KN7K_ARATH
ID KN7K_ARATH Reviewed; 965 AA.
AC F4J8L3; Q8GYK5; Q9C7B9; Q9LHL9;
DT 08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Kinesin-like protein KIN-7K, chloroplastic {ECO:0000305};
DE Flags: Precursor;
GN Name=KIN7K {ECO:0000305};
GN OrderedLocusNames=At3g12020 {ECO:0000312|Araport:AT3G12020};
GN ORFNames=MEC18.15 {ECO:0000312|EMBL:BAB03114.1},
GN T21B14.15 {ECO:0000312|EMBL:AAG51044.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 424-965.
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP GENE FAMILY.
RX PubMed=11472632; DOI=10.1186/1471-2164-2-2;
RA Reddy A.S., Day I.S.;
RT "Kinesins in the Arabidopsis genome: a comparative analysis among
RT eukaryotes.";
RL BMC Genomics 2:2-2(2001).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16448571; DOI=10.1186/1471-2164-7-18;
RA Richardson D.N., Simmons M.P., Reddy A.S.;
RT "Comprehensive comparative analysis of kinesins in photosynthetic
RT eukaryotes.";
RL BMC Genomics 7:18-18(2006).
RN [7]
RP REVIEW.
RX PubMed=22038119; DOI=10.1007/s00709-011-0343-9;
RA Zhu C., Dixit R.;
RT "Functions of the Arabidopsis kinesin superfamily of microtubule-based
RT motor proteins.";
RL Protoplasma 249:887-899(2012).
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=F4J8L3-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Kinesin family. KIN-7 subfamily.
CC {ECO:0000303|PubMed:16448571}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51044.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAB03114.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAC42213.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AP002040; BAB03114.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC069473; AAG51044.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75137.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63541.1; -; Genomic_DNA.
DR EMBL; AK117552; BAC42213.1; ALT_INIT; mRNA.
DR RefSeq; NP_001325623.1; NM_001337970.1. [F4J8L3-1]
DR RefSeq; NP_187809.3; NM_112036.4. [F4J8L3-1]
DR AlphaFoldDB; F4J8L3; -.
DR SMR; F4J8L3; -.
DR STRING; 3702.AT3G12020.2; -.
DR iPTMnet; F4J8L3; -.
DR PRIDE; F4J8L3; -.
DR ProteomicsDB; 250762; -. [F4J8L3-1]
DR EnsemblPlants; AT3G12020.1; AT3G12020.1; AT3G12020. [F4J8L3-1]
DR EnsemblPlants; AT3G12020.4; AT3G12020.4; AT3G12020. [F4J8L3-1]
DR GeneID; 820376; -.
DR Gramene; AT3G12020.1; AT3G12020.1; AT3G12020. [F4J8L3-1]
DR Gramene; AT3G12020.4; AT3G12020.4; AT3G12020. [F4J8L3-1]
DR KEGG; ath:AT3G12020; -.
DR Araport; AT3G12020; -.
DR eggNOG; KOG0242; Eukaryota.
DR HOGENOM; CLU_004957_0_0_1; -.
DR OMA; NEKTTEC; -.
DR PRO; PR:F4J8L3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4J8L3; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005871; C:kinesin complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0003777; F:microtubule motor activity; IBA:GO_Central.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR027640; Kinesin-like_fam.
DR InterPro; IPR019821; Kinesin_motor_CS.
DR InterPro; IPR001752; Kinesin_motor_dom.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR24115; PTHR24115; 1.
DR Pfam; PF00225; Kinesin; 1.
DR PRINTS; PR00380; KINESINHEAVY.
DR SMART; SM00129; KISc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE 2: Evidence at transcript level;
KW Alternative initiation; ATP-binding; Chloroplast; Coiled coil; Microtubule;
KW Motor protein; Nucleotide-binding; Plastid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN ?..965
FT /note="Kinesin-like protein KIN-7K, chloroplastic"
FT /id="PRO_0000436469"
FT DOMAIN 69..388
FT /note="Kinesin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT REGION 1..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 551..633
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 842..888
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 942..965
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 389..483
FT /evidence="ECO:0000255"
FT COILED 628..703
FT /evidence="ECO:0000255"
FT COILED 738..846
FT /evidence="ECO:0000255"
FT COILED 896..931
FT /evidence="ECO:0000255"
FT COMPBIAS 16..69
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 551..565
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 566..600
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 604..633
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 842..864
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 865..888
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 945..965
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 149..156
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
SQ SEQUENCE 965 AA; 108467 MW; 284F4984170B3D96 CRC64;
MASRQGSKSR KAGLKGADST ASSTTSSSKL YQETSIDGHS SPASSSAQSK QQFFSPDPLP
QTAQRSKENV TVTVRFRPLS PREIRQGEEV AWYADGETIV RNEHNPTIAY AYDRVFGPTT
TTRNVYDIAA HHVVNGAMEG INGTIFAYGV TSSGKTHTMH GDQRSPGIIP LAVKDAFSII
QETPNREFLL RISYMEIYNE VVNDLLNPAG HNLRIREDKQ GTFVEGIKEE VVLSPAHALS
LIAAGEEQRH VGSTNFNLLS SRSHTIFTLT IESSPLGDKS KGEAVHLSQL NLVDLAGSES
SKVETSGVRR KEGSYINKSL LTLGTVISKL TDVRASHVPY RDSKLTRILQ SSLSGHDRVS
LICTVTPASS SSEETHNTLK FAHRAKHIEI QAEQNKIIDE KSLIKKYQRE IRQLKEELEQ
LKQEIVPVPQ LKDIGADDIV LLKQKLEDGQ VKLQSRLEEE EEAKAALLSR IQRLTKLILV
STKNPQASRL PHRFNPRRRH SFGEEELAYL PYKRRDMMDD EQLDLYVSVE GNHEIRDNAY
REEKKTRKHG LLNWLKPKKR DHSSSASDQS SVVKSNSTPS TPQGGGSHLH TESRLSEGSP
LMEQLSEPRE DREALEDSSH EMEIPETSNK MSDELDLLRE QKKILSEEAA LQLSSLKRMS
DEAAKSPQNE EINEEIKVLN DDIKAKNDQI ATLERQIMDF VMTSHEALDK SDIMQAVAEL
RDQLNEKSFE LEVKAADNRI IQQTLNEKTC ECEVLQEEVA NLKQQLSEAL ELAQGTKIKE
LKQDAKELSE SKEQLELRNR KLAEESSYAK GLASAAAVEL KALSEEVAKL MNQNERLAAE
LATQKSPIAQ RNKTGTTTNV RNNGRRESLA KRQEHDSPSM ELKRELRMSK ERELSYEAAL
GEKEQREAEL ERILEETKQR EAYLENELAN MWVLVSKLRR SQGADSEISD SISETRQTEQ
TEGSF
