ARAG_ECO57
ID ARAG_ECO57 Reviewed; 504 AA.
AC P0AAF5; P08531;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Arabinose import ATP-binding protein AraG {ECO:0000255|HAMAP-Rule:MF_01721};
DE EC=7.5.2.12 {ECO:0000255|HAMAP-Rule:MF_01721};
GN Name=araG {ECO:0000255|HAMAP-Rule:MF_01721};
GN OrderedLocusNames=Z2953, ECs2608;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Part of the ABC transporter complex AraFGH involved in
CC arabinose import. Responsible for energy coupling to the transport
CC system. {ECO:0000255|HAMAP-Rule:MF_01721}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + L-arabinose(out) = ADP + H(+) + L-arabinose(in) +
CC phosphate; Xref=Rhea:RHEA:30007, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17535, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.5.2.12;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01721};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (AraG),
CC two transmembrane proteins (AraH) and a solute-binding protein (AraF).
CC {ECO:0000255|HAMAP-Rule:MF_01721}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01721}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01721}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Arabinose
CC importer (TC 3.A.1.2.2) family. {ECO:0000255|HAMAP-Rule:MF_01721}.
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DR EMBL; AE005174; AAG56889.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB36031.1; -; Genomic_DNA.
DR PIR; E85803; E85803.
DR PIR; H90954; H90954.
DR RefSeq; NP_310635.1; NC_002695.1.
DR RefSeq; WP_001187819.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; P0AAF5; -.
DR SMR; P0AAF5; -.
DR STRING; 155864.EDL933_2873; -.
DR EnsemblBacteria; AAG56889; AAG56889; Z2953.
DR EnsemblBacteria; BAB36031; BAB36031; ECs_2608.
DR GeneID; 913724; -.
DR KEGG; ece:Z2953; -.
DR KEGG; ecs:ECs_2608; -.
DR PATRIC; fig|386585.9.peg.2734; -.
DR eggNOG; COG1129; Bacteria.
DR HOGENOM; CLU_000604_92_3_6; -.
DR OMA; NVHLGHE; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015612; F:ABC-type L-arabinose transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0042882; P:L-arabinose transmembrane transport; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR017917; ABC_transptr_Ara_ATP-bd_AraG.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR43790:SF6; PTHR43790:SF6; 1.
DR Pfam; PF00005; ABC_tran; 2.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
DR PROSITE; PS51268; ARAG; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Reference proteome; Repeat; Sugar transport;
KW Translocase; Transport.
FT CHAIN 1..504
FT /note="Arabinose import ATP-binding protein AraG"
FT /id="PRO_0000091937"
FT DOMAIN 8..243
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01721"
FT DOMAIN 256..499
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01721"
FT BINDING 40..47
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01721"
FT CONFLICT 187
FT /note="R -> C (in Ref. 1; AAG56889)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 504 AA; 55018 MW; 23B401BCF34CFA3A CRC64;
MQQSTPYLSF RGIGKTFPGV KALTDISFDC YAGQVHALMG ENGAGKSTLL KILSGNYAPT
TGSVVINGQE MSFSDTTAAL NAGVAIIYQE LHLVPEMTVA ENIYLGQLPH KGGIVNRSLL
NYEAGLQLKH LGMDIDPDTP LKYLSIGQWQ MVEIAKALAR NAKIIAFDEP TSSLSAREID
NLFRVIRELR KEGRVILYVS HRMEEIFALS DAITVFKDGR YVKTFTDMQQ VDHDALVQAM
VGRDIGDIYG WQPRSYGEER LRLDAVKAPG VRTPISLAVR SGEIVGLFGL VGAGRSELMK
GMFGGTQITA GQVYIDQQPI DIRKPSHAIA AGMMLCPEDR KAEGIIPVHS VRDNINISAR
RKHVLGGCVI NNGWEENNAD HHIRSLNIKT PGAEQLIMNL SGGNQQKAIL GRWLSEEMKV
ILLDEPTRGI DVGAKHEIYN VIYALAAQGV AVLFASSDLP EVLGVADRIV VMREGEIAGE
LLHEQADERQ ALSLAMPKVS QAVA
