KN8A_ARATH
ID KN8A_ARATH Reviewed; 725 AA.
AC F4ICA0; Q9FZ77;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Kinesin-like protein KIN-8A {ECO:0000305};
GN Name=KIN8A {ECO:0000305};
GN OrderedLocusNames=At1g18550 {ECO:0000312|Araport:AT1G18550};
GN ORFNames=F25I16.11 {ECO:0000312|EMBL:AAF98419.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=11472632; DOI=10.1186/1471-2164-2-2;
RA Reddy A.S., Day I.S.;
RT "Kinesins in the Arabidopsis genome: a comparative analysis among
RT eukaryotes.";
RL BMC Genomics 2:2-2(2001).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16448571; DOI=10.1186/1471-2164-7-18;
RA Richardson D.N., Simmons M.P., Reddy A.S.;
RT "Comprehensive comparative analysis of kinesins in photosynthetic
RT eukaryotes.";
RL BMC Genomics 7:18-18(2006).
RN [5]
RP REVIEW.
RX PubMed=22038119; DOI=10.1007/s00709-011-0343-9;
RA Zhu C., Dixit R.;
RT "Functions of the Arabidopsis kinesin superfamily of microtubule-based
RT motor proteins.";
RL Protoplasma 249:887-899(2012).
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Kinesin family. KIN-8 subfamily.
CC {ECO:0000303|PubMed:16448571}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF98419.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC026238; AAF98419.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29728.1; -; Genomic_DNA.
DR PIR; A86319; A86319.
DR RefSeq; NP_173290.4; NM_101713.5.
DR AlphaFoldDB; F4ICA0; -.
DR SMR; F4ICA0; -.
DR STRING; 3702.AT1G18550.1; -.
DR iPTMnet; F4ICA0; -.
DR PaxDb; F4ICA0; -.
DR PRIDE; F4ICA0; -.
DR ProteomicsDB; 237142; -.
DR EnsemblPlants; AT1G18550.1; AT1G18550.1; AT1G18550.
DR GeneID; 838436; -.
DR Gramene; AT1G18550.1; AT1G18550.1; AT1G18550.
DR KEGG; ath:AT1G18550; -.
DR Araport; AT1G18550; -.
DR TAIR; locus:2027478; AT1G18550.
DR eggNOG; KOG0242; Eukaryota.
DR HOGENOM; CLU_009927_0_0_1; -.
DR InParanoid; F4ICA0; -.
DR OMA; RSCVKIV; -.
DR OrthoDB; 703086at2759; -.
DR PRO; PR:F4ICA0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4ICA0; baseline and differential.
DR GO; GO:0005871; C:kinesin complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0003777; F:microtubule motor activity; IBA:GO_Central.
DR GO; GO:0008574; F:plus-end-directed microtubule motor activity; IBA:GO_Central.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR019821; Kinesin_motor_CS.
DR InterPro; IPR001752; Kinesin_motor_dom.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00225; Kinesin; 1.
DR PRINTS; PR00380; KINESINHEAVY.
DR SMART; SM00129; KISc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Coiled coil; Microtubule; Motor protein; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..725
FT /note="Kinesin-like protein KIN-8A"
FT /id="PRO_0000437028"
FT DOMAIN 151..481
FT /note="Kinesin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 652..672
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 691..725
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 499..541
FT /evidence="ECO:0000255"
FT COILED 583..617
FT /evidence="ECO:0000255"
FT COMPBIAS 652..667
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 243..250
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
SQ SEQUENCE 725 AA; 81769 MW; D46746D6C59E3E04 CRC64;
MPVSTRSKVM KQERNEQENT NLNLPLRNPH QGLKEKMRAL TLLYEQQKRA SFSLRNPNHN
QSPKPEDQRF KTQLLDSSKK GDRFHRLDGK DSTFVEEDTK ENNVFEADRI FGVSSVPVKP
SGVIRKLSMG NGARNVSEAE KLESLNASVS RILVFVRLRP MGKKERENGS RCCVKVLNKR
DVYLTEFTNE NDYLRLKRLR VRHFTFDSSF PETTTQQEVY STTTGDLVEA VLEGRNGSVF
CYGATGAGKT YTMLGTMENP GVMVLAIKDL FAKVRQRSLD GNHVVHLSYL EVYNETVRDL
LSPGRPLILR EDKQGIVAAG LTQYRAYSTD EVMALLQRGN QNRTTEPTRC NETSSRSHAI
LQVIVEYKTR DASMNIISRV GKLSLIDLAG SERALATDQR TLRSLEGANI NRSLLALSSC
INALVEGKKH IPYRNSKLTQ LLKDSLGGSC NTVMIANISP SSQSFGETQN TLHWADRAKE
IRVKECEVNE EVVQVGEEEG ADQAKLLLEL QKENSELRVQ LAKQQQKLLT LQAENIAAAN
NNNNISLTPP SISSLMTPPS ALTAQQKKKP RHSLLSGTCF TPESLKRTKA EEAVKELQLT
VKALKMEMER MKREHGLQMK KQKDELMKDL CSRKSEKTPE RCKETRRIVT RGSLRPKEKE
KELKSPSHRF ASPVAAAKKR SFWDITVANT SPALDRRKTR SHGLVHQEAP SKLLQPGFAR
PHMKH
