF01344; Kelch_1; 5.
DR   PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1.
DR   SMART; SM00875; BACK; 1.
DR   SMART; SM00225; BTB; 1.
DR   SMART; SM00612; Kelch; 6.
DR   SUPFAM; SSF117281; SSF117281; 1.
DR   SUPFAM; SSF54695; SSF54695; 1.
DR   PROSITE; PS50097; BTB; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Kelch repeat; Mitosis; Reference proteome;
KW   Repeat; Ubl conjugation pathway.
FT   CHAIN           1..617
FT                   /note="Kelch-like protein 9"
FT                   /id="PRO_0000119111"
FT   DOMAIN          50..119
FT                   /note="BTB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT   DOMAIN          154..255
FT                   /note="BACK"
FT   REPEAT          299..347
FT                   /note="Kelch 1"
FT   REPEAT          348..399
FT                   /note="Kelch 2"
FT   REPEAT          400..446
FT                   /note="Kelch 3"
FT   REPEAT          448..493
FT                   /note="Kelch 4"
FT   REPEAT          495..545
FT                   /note="Kelch 5"
FT   REPEAT          546..594
FT                   /note="Kelch 6"
FT   REGION          595..617
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   617 AA;  69400 MW;  E4670F700065ABFE CRC64;
     MKVSLGNGDM GVSAHLQPCK SGTTRFFTSN THSSVVLQGF DQLRIEGLLC DVTLVPGDGE
     EIFPVHRAMM ASASDYFKAM FTGGMKEKDL MCIKLHGVNK VGLKKIIDFI YTAKLSLNMD
     NLQDTLEAAS FLQILPVLDF CKVFLISGVS LDNCVEVGRI ANTYNLIEVD KYVNNFILKN
     FPALLNTGEF LKLPFERLAF VLSSNSLKHC SELELFKAAC RWLRLEDPRM DYAAKLMKNI
     RFPLMTPQDL INYVQTVDFM RTDNTCVNLL LEASNYQMMP YMQPVMQSDR TAIRSDSTHL
     VTLGGVLRQQ LVVSKELRMY DERAQEWKSL APMDAPRYQH GIAVIGNFLY VVGGQSNYDT
     KGKTAVDTVF RFDPRYNKWM QVASLNEKRT FFHLSALKGH LYAVGGRSAA GELATVECYN
     PRMNEWSYVA KMSEPHYGHA GTVYGGLMYI SGGITHDTFQ NELMCFDPDT DKWTQKAPMT
     TVRGLHCMCT VGDKLYVIGG NHFRGTSDYD DVLSCEYYSP TLDQWTPIAA MLRGQSDVGV
     AVFENKIYVV GGYSWNNRCM VEIVQKYDPE KDEWHKVFDL PESLGGIRAC TLTVFPPEEN
     PGSPSRESPL SAPSDHS