ID   KN5A_ORYSJ              Reviewed;        1056 AA.
AC   Q5W7C6;
DT   11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Kinesin-like protein KIN-5A {ECO:0000305};
GN   Name=KIN5A {ECO:0000305};
GN   OrderedLocusNames=Os05g0117798 {ECO:0000312|EMBL:BAS91988.1},
GN   LOC_Os05g02670 {ECO:0000305};
GN   ORFNames=OsJ_16908 {ECO:0000312|EMBL:EEE62121.1},
GN   P0496H07.17 {ECO:0000312|EMBL:AAV44208.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA   Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   REVIEW.
RX   PubMed=16530461; DOI=10.1016/j.tplants.2006.02.004;
RA   Vanstraelen M., Inze D., Geelen D.;
RT   "Mitosis-specific kinesins in Arabidopsis.";
RL   Trends Plant Sci. 11:167-175(2006).
RN   [8]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19106179; DOI=10.1093/aob/mcn248;
RA   Guo L., Ho C.M., Kong Z., Lee Y.R., Qian Q., Liu B.;
RT   "Evaluating the microtubule cytoskeleton and its interacting proteins in
RT   monocots by mining the rice genome.";
RL   Ann. Bot. 103:387-402(2009).
CC   -!- FUNCTION: Responsible for microtubule translocation. May be important
CC       for the organization of phragmoplast-specific arrays of microtubules
CC       (By similarity). Plays an essential role in stabilizing the mitotic
CC       spindle. Required during mitotic cytokinesis (By similarity).
CC       {ECO:0000250|UniProtKB:F4IIS5, ECO:0000250|UniProtKB:O23826}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:F4IIS5}. Cytoplasm, cytoskeleton, spindle
CC       {ECO:0000250|UniProtKB:F4IIS5}. Note=Microtubule-associated.
CC       {ECO:0000250|UniProtKB:F4IIS5}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC       superfamily. Kinesin family. KIN-5/BimC subfamily.
CC       {ECO:0000303|PubMed:19106179}.
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DR   EMBL; AC078977; AAV44208.1; -; Genomic_DNA.
DR   EMBL; AP008211; BAH92914.1; -; Genomic_DNA.
DR   EMBL; AP014961; BAS91988.1; -; Genomic_DNA.
DR   EMBL; CM000142; EEE62121.1; -; Genomic_DNA.
DR   EMBL; AK068757; BAG91070.1; -; mRNA.
DR   RefSeq; XP_015639197.1; XM_015783711.1.
DR   RefSeq; XP_015639198.1; XM_015783712.1.
DR   AlphaFoldDB; Q5W7C6; -.
DR   SMR; Q5W7C6; -.
DR   STRING; 4530.OS05T0117798-01; -.
DR   iPTMnet; Q5W7C6; -.
DR   PaxDb; Q5W7C6; -.
DR   PRIDE; Q5W7C6; -.
DR   EnsemblPlants; Os05t0117798-01; Os05t0117798-01; Os05g0117798.
DR   GeneID; 9268593; -.
DR   Gramene; Os05t0117798-01; Os05t0117798-01; Os05g0117798.
DR   KEGG; osa:9268593; -.
DR   eggNOG; KOG0243; Eukaryota.
DR   HOGENOM; CLU_001485_33_0_1; -.
DR   InParanoid; Q5W7C6; -.
DR   OMA; TESHYLE; -.
DR   OrthoDB; 179272at2759; -.
DR   Proteomes; UP000000763; Chromosome 5.
DR   Proteomes; UP000007752; Chromosome 5.
DR   Proteomes; UP000059680; Chromosome 5.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR   GO; GO:0005876; C:spindle microtubule; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR   GO; GO:0008574; F:plus-end-directed microtubule motor activity; IBA:GO_Central.
DR   GO; GO:0007018; P:microtubule-based movement; IEA:InterPro.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR   GO; GO:0090307; P:mitotic spindle assembly; IBA:GO_Central.
DR   GO; GO:0051231; P:spindle elongation; IBA:GO_Central.
DR   Gene3D; 3.40.850.10; -; 1.
DR   InterPro; IPR019821; Kinesin_motor_CS.
DR   InterPro; IPR001752; Kinesin_motor_dom.
DR   InterPro; IPR036961; Kinesin_motor_dom_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00225; Kinesin; 1.
DR   PRINTS; PR00380; KINESINHEAVY.
DR   SMART; SM00129; KISc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR   PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Coiled coil; Cytoplasm; Cytoskeleton; Microtubule;
KW   Motor protein; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..1056
FT                   /note="Kinesin-like protein KIN-5A"
FT                   /id="PRO_0000436271"
FT   DOMAIN          49..395
FT                   /note="Kinesin motor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT   REGION          1..44
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          443..525
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        28..42
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         135..142
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
SQ   SEQUENCE   1056 AA;  117809 MW;  35CAF93A890737AB CRC64;
     MDRRIGLTSP SPKSTEKSGR DLRSGGDANG GANTNSNSIP RGDKEKGVNV QVILRCRPMS
     DEETKSNTPV VISCNERRRE VAATQIIANK QIDRTFAFDK VFGPASKQKD LFEQSISPIV
     NEVLEGYNCT IFAYGQTGTG KTYTMEGGGT RKTKNGELPT DAGVIPRAVR QIFDILEAQC
     AEYSMKVTFL ELYNEEITDL LAPEEPKFPI VPEDKTKKPI ALMEDGKGGV FVRGLEEEVV
     YSAGEIYKIL DKGSAKRRTA ETLLNKQSSR SHSIFSITIH IKELTHEGEE MIKIGKLNLV
     DLAGSENISR SGARDGRARE AGEINKSLLT LGRVINALVE HSGHVPYRDS KLTRLLRDSL
     GGKTKTCIIA TISPSVYCLE ETLSTLDYAH RAKNIKNKPE VNQRMMKSAV IKDLYSEIDR
     LKQEVFAARE KNGIYIPRER YLQEEAEKKA MTEKIERLGA DLEARDKQLV ELKELYDAEQ
     LLSAELSEKL GKTQKDLEDT KNVLHDLEEK YNEAESTIKE KEYVIFNLLK SEKSLVDCAY
     NLRAELENAA ADVSGLFSKI ERKDKIEDGN RSLVQRFRSQ LTNQLDTLHK TVSTSVMQQE
     NHLKEMEDDM QSFVSSKDEA AQGLRESIQK LKLLHGSGIT ALDSLAGEID MNSQSTFERL
     NSQVQSHTSS LEQCFGGIAS EADNLLNELQ CSLSKQEERL TQFAKKQREG HLRAVEASRS
     ISKITAGFFS SLDVHASKLT SILEETQSVQ DQQLLDLEKK FEECAANEEK QLLEKVAEML
     ASSHARKKKL VQTAVGNLRE SAVNRTSHLQ NEISTAQDFT SSVREKWGFY MEETEKNYIE
     DTTAVDSGRS CLAEVLVECK AKTTMGAQQW KNAEDSLFSL GKGNVESADS IVRTGTEANQ
     SLRSKLSSAV STTLEEIDIA NKALLSSIDS SLKLDHDACA NIGSIIKPCH EEISELKGGH
     YHRVVEITEN AGKCLEEEYL VDEPSCSTPR RRQIDLPSME SIEQLRTPDY DELLKSFRES
     RASLKQANGD MKHFLEVQEA TPPSITDPRA PLIARN