KLRA8_MOUSE
ID KLRA8_MOUSE Reviewed; 266 AA.
AC Q60682; O78027;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Killer cell lectin-like receptor 8;
DE AltName: Full=Lymphocyte antigen 49h;
DE Short=Ly-49h;
DE AltName: Full=T-cell surface glycoprotein Ly-49H;
GN Name=Klra8; Synonyms=Ly-49h, Ly49-h, Ly49H;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM H1).
RC STRAIN=C57BL/6 X CBA; TISSUE=Lung;
RX PubMed=7964501; DOI=10.1084/jem.180.6.2287;
RA Brennan J., Mager D., Jefferies W., Takei F.;
RT "Expression of different members of the Ly-49 gene family defines distinct
RT natural killer cell subsets and cell adhesion properties.";
RL J. Exp. Med. 180:2287-2295(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING.
RC STRAIN=C57BL/6J;
RX PubMed=8824161; DOI=10.1007/bf02602811;
RA Silver E.T., Elliott J.F., Kane K.P.;
RT "Alternatively spliced Ly-49D and H transcripts are found in IL-2-activated
RT NK cells.";
RL Immunogenetics 44:478-482(1996).
RN [3]
RP FUNCTION, AND INTERACTION WITH TYROBP.
RX PubMed=9647200;
RA Smith K.M., Wu J., Bakker A.B., Phillips J.H., Lanier L.L.;
RT "Ly-49D and Ly-49H associate with mouse DAP12 and form activating
RT receptors.";
RL J. Immunol. 161:7-10(1998).
CC -!- FUNCTION: Receptor on natural killer (NK) cells for class I MHC.
CC {ECO:0000269|PubMed:9647200}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. Interacts with the adapter
CC protein TYROBP/DAP12; the interaction leads to natural killer cell
CC activation (PubMed:9647200). {ECO:0000269|PubMed:9647200}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=H1;
CC IsoId=Q60682-1; Sequence=Displayed;
CC Name=H2;
CC IsoId=Q60682-2; Sequence=VSP_003071;
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DR EMBL; U12889; AAA58704.1; -; mRNA.
DR EMBL; L78253; AAC32668.1; -; mRNA.
DR CCDS; CCDS51927.1; -. [Q60682-1]
DR PIR; I49114; I49114.
DR RefSeq; NP_001095090.1; NM_001101620.1. [Q60682-1]
DR RefSeq; NP_034780.1; NM_010650.3. [Q60682-2]
DR PDB; 4JO8; X-ray; 3.20 A; B=110-266.
DR PDBsum; 4JO8; -.
DR AlphaFoldDB; Q60682; -.
DR SMR; Q60682; -.
DR BioGRID; 201000; 1.
DR STRING; 10090.ENSMUSP00000014476; -.
DR GlyGen; Q60682; 2 sites.
DR PaxDb; Q60682; -.
DR PRIDE; Q60682; -.
DR DNASU; 16639; -.
DR Ensembl; ENSMUST00000014476; ENSMUSP00000014476; ENSMUSG00000089727. [Q60682-1]
DR GeneID; 16639; -.
DR KEGG; mmu:16639; -.
DR UCSC; uc009ehp.3; mouse. [Q60682-1]
DR CTD; 16639; -.
DR MGI; MGI:102968; Klra8.
DR VEuPathDB; HostDB:ENSMUSG00000089727; -.
DR eggNOG; KOG4297; Eukaryota.
DR GeneTree; ENSGT00390000008117; -.
DR HOGENOM; CLU_049894_1_0_1; -.
DR InParanoid; Q60682; -.
DR OMA; QERWDSE; -.
DR OrthoDB; 945977at2759; -.
DR PhylomeDB; Q60682; -.
DR TreeFam; TF336674; -.
DR BioGRID-ORCS; 16639; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Klra8; mouse.
DR PRO; PR:Q60682; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q60682; protein.
DR Bgee; ENSMUSG00000089727; Expressed in granulocyte and 27 other tissues.
DR Genevisible; Q60682; MM.
DR GO; GO:0009986; C:cell surface; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; TAS:MGI.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR GO; GO:0009615; P:response to virus; IDA:MGI.
DR CDD; cd03593; CLECT_NK_receptors_like; 1.
DR Gene3D; 3.10.100.10; -; 1.
DR InterPro; IPR001304; C-type_lectin-like.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR InterPro; IPR013600; Ly49_N.
DR InterPro; IPR033992; NKR-like_CTLD.
DR Pfam; PF00059; Lectin_C; 1.
DR Pfam; PF08391; Ly49; 1.
DR SMART; SM00034; CLECT; 1.
DR SUPFAM; SSF56436; SSF56436; 1.
DR PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell adhesion; Cell membrane;
KW Disulfide bond; Glycoprotein; Lectin; Membrane; Receptor;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..266
FT /note="Killer cell lectin-like receptor 8"
FT /id="PRO_0000046686"
FT TOPO_DOM 1..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..66
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..266
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT DOMAIN 143..261
FT /note="C-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT CARBOHYD 87
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 104
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 149..154
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 167..255
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 171..257
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 236..249
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT VAR_SEQ 39..41
FT /note="Missing (in isoform H2)"
FT /evidence="ECO:0000305"
FT /id="VSP_003071"
FT HELIX 113..131
FT /evidence="ECO:0007829|PDB:4JO8"
SQ SEQUENCE 266 AA; 31393 MW; 3CB5A8EF2EB401E2 CRC64;
MSEQEVTFPT MRFHKSSGLN SQVRLEGTQR SRKAGLRVCS VPWQLIVIAL GILCSLRLVI
VAVFVTKFFQ YSQHKQEINE TLNHRHNCSN MQRDFNLKEE MLTNKSIDCR PSYELLEYIK
REQERWDSET KSVSDSSRDT GRGVKYWFCY GTKCYYFIMN KTTWSGCKAN CQHYSVPIVK
IEDEDELKFL QRHVILESYW IGLSYDKKKK EWAWIHNGQS KLDMKIKKMN FTSRGCVFLS
KARIEDTDCN TPYYCICGKK LDKFPD
