KLP19_CAEEL
ID KLP19_CAEEL Reviewed; 1083 AA.
AC O45935;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Kinesin-like protein klp-19;
GN Name=klp-19 {ECO:0000312|WormBase:Y43F4B.6};
GN ORFNames=Y43F4B.6 {ECO:0000312|WormBase:Y43F4B.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=15452142; DOI=10.1083/jcb.200403036;
RA Powers J., Rose D.J., Saunders A., Dunkelbarger S., Strome S., Saxton W.M.;
RT "Loss of KLP-19 polar ejection force causes misorientation and
RT missegregation of holocentric chromosomes.";
RL J. Cell Biol. 166:991-1001(2004).
CC -!- FUNCTION: Required for chromosome movement and orientation on spindle
CC poles in mitosis and meiosis (PubMed:15452142). May play a role in
CC early anterior-posterior chromosome movement in mitotic embryos
CC (PubMed:15452142). {ECO:0000269|PubMed:15452142}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:15452142}. Nucleus {ECO:0000269|PubMed:15452142}.
CC Cytoplasm, cytoskeleton, spindle {ECO:0000269|PubMed:15452142}.
CC Chromosome {ECO:0000269|PubMed:15452142}. Note=Localizes to nuclei of
CC the distal mitotic zone (PubMed:15452142). During mitotic prophase in
CC embryos, localizes to the nucleoplasm (PubMed:15452142). In
CC prometaphase, localizes to the body of the spindle and the periphery of
CC chromosomes (PubMed:15452142). In anaphase, localizes to the spindle
CC interzone (PubMed:15452142). Localizes to early meiotic prophase nuclei
CC and to the nucleoplasm in late prophase (PubMed:15452142). Localizes to
CC prophase chromosomes before fertilization (PubMed:15452142). During
CC metaphase of meiosis I and meiosis II, localizes to the body of the
CC spindle, around the periphery of chromosomes, and between homologous
CC chromosomes (PubMed:15452142). {ECO:0000269|PubMed:15452142}.
CC -!- TISSUE SPECIFICITY: Expressed in the gonad.
CC {ECO:0000269|PubMed:15452142}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos.
CC {ECO:0000269|PubMed:15452142}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in embryos that
CC undergo early mitotic divisions as in wild-type, but which later then
CC display aberrant patterns of nuclei and arrest before morphogenesis
CC (PubMed:15452142). RNAi-mediated knockdown results in chromosome
CC segregation defects in mitotic embryos whereby chromosomes form a
CC disordered metaphase plate and multiple chromosomes exhibit delays in
CC segregation resulting in the formation of bridges during anaphase
CC (PubMed:15452142). The chromosome bridges stretch, break and sometimes
CC form micronuclei (PubMed:15452142). RNAi-mediated knockdown results in
CC kinetochore orientation defects in anaphase spindles in embryos in
CC which kinetochores do not align on the spindle and some kinetochores
CC stretch across the spindle interzone, parallel to the pole-pole axis
CC (PubMed:15452142). RNAi-mediated knockdown does not cause defects in
CC spindle architecture or cytokinesis in embryos (PubMed:15452142).
CC {ECO:0000269|PubMed:15452142}.
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Kinesin family. {ECO:0000255|PROSITE-ProRule:PRU00283}.
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DR EMBL; BX284603; CAA16335.1; -; Genomic_DNA.
DR PIR; T26844; T26844.
DR RefSeq; NP_499742.1; NM_067341.3.
DR SMR; O45935; -.
DR DIP; DIP-24548N; -.
DR IntAct; O45935; 1.
DR STRING; 6239.Y43F4B.6; -.
DR EPD; O45935; -.
DR PaxDb; O45935; -.
DR PeptideAtlas; O45935; -.
DR EnsemblMetazoa; Y43F4B.6.1; Y43F4B.6.1; WBGene00002229.
DR GeneID; 176750; -.
DR KEGG; cel:CELE_Y43F4B.6; -.
DR UCSC; Y43F4B.6.1; c. elegans.
DR CTD; 176750; -.
DR WormBase; Y43F4B.6; CE16631; WBGene00002229; klp-19.
DR eggNOG; KOG0244; Eukaryota.
DR GeneTree; ENSGT00940000169248; -.
DR HOGENOM; CLU_285326_0_0_1; -.
DR InParanoid; O45935; -.
DR OMA; CEPELQK; -.
