KN5C_ARATH
ID KN5C_ARATH Reviewed; 1009 AA.
AC P82266;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 08-FEB-2011, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Kinesin-like protein KIN-5C {ECO:0000305};
DE AltName: Full=AtKRP125b {ECO:0000303|PubMed:11472632};
GN Name=KIN5C {ECO:0000305};
GN OrderedLocusNames=At2g36200 {ECO:0000312|Araport:AT2G36200};
GN ORFNames=F2H17.19 {ECO:0000312|EMBL:AAD21445.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 969-1009.
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY.
RX PubMed=11472632; DOI=10.1186/1471-2164-2-2;
RA Reddy A.S., Day I.S.;
RT "Kinesins in the Arabidopsis genome: a comparative analysis among
RT eukaryotes.";
RL BMC Genomics 2:2-2(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16448571; DOI=10.1186/1471-2164-7-18;
RA Richardson D.N., Simmons M.P., Reddy A.S.;
RT "Comprehensive comparative analysis of kinesins in photosynthetic
RT eukaryotes.";
RL BMC Genomics 7:18-18(2006).
RN [6]
RP REVIEW.
RX PubMed=16530461; DOI=10.1016/j.tplants.2006.02.004;
RA Vanstraelen M., Inze D., Geelen D.;
RT "Mitosis-specific kinesins in Arabidopsis.";
RL Trends Plant Sci. 11:167-175(2006).
RN [7]
RP REVIEW.
RX PubMed=22038119; DOI=10.1007/s00709-011-0343-9;
RA Zhu C., Dixit R.;
RT "Functions of the Arabidopsis kinesin superfamily of microtubule-based
RT motor proteins.";
RL Protoplasma 249:887-899(2012).
CC -!- FUNCTION: Responsible for microtubule translocation. May be important
CC for the organization of phragmoplast-specific arrays of microtubules
CC (By similarity). Plays an essential role in stabilizing the mitotic
CC spindle. Required during mitotic cytokinesis (By similarity).
CC {ECO:0000250|UniProtKB:F4IIS5, ECO:0000250|UniProtKB:O23826}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000250|UniProtKB:F4IIS5}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:F4IIS5}. Note=Microtubule-associated.
CC {ECO:0000250|UniProtKB:F4IIS5}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=P82266-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Kinesin family. KIN-5/BimC subfamily.
CC {ECO:0000303|PubMed:16448571}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD21445.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BX819626; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AC006921; AAD21445.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09215.1; -; Genomic_DNA.
DR EMBL; BX819626; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; H84777; H84777.
DR RefSeq; NP_181162.2; NM_129178.3. [P82266-1]
DR AlphaFoldDB; P82266; -.
DR SMR; P82266; -.
DR STRING; 3702.AT2G36200.2; -.
DR iPTMnet; P82266; -.
DR PaxDb; P82266; -.
DR PRIDE; P82266; -.
DR ProteomicsDB; 238401; -. [P82266-1]
DR EnsemblPlants; AT2G36200.1; AT2G36200.1; AT2G36200. [P82266-1]
DR GeneID; 818192; -.
DR Gramene; AT2G36200.1; AT2G36200.1; AT2G36200. [P82266-1]
DR KEGG; ath:AT2G36200; -.
DR Araport; AT2G36200; -.
DR eggNOG; KOG0243; Eukaryota.
DR InParanoid; P82266; -.
DR OMA; HCRMEVL; -.
DR PhylomeDB; P82266; -.
DR PRO; PR:P82266; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P82266; baseline and differential.
DR Genevisible; P82266; AT.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0072686; C:mitotic spindle; IBA:GO_Central.
DR GO; GO:0005876; C:spindle microtubule; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008017; F:microtubule binding; IEA:InterPro.
DR GO; GO:0008574; F:plus-end-directed microtubule motor activity; IBA:GO_Central.
DR GO; GO:0007018; P:microtubule-based movement; IEA:InterPro.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IBA:GO_Central.
DR GO; GO:0090307; P:mitotic spindle assembly; IBA:GO_Central.
DR GO; GO:0051231; P:spindle elongation; IBA:GO_Central.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR019821; Kinesin_motor_CS.
DR InterPro; IPR001752; Kinesin_motor_dom.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00225; Kinesin; 1.
DR PRINTS; PR00380; KINESINHEAVY.
DR SMART; SM00129; KISc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Motor protein; Nucleotide-binding; Reference proteome.
FT CHAIN 1..1009
FT /note="Kinesin-like protein KIN-5C"
FT /id="PRO_0000125376"
FT DOMAIN 12..359
FT /note="Kinesin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT REGION 862..882
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 987..1009
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 406..526
FT /evidence="ECO:0000255"
FT BINDING 98..105
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
SQ SEQUENCE 1009 AA; 113683 MW; B7554EEFF04676F4 CRC64;
MSSRHDKEKG VNVQVLLRCR PFSDDELRSN APQVLTCNDL QREVAVSQNI AGKHIDRVFT
FDKVFGPSAQ QKDLYDQAVV PIVNEVLEGF NCTIFAYGQT GTGKTYTMEG ECRRSKSAPC
GGLPAEAGVI PRAVKQIFDT LEGQQAEYSV KVTFLELYNE EITDLLAPED LSRVAAEEKQ
KKPLPLMEDG KGGVLVRGLE EEIVTSANEI FTLLERGSSK RRTAETFLNK QSSRSHSLFS
ITIHIKEATP EGEELIKCGK LNLVDLAGSE NISRSGARDG RAREAGEINK SLLTLGRVIS
ALVEHLGHVP YRDSKLTRLL RDSLGGRTKT CIIATVSPAV HCLEETLSTL DYAHRAKNIR
NKPEVNQKMM KSTLIKDLYG EIERLKAEVY ASREKNGVYM PKERYYQEES ERKVMAEQIE
QMGGQIENYQ KQLEELQDKY VGQVRECSDL TTKLDITEKN LSQTCKVLAS TNEELKKSQY
AMKEKDFIIS EQKKSENVLV QQACILQSNL EKATKDNSSL HQKIGREDKL SADNRKVVDN
YQVELSEQIS NLFNRVASCL SQQNVHLQGV NKLSQSRLEA HNKAILEMKK KVKASRDLYS
SHLEAVQNVV RLHKANANAC LEEVSALTTS SACSIDEFLA SGDETTSSLF DELQSALSSH
QGEMALFARE LRQRFHTTME QTQEMSEYTS TFFQKLMEES KNAETRAAEA NDSQINSIID
FQKTYEAQSK SDTDKLIADL TNLVSSHIRR QHELVDSRLH NFKDAVSSNK TFLDEHVSAV
NNLTKDAKRK WETFSMQAEN EAREGADFSA AKHCRMELLL QQSVGHAESA FKHCKITHES
LKEMTSKQVT DVSSLVRSAC DSNEQHDAEV DSARTAAEKD VTKNSDDIIQ QIERMSEDEK
ASVSKILENV RSHEKTLESF QQDQCCQARC IEDKAQETFQ QQYMEYEPTG ATPTKNEPEI
PTKATIESLR AMPIETLVEE FRENNSYESF ATKETKPQQL TRSPLSQVN
