KLRA4_MOUSE
ID KLRA4_MOUSE Reviewed; 263 AA.
AC Q60651; O78026; Q9EPA5;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 18-OCT-2001, sequence version 2.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Killer cell lectin-like receptor 4;
DE AltName: Full=Lymphocyte antigen 49d;
DE Short=Ly-49d;
DE AltName: Full=T-cell surface glycoprotein Ly-49D;
GN Name=Klra4; Synonyms=Ly-49d, Ly49-d, Ly49d;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM D2).
RC STRAIN=C57BL/6J; TISSUE=Spleen;
RX PubMed=8027540;
RA Smith H.R.C., Karlhofer F.M., Yokoyama W.M.;
RT "Ly-49 multigene family expressed by IL-2-activated NK cells.";
RL J. Immunol. 153:1068-1079(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING.
RC STRAIN=C57BL/6J;
RX PubMed=8824161; DOI=10.1007/bf02602811;
RA Silver E.T., Elliott J.F., Kane K.P.;
RT "Alternatively spliced Ly-49D and H transcripts are found in IL-2-activated
RT NK cells.";
RL Immunogenetics 44:478-482(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM D1).
RC STRAIN=NOD, and NOR;
RX PubMed=10925254; DOI=10.4049/jimmunol.165.4.1771;
RA Silver E.T., Gong D.-E., Chang C.S., Amrani A., Santamaria P., Kane K.P.;
RT "Ly-49P activates NK-mediated lysis by recognizing H-2Dd.";
RL J. Immunol. 165:1771-1781(2000).
RN [4]
RP FUNCTION, AND INTERACTION WITH TYROBP.
RX PubMed=9647200;
RA Smith K.M., Wu J., Bakker A.B., Phillips J.H., Lanier L.L.;
RT "Ly-49D and Ly-49H associate with mouse DAP12 and form activating
RT receptors.";
RL J. Immunol. 161:7-10(1998).
CC -!- FUNCTION: Receptor on natural killer (NK) cells for class I MHC.
CC {ECO:0000269|PubMed:9647200}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with the adapter
CC protein TYROBP/DAP12; the interaction leads to natural killer cell
CC activation (PubMed:9647200). {ECO:0000269|PubMed:9647200}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=D1;
CC IsoId=Q60651-1; Sequence=Displayed;
CC Name=D2;
CC IsoId=Q60651-2; Sequence=VSP_003068;
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DR EMBL; U10090; AAA50218.1; -; mRNA.
DR EMBL; L78247; AAC32667.1; -; mRNA.
DR EMBL; AF218079; AAF99592.1; -; mRNA.
DR EMBL; AF218078; AAF99591.1; -; mRNA.
DR CCDS; CCDS57457.1; -. [Q60651-1]
DR PIR; I49049; I49049.
DR RefSeq; NP_001239506.1; NM_001252577.1. [Q60651-1]
DR RefSeq; NP_034779.1; NM_010649.3. [Q60651-2]
DR RefSeq; XP_006505709.1; XM_006505646.1. [Q60651-1]
DR AlphaFoldDB; Q60651; -.
DR SMR; Q60651; -.
DR BioGRID; 200996; 1.
DR STRING; 10090.ENSMUSP00000114004; -.
DR GlyGen; Q60651; 4 sites.
DR MaxQB; Q60651; -.
DR PaxDb; Q60651; -.
DR PRIDE; Q60651; -.
DR ProteomicsDB; 263557; -. [Q60651-1]
DR ProteomicsDB; 263558; -. [Q60651-2]
DR DNASU; 16635; -.
DR Ensembl; ENSMUST00000119096; ENSMUSP00000114004; ENSMUSG00000079852. [Q60651-1]
DR GeneID; 16635; -.
DR KEGG; mmu:16635; -.
DR UCSC; uc009ehh.2; mouse. [Q60651-2]
DR UCSC; uc009ehi.1; mouse. [Q60651-1]
DR CTD; 16635; -.
DR MGI; MGI:101904; Klra4.
DR VEuPathDB; HostDB:ENSMUSG00000079852; -.
DR eggNOG; KOG4297; Eukaryota.
DR GeneTree; ENSGT00390000008117; -.
DR HOGENOM; CLU_049894_1_0_1; -.
DR InParanoid; Q60651; -.
DR OMA; WTESRIE; -.
DR PhylomeDB; Q60651; -.
DR TreeFam; TF336674; -.
DR BioGRID-ORCS; 16635; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Klra4; mouse.
DR PRO; PR:Q60651; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q60651; protein.
DR Bgee; ENSMUSG00000079852; Expressed in umbilical cord and 113 other tissues.
DR ExpressionAtlas; Q60651; baseline and differential.
DR Genevisible; Q60651; MM.
DR GO; GO:0009986; C:cell surface; IDA:UniProtKB.
DR GO; GO:0009897; C:external side of plasma membrane; IDA:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR CDD; cd03593; CLECT_NK_receptors_like; 1.
DR Gene3D; 3.10.100.10; -; 1.
DR InterPro; IPR001304; C-type_lectin-like.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR InterPro; IPR013600; Ly49_N.
DR InterPro; IPR033992; NKR-like_CTLD.
DR Pfam; PF00059; Lectin_C; 1.
DR Pfam; PF08391; Ly49; 1.
DR SMART; SM00034; CLECT; 1.
DR SUPFAM; SSF56436; SSF56436; 1.
DR PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell adhesion; Cell membrane; Disulfide bond;
KW Glycoprotein; Lectin; Membrane; Receptor; Reference proteome;
KW Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..263
FT /note="Killer cell lectin-like receptor 4"
FT /id="PRO_0000046682"
FT TOPO_DOM 1..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..263
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT DOMAIN 139..258
FT /note="C-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT CARBOHYD 87
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 104
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 222
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 146..151
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 164..252
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 168..254
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 233..246
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT VAR_SEQ 39..41
FT /note="Missing (in isoform D2)"
FT /evidence="ECO:0000303|PubMed:8027540"
FT /id="VSP_003068"
FT VARIANT 5
FT /note="E -> K (in strain: NOD and NOR)"
FT VARIANT 29
FT /note="R -> W (in strain: NOD and NOR)"
FT VARIANT 32
FT /note="E -> Q (in strain: NOD and NOR)"
FT VARIANT 35
FT /note="R -> G (in strain: NOD and NOR)"
FT VARIANT 45
FT /note="L -> F (in strain: NOD and NOR)"
FT VARIANT 60
FT /note="T -> I (in strain: NOD and NOR)"
FT VARIANT 79
FT /note="K -> Q (in strain: NOD and NOR)"
FT VARIANT 132
FT /note="Y -> S (in strain: NOD and NOR)"
FT VARIANT 189
FT /note="L -> F (in strain: NOD and NOR)"
SQ SEQUENCE 263 AA; 30872 MW; D0A940A089A9F42D CRC64;
MTEQEDTFSA VRFHKSSGLQ NEMRLKETRK PEKARLRVCS VPWQLIVIAL GILISLRLVT
VAVLMTNIFQ YGQQKHELKE FLKHHNNCSI MQSDINLKDE LLKNKSIECN LLESLNRDQN
ILCDKTRTVL DYLQHTGRGV KVYWFCYGMK CYYFVMDRKP WSRCKQSCQN SSLTLLKIDD
EDELKFLQLV VPSDSCWIGL SYDNKKKDWA WIDNRPSKLA LNTTKYNIRD GGCMFLSKTR
LDNNYCDQSF ICICGKRLDK FPH