DR OrthoDB; 248933at2759; -.
DR PhylomeDB; O45935; -.
DR Reactome; R-CEL-6811434; COPI-dependent Golgi-to-ER retrograde traffic.
DR Reactome; R-CEL-983189; Kinesins.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00002229; Expressed in adult organism and 3 other tissues.
DR GO; GO:0005694; C:chromosome; IDA:WormBase.
DR GO; GO:0000794; C:condensed nuclear chromosome; IDA:WormBase.
DR GO; GO:0005871; C:kinesin complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IDA:WormBase.
DR GO; GO:0005819; C:spindle; IDA:WormBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0003777; F:microtubule motor activity; IBA:GO_Central.
DR GO; GO:0010032; P:meiotic chromosome condensation; IMP:UniProtKB.
DR GO; GO:0051257; P:meiotic spindle midzone assembly; IMP:UniProtKB.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR027640; Kinesin-like_fam.
DR InterPro; IPR019821; Kinesin_motor_CS.
DR InterPro; IPR001752; Kinesin_motor_dom.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR24115; PTHR24115; 2.
DR Pfam; PF00225; Kinesin; 1.
DR PRINTS; PR00380; KINESINHEAVY.
DR SMART; SM00129; KISc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell cycle; Cell division; Chromosome; Coiled coil; Cytoplasm;
KW Cytoskeleton; Meiosis; Mitosis; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..1083
FT /note="Kinesin-like protein klp-19"
FT /id="PRO_0000455829"
FT DOMAIN 6..328
FT /note="Kinesin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT COILED 408..435
FT /evidence="ECO:0000255"
FT COILED 487..650
FT /evidence="ECO:0000255"
FT BINDING 85..92
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
SQ SEQUENCE 1083 AA; 123139 MW; 2410A98C7C142D81 CRC64;
MSSDASLRVV VRARPMNGRE TKEGASRCVQ FYENTKQIVI NESATFTFDA VFADTSDQES
VYETTALPLL DRIFAGFNAT VLAYGQTGSG KTYTMGTEDN VGTDEMRRGI IPRLVSALFQ
RIMNTEAPES FAVTVSMFEV YGDNVYDLLR PDKVKLNVHG DEKNCTVVNL TAVPVIDLKG
ALKQLAVGCH YRTKAETAMN AMSSRSHAVF TVFVEKTATA ECDSAFSAKL QLVDLAGSER
LKKTEAEGNR MKEGININGG LLILSQVIAA LATKQKHIPY RNSVITRVLQ DSLGGNSFTV
FLACISPADS NSQETLNTLR YADRAKQIKN KPIVNKNPKA EEIAILQAQL KRLQKENADL
KQGIAPAEVR FNDANNSAEI LSLKEEVVRK TEQLKERAMK QSECIIRMSA LTQKNSRLEE
DKAKLQSMLT DVRNTVLNEE MLDAAEVVRS IQQVVGDTEE STTLADDDND ETALGGQDDT
IYDTERLPEL QAELDDLEKQ IAMKDENRQK ALDEQRAFIE AMQQRESEKT QLVVRISELE
TEMNKLRQEG KKVTTAAKLA EERRQKLKDL ERQHAEDKKV LNDMKKLQET RRRMEETLKK
TEDELKNLKT QRLRLLREQR AEASKFQAFK QKHEREMAQM KSKLQKREND VAIQKRMTDQ
KLTVLQMRLT EANRANKTLR ELNLKRANRK SSPTNASALQ NMIEEELEHE MCAQRSHWLC
EDLRRQRHDL MQNINTVESM KFEGGKRRRI SASADPNVSV VIEGEEEFEV KRQKELTFLR
ASLETLNEEI KDSLRNETIA GNEERANSRW EKVPAEMRPA FEAVYAQAVA HIRKEIELEF
KLARTKSEFT AKIASKASHE EKRKKEDEEM RAKYRELAQC LEDAKSGLHE KIAFLLCLIK
ENRVDENAIQ QFESLKNQFC DVEQKVKKAS RRKTTNFMGG LTPKPELQRN ERARRAVKYY
GNVVNSEDVT MDDSRHQKRK DHSLLAVEMN RTTDDNVKRR VAMSPIKCDD DTRLTEEDED
IENEAMNNAT FVKDSFNSAT IVLDDSQPSP SNSTFVIGAA PTSEADGVPP IKRKSRRTDL
GPL
