ID   KMS2_SCHPO              Reviewed;         457 AA.
AC   O43087; Q9UU62;
DT   23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Karyogamy meiotic segregation protein 2;
GN   Name=kms2; ORFNames=SPBC947.12;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Shimanuki M., Miki F., Ding D.-Q., Hiraoka Y., Niwa O.;
RL   Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 22-153, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=ATCC 38364 / 968;
RX   PubMed=10759889; DOI=10.1046/j.1365-2443.2000.00317.x;
RA   Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T.,
RA   Hiraoka Y.;
RT   "Large-scale screening of intracellular protein localization in living
RT   fission yeast cells by the use of a GFP-fusion genomic DNA library.";
RL   Genes Cells 5:169-190(2000).
RN   [4]
RP   INTERACTION WITH SAD1, AND SUBCELLULAR LOCATION.
RX   PubMed=14655046; DOI=10.1007/s00438-003-0938-8;
RA   Miki F., Kurabayashi A., Tange Y., Okazaki K., Shimanuki M., Niwa O.;
RT   "Two-hybrid search for proteins that interact with Sad1 and Kms1, two
RT   membrane-bound components of the spindle pole body in fission yeast.";
RL   Mol. Genet. Genomics 270:449-461(2004).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-134, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RX   PubMed=18257517; DOI=10.1021/pr7006335;
RA   Wilson-Grady J.T., Villen J., Gygi S.P.;
RT   "Phosphoproteome analysis of fission yeast.";
RL   J. Proteome Res. 7:1088-1097(2008).
CC   -!- SUBUNIT: Interacts with sad1. {ECO:0000269|PubMed:14655046}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, spindle pole body. Cytoplasm. Note=Associates with the spindle
CC       pole body (SPB).
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DR   EMBL; CU329671; CAA17040.1; -; Genomic_DNA.
DR   EMBL; AB055901; BAB32792.1; -; Genomic_DNA.
DR   EMBL; AB027793; BAA87097.1; -; Genomic_DNA.
DR   PIR; T40770; T40770.
DR   RefSeq; NP_595264.1; NM_001021171.2.
DR   AlphaFoldDB; O43087; -.
DR   SMR; O43087; -.
DR   BioGRID; 277761; 13.
DR   IntAct; O43087; 1.
DR   STRING; 4896.SPBC947.12.1; -.
DR   iPTMnet; O43087; -.
DR   MaxQB; O43087; -.
DR   PaxDb; O43087; -.
DR   PRIDE; O43087; -.
DR   EnsemblFungi; SPBC947.12.1; SPBC947.12.1:pep; SPBC947.12.
DR   GeneID; 2541247; -.
DR   KEGG; spo:SPBC947.12; -.
DR   PomBase; SPBC947.12; kms2.
DR   VEuPathDB; FungiDB:SPBC947.12; -.
DR   HOGENOM; CLU_593331_0_0_1; -.
DR   InParanoid; O43087; -.
DR   OMA; ACKSCYT; -.
DR   PhylomeDB; O43087; -.
DR   PRO; PR:O43087; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005737; C:cytoplasm; HDA:PomBase.
DR   GO; GO:0061496; C:half bridge of mitotic spindle pole body; IDA:PomBase.
DR   GO; GO:0034993; C:meiotic nuclear membrane microtubule tethering complex; IPI:PomBase.
DR   GO; GO:0044732; C:mitotic spindle pole body; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0071957; C:old mitotic spindle pole body; IDA:PomBase.
DR   GO; GO:0106166; F:spindle pole body-nuclear membrane anchor activity; EXP:PomBase.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:1903087; P:mitotic spindle pole body duplication; IMP:PomBase.
DR   GO; GO:0140480; P:mitotic spindle pole body insertion into the nuclear envelope; IMP:PomBase.
DR   InterPro; IPR030268; SYNE4.
DR   PANTHER; PTHR21640; PTHR21640; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Meiosis;
KW   Phosphoprotein; Reference proteome.
FT   CHAIN           1..457
FT                   /note="Karyogamy meiotic segregation protein 2"
FT                   /id="PRO_0000084311"
FT   MOD_RES         134
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
SQ   SEQUENCE   457 AA;  53246 MW;  80161FD2DF731AD7 CRC64;
     MDEYIPFDDI VRQYDPDYTG KVSIQAFLEI VDDVDALRLN PEAPLLDNEQ RQSAQDFIKD
     NSEIVVSTSE IKNLFYELTG LDPDTLPVNK LALRENGVLP RKSVAKPQKI SENRIKRKDM
     FYQDASYITP RKGSPLSHST PLSMFRTKNE YGSNKGFSHI NKENADDSLI QQLYERIELQ
     AAELRSKDEQ VKELNARNAK LLEELDSSEE ACKSCYTQAK TWEKKFREAL RDSKEYAAQL
     QTIHEEYEQQ QAHIVRMEEL IHAVEKERKT ETDYMKKESL SEQKERGAFM ESNMILEEKV
     AHLQLENEQL RLFFKEKAPQ PFQNHPPYQN LKITFPSPFF HIPYIPKTET LNDSQFAAGL
     SLLASELESQ KNLLKKFENL KKKSSKDFLS PSTILSNAFS KVSLPNSLIL RVLFFSLLFI
     IGIHIFYFLF FHVATIQQWP FLFWLPSTKF DNRWSPT