ID   KLK2_BLABR              Reviewed;         280 AA.
AC   Q5FBW1;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=Blarinasin-2;
DE            EC=3.4.21.-;
DE   AltName: Full=Kallikrein-2;
DE   Flags: Precursor; Fragment;
GN   Name=KLK2;
OS   Blarina brevicauda (Northern short-tailed shrew).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Eulipotyphla; Soricidae; Soricinae; Blarina.
OX   NCBI_TaxID=9387;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Salivary gland;
RA   Kita M., Nakamura Y., Kido H., Uemura D.;
RT   "Blarina brevicauda kallikrein mRNA.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: A kallikrein-like protease. It preferentially converts human
CC       high-molecular-weight kininogen (HK) to bradykinin. Is not toxic to
CC       mice (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Salivary glands.
CC   -!- SIMILARITY: Belongs to the peptidase S1 family. Kallikrein subfamily.
CC       {ECO:0000255|PROSITE-ProRule:PRU00274}.
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DR   EMBL; AB105056; BAD89852.1; -; mRNA.
DR   AlphaFoldDB; Q5FBW1; -.
DR   SMR; Q5FBW1; -.
DR   MEROPS; S01.452; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd00190; Tryp_SPc; 1.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001314; Peptidase_S1A.
DR   InterPro; IPR001254; Trypsin_dom.
DR   InterPro; IPR018114; TRYPSIN_HIS.
DR   InterPro; IPR033116; TRYPSIN_SER.
DR   Pfam; PF00089; Trypsin; 1.
DR   PRINTS; PR00722; CHYMOTRYPSIN.
DR   SMART; SM00020; Tryp_SPc; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS50240; TRYPSIN_DOM; 1.
DR   PROSITE; PS00134; TRYPSIN_HIS; 1.
DR   PROSITE; PS00135; TRYPSIN_SER; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Glycoprotein; Hydrolase; Protease; Secreted;
KW   Serine protease; Signal; Zymogen.
FT   SIGNAL          <1..16
FT                   /evidence="ECO:0000255"
FT   PROPEP          17..28
FT                   /id="PRO_0000288942"
FT   CHAIN           29..280
FT                   /note="Blarinasin-2"
FT                   /id="PRO_0000288943"
FT   DOMAIN          29..277
FT                   /note="Peptidase S1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   ACT_SITE        71
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        137
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        232
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        99
FT                   /note="O-linked (GalNAc...) serine"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        121
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        35..192
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        56..72
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        169..238
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        203..217
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   DISULFID        228..253
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00274"
FT   NON_TER         1
SQ   SEQUENCE   280 AA;  31104 MW;  06D3B6F5AE354E36 CRC64;
     YLLLLCLPLT LMGTGAVPPG PSIEIHPRIV GGWECDKHSQ PWQALLTFTN GLDGVCGGVL
     VHPQWVLTAA HCIGDNYKIK LGLHDRFSKD DPFQEFQVSA SFPHPSYNMR LLKLLLSDEM
     NDTYYYEIFP GADFSHDLMM MQLEKPVQLN DAVQVLDLPT QEPQVGSKCH ASGWGSMDPY
     SRNFPRTGKL QCVDLTLMSN NECSRSHIFK ITDDMLCAGH IKGRKDTCGG DSGGPLICDG
     VFQGTTSWGS YPCGKPRMPG VYVKIFSHVD WIREIIATHS