KN12E_ARATH
ID KN12E_ARATH Reviewed; 1263 AA.
AC F4J1U4; Q9LXV6;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Kinesin-like protein KIN-12E {ECO:0000305};
GN Name=KIN12E {ECO:0000305};
GN OrderedLocusNames=At3g44050 {ECO:0000312|Araport:AT3G44050};
GN ORFNames=F26G5 {ECO:0000312|EMBL:AL353814},
GN T15B3_190 {ECO:0000312|EMBL:CAB88133.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=11472632; DOI=10.1186/1471-2164-2-2;
RA Reddy A.S., Day I.S.;
RT "Kinesins in the Arabidopsis genome: a comparative analysis among
RT eukaryotes.";
RL BMC Genomics 2:2-2(2001).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16448571; DOI=10.1186/1471-2164-7-18;
RA Richardson D.N., Simmons M.P., Reddy A.S.;
RT "Comprehensive comparative analysis of kinesins in photosynthetic
RT eukaryotes.";
RL BMC Genomics 7:18-18(2006).
RN [5]
RP REVIEW.
RX PubMed=22038119; DOI=10.1007/s00709-011-0343-9;
RA Zhu C., Dixit R.;
RT "Functions of the Arabidopsis kinesin superfamily of microtubule-based
RT motor proteins.";
RL Protoplasma 249:887-899(2012).
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Kinesin family. KIN-12 subfamily.
CC {ECO:0000303|PubMed:16448571}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB88133.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL163975; CAB88133.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL353814; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002686; AEE77856.1; -; Genomic_DNA.
DR PIR; T48959; T48959.
DR RefSeq; NP_189991.2; NM_114273.4.
DR AlphaFoldDB; F4J1U4; -.
DR SMR; F4J1U4; -.
DR STRING; 3702.AT3G44050.1; -.
DR iPTMnet; F4J1U4; -.
DR PaxDb; F4J1U4; -.
DR PRIDE; F4J1U4; -.
DR ProteomicsDB; 238400; -.
DR EnsemblPlants; AT3G44050.1; AT3G44050.1; AT3G44050.
DR GeneID; 823523; -.
DR Gramene; AT3G44050.1; AT3G44050.1; AT3G44050.
DR KEGG; ath:AT3G44050; -.
DR Araport; AT3G44050; -.
DR TAIR; locus:2097184; AT3G44050.
DR eggNOG; KOG4280; Eukaryota.
DR HOGENOM; CLU_005600_1_0_1; -.
DR InParanoid; F4J1U4; -.
DR OMA; AHETAII; -.
DR OrthoDB; 38542at2759; -.
DR PRO; PR:F4J1U4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4J1U4; baseline and differential.
DR GO; GO:0005871; C:kinesin complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0005641; C:nuclear envelope lumen; IDA:TAIR.
DR GO; GO:0009524; C:phragmoplast; IDA:TAIR.
DR GO; GO:0005819; C:spindle; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0003777; F:microtubule motor activity; IBA:GO_Central.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR GO; GO:1901673; P:regulation of mitotic spindle assembly; IEA:InterPro.
DR GO; GO:0051225; P:spindle assembly; IMP:TAIR.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR044986; KIF15/KIN-12E.
DR InterPro; IPR019821; Kinesin_motor_CS.
DR InterPro; IPR001752; Kinesin_motor_dom.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR37739; PTHR37739; 3.
DR Pfam; PF00225; Kinesin; 1.
DR PRINTS; PR00380; KINESINHEAVY.
DR SMART; SM00129; KISc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Coiled coil; Microtubule; Motor protein; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..1263
FT /note="Kinesin-like protein KIN-12E"
FT /id="PRO_0000437192"
FT DOMAIN 93..430
FT /note="Kinesin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT REGION 21..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 679..737
FT /evidence="ECO:0000255"
FT COILED 764..805
FT /evidence="ECO:0000255"
FT COILED 831..881
FT /evidence="ECO:0000255"
FT COILED 905..966
FT /evidence="ECO:0000255"
FT COILED 1091..1168
FT /evidence="ECO:0000255"
FT COILED 1193..1251
FT /evidence="ECO:0000255"
FT COMPBIAS 30..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 174..181
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
SQ SEQUENCE 1263 AA; 142668 MW; FC87CC9942D867BE CRC64;
MPFISETASA IKRRFGFNDR PAPSESLRSV PCTPEANTVS RDNHTHQSLL FSSAVRSMPE
LDEDGAICAG SAQISRSQSF EFNEDPAFWK DHNVQVIIRT RPLSSSEISV QGNNKCVRQD
NGQAITWIGN PESRFTFDLV ADENVSQEQM FKVAGVPMVE NVVAGYNSCM FAYGQTGSGK
THTMLGDIEG GTRRHSVNCG MTPRVFEYLF SRIQKEKEVR KEEKLHFTCR CSFLEIYNEQ
ILDLLDPSSY NLQLREDHKK GIHVENLKEI EVSSARDVIQ QLMQGAANRK VAATNMNRAS
SRSHSVFTCI IESKWVSQGV THHRFARLNL VDLAGSERQK SSGAEGERLK EATNINKSLS
TLGLVIMNLV SVSNGKSVHV PYRDSKLTFL LQDSLGGNSK TIIIANISPS SSCSLETLST
LKFAQRAKLI KNNAIVNEDA SGDVIAMRLQ IQQLKKEVTR LRGMGGVDNQ DMDTISMGCP
ASPMSLKWDG FNGSFTPLTT HKRMSKVKDY EVALVGAFRR EREKDVALQA LTAENEASMK
LEKKREDEIR GLKMMLKLRD SAIKSLQGVT SGKIPVEAHL QKEKGDLMKE IEVLRAQVDR
NQEVTKFATE NLRLKEEIRR LKSQGEEGER DILNQQIQAL QAKLLEALDW KLMHESDSSM
VKEDGNISNM FCSNQNQESK KLSSIQDENE FLRMQAIQNR AEMESLQKSL SFSLDEKERL
QKLVDNLSNE LEGKIRSSGM VGDDDQMEVK TMVQAIACVS QREAEAHETA IKLSKENDDL
RQKIKVLIED NNKLIELYEQ VAEENSSRAW GKIETDSSSN NADAQNSAEI ALEVEKSAAE
EQKKMIGNLE NQLTEMHDEN EKLMSLYENA MKEKDELKRL LSSPDQKKPI EANSDTEMEL
CNISSEKSTE DLNSAKLKLE LAQEKLSISA KTIGVFSSLE ENILDIIKLS KESKETEEKV
KEHQSELGSI KTVSDQTNAR KEVAEKKLAA LRCSLSNFAS SAVYFQQREE RARAHVNSFS
GYLNQKNEEL DVIRSHKREI DAAMGKIQQS EAELKSNIVM LKIKVDEENK RHEEEGVLCT
IDNILRTGKA TDLLKSQEEK TKLQSEMKLS REKLASVRKE VDDMTKKSLK LEKEIKTMET
EIEKSSKTRT ESEMELENTI QEKQTIQEME EQGMSEIQNM IIEIHQLVFE SDLRKEEAMI
IREELIAEEL RAKDVHTNMI ERVENALKTL ENQNNSVSGK IEEEVENVLS LVHEASRLLE
VSH
