KN1_MAIZE
ID KN1_MAIZE Reviewed; 359 AA.
AC P24345;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-1992, sequence version 1.
DT 25-MAY-2022, entry version 140.
DE RecName: Full=Homeotic protein knotted-1;
GN Name=KN-1;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1672445; DOI=10.1038/350241a0;
RA Vollbrecht E., Veit B., Sinha N., Hake S.;
RT "The developmental gene Knotted-1 is a member of a maize homeobox gene
RT family.";
RL Nature 350:241-243(1991).
RN [2]
RP DEVELOPMENTAL STAGE.
RC STRAIN=cv. B73;
RX PubMed=1362381; DOI=10.1242/dev.116.1.21;
RA Smith L.G., Green B., Veit B., Hake S.;
RT "A dominant mutation in the maize homeobox gene, Knotted-1, causes its
RT ectopic expression in leaf cells with altered fates.";
RL Development 116:21-30(1992).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=8616227; DOI=10.1007/bf00017799;
RA Meisel L., Lam E.;
RT "The conserved ELK-homeodomain of KNOTTED-1 contains two regions that
RT signal nuclear localization.";
RL Plant Mol. Biol. 30:1-14(1996).
RN [4]
RP FUNCTION, INTERACTION WITH MBP2C, MUTAGENESIS OF 265-LYS--LYS-267, AND
RP SUBCELLULAR LOCATION.
RX PubMed=17965274; DOI=10.1105/tpc.107.044354;
RA Winter N., Kollwig G., Zhang S., Kragler F.;
RT "MPB2C, a microtubule-associated protein, regulates non-cell-autonomy of
RT the homeodomain protein KNOTTED1.";
RL Plant Cell 19:3001-3018(2007).
CC -!- FUNCTION: Binds to RNA (PubMed:17965274). Possible transcription factor
CC that regulates genes involved in development. Mutations in KN-1 alter
CC leaf development. Foci of cells along the lateral vein do not
CC differentiate properly but continue to divide, forming knots. May
CC participate in the switch from indeterminate to determinate cell fates.
CC Probably binds to the DNA sequence 5'-TGAC-3'.
CC {ECO:0000269|PubMed:17965274}.
CC -!- SUBUNIT: Forms homodimers (By similarity). Binds to MBP2C; this
CC interaction reduces RNA binding capacity (PubMed:17965274).
CC {ECO:0000250|UniProtKB:Q38874, ECO:0000269|PubMed:17965274}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|PROSITE-ProRule:PRU00559, ECO:0000269|PubMed:17965274,
CC ECO:0000269|PubMed:8616227}. Cell junction, plasmodesma
CC {ECO:0000269|PubMed:17965274}. Cytoplasm {ECO:0000269|PubMed:17965274}.
CC Note=Dynamic localization via a plasmodesmata-mediated cell-to-cell
CC transport. This shuttle is repressed by MBP2C binding in cytosolic
CC punctae, at microtubules. {ECO:0000269|PubMed:17965274}.
CC -!- TISSUE SPECIFICITY: Expressed in apical meristems of vegetative and
CC floral stems as well as in the underlying ground meristem. Specifically
CC expressed in vascular bundles developing both in the leaf and stem.
CC Very low levels of expression in leaves.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout apical and vegetative
CC meristem during development. Down-regulated as leaves and floral organs
CC are initiated. {ECO:0000269|PubMed:1362381}.
CC -!- SIMILARITY: Belongs to the TALE/KNOX homeobox family.
CC {ECO:0000255|PROSITE-ProRule:PRU00559}.
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DR EMBL; X61308; CAA43605.1; -; mRNA.
DR PIR; S14283; S14283.
DR RefSeq; NP_001266662.2; NM_001279733.2.
DR AlphaFoldDB; P24345; -.
DR SMR; P24345; -.
DR BioGRID; 951608; 2.
DR STRING; 4577.GRMZM2G017087_P02; -.
DR PaxDb; P24345; -.
DR EnsemblPlants; Zm00001eb055920_T001; Zm00001eb055920_P001; Zm00001eb055920.
DR GeneID; 542391; -.
DR Gramene; Zm00001eb055920_T001; Zm00001eb055920_P001; Zm00001eb055920.
DR KEGG; zma:542391; -.
DR MaizeGDB; 65584; -.
DR eggNOG; KOG0773; Eukaryota.
DR OrthoDB; 1171537at2759; -.
DR Proteomes; UP000007305; Chromosome 1.
DR ExpressionAtlas; P24345; baseline and differential.
DR Genevisible; P24345; ZM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0015630; C:microtubule cytoskeleton; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009506; C:plasmodesma; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IDA:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009901; P:anther dehiscence; IEA:EnsemblPlants.
DR GO; GO:0010930; P:negative regulation of auxin mediated signaling pathway; IEA:EnsemblPlants.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:EnsemblPlants.
DR GO; GO:0010497; P:plasmodesmata-mediated intercellular transport; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR005539; ELK_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR005540; KNOX1.
DR InterPro; IPR005541; KNOX2.
DR Pfam; PF03789; ELK; 1.
DR Pfam; PF05920; Homeobox_KN; 1.
DR Pfam; PF03790; KNOX1; 1.
DR Pfam; PF03791; KNOX2; 1.
DR SMART; SM01188; ELK; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM01255; KNOX1; 1.
DR SMART; SM01256; KNOX2; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51213; ELK; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Cell junction; Cytoplasm; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; RNA-binding; Transcription; Transcription regulation.
FT CHAIN 1..359
FT /note="Homeotic protein knotted-1"
FT /id="PRO_0000048956"
FT DOMAIN 242..262
FT /note="ELK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00559"
FT DNA_BIND 263..326
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 213..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..32
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 265..267
FT /note="KKK->AAA: In KN1M6; defective in cell-to-cell
FT movement capacity. Reduced interaction with MPB2C."
FT /evidence="ECO:0000269|PubMed:17965274"
SQ SEQUENCE 359 AA; 39827 MW; 800FFD82082400FB CRC64;
MEEITQHFGV GASSHGHGHG QHHHHHHHHH PWASSLSAVV APLPPQPPSA GLPLTLNTVA
ATGNSGGSGN PVLQLANGGG LLDACVKAKE PSSSSPYAGD VEAIKAKIIS HPHYYSLLTA
YLECNKVGAP PEVSARLTEI AQEVEARQRT ALGGLAAATE PELDQFMEAY HEMLVKFREE
LTRPLQEAME FMRRVESQLN SLSISGRSLR NILSSGSSEE DQEGSGGETE LPEVDAHGVD
QELKHHLLKK YSGYLSSLKQ ELSKKKKKGK LPKEARQQLL SWWDQHYKWP YPSETQKVAL
AESTGLDLKQ INNWFINQRK RHWKPSEEMH HLMMDGYHTT NAFYMDGHFI NDGGLYRLG
