KN8B_ARATH
ID KN8B_ARATH Reviewed; 813 AA.
AC Q9SCJ4; Q0WPV9; Q8L841;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Kinesin-like protein KIN-8B {ECO:0000305};
GN Name=KIN8B {ECO:0000305};
GN OrderedLocusNames=At3g49650 {ECO:0000312|Araport:AT3G49650};
GN ORFNames=T9C5.240 {ECO:0000312|EMBL:CAB62469.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=11472632; DOI=10.1186/1471-2164-2-2;
RA Reddy A.S., Day I.S.;
RT "Kinesins in the Arabidopsis genome: a comparative analysis among
RT eukaryotes.";
RL BMC Genomics 2:2-2(2001).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16448571; DOI=10.1186/1471-2164-7-18;
RA Richardson D.N., Simmons M.P., Reddy A.S.;
RT "Comprehensive comparative analysis of kinesins in photosynthetic
RT eukaryotes.";
RL BMC Genomics 7:18-18(2006).
RN [7]
RP REVIEW.
RX PubMed=22038119; DOI=10.1007/s00709-011-0343-9;
RA Zhu C., Dixit R.;
RT "Functions of the Arabidopsis kinesin superfamily of microtubule-based
RT motor proteins.";
RL Protoplasma 249:887-899(2012).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SCJ4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SCJ4-2; Sequence=VSP_058481, VSP_058482;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Kinesin family. KIN-8 subfamily.
CC {ECO:0000303|PubMed:16448571}.
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DR EMBL; AL132964; CAB62469.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78572.1; -; Genomic_DNA.
DR EMBL; AY120758; AAM53316.1; -; mRNA.
DR EMBL; BT008457; AAP37816.1; -; mRNA.
DR EMBL; AK228951; BAF00840.1; -; mRNA.
DR PIR; T46242; T46242.
DR RefSeq; NP_190534.1; NM_114825.3. [Q9SCJ4-1]
DR AlphaFoldDB; Q9SCJ4; -.
DR SMR; Q9SCJ4; -.
DR STRING; 3702.AT3G49650.1; -.
DR iPTMnet; Q9SCJ4; -.
DR PaxDb; Q9SCJ4; -.
DR PRIDE; Q9SCJ4; -.
DR ProteomicsDB; 237099; -. [Q9SCJ4-1]
DR EnsemblPlants; AT3G49650.1; AT3G49650.1; AT3G49650. [Q9SCJ4-1]
DR GeneID; 824127; -.
DR Gramene; AT3G49650.1; AT3G49650.1; AT3G49650. [Q9SCJ4-1]
DR KEGG; ath:AT3G49650; -.
DR Araport; AT3G49650; -.
DR TAIR; locus:2114728; AT3G49650.
DR eggNOG; KOG0242; Eukaryota.
DR HOGENOM; CLU_001485_2_10_1; -.
DR InParanoid; Q9SCJ4; -.
DR OMA; MMSECSP; -.
DR OrthoDB; 703086at2759; -.
DR PhylomeDB; Q9SCJ4; -.
DR PRO; PR:Q9SCJ4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SCJ4; baseline and differential.
DR GO; GO:0005871; C:kinesin complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0003777; F:microtubule motor activity; IBA:GO_Central.
DR GO; GO:0008574; F:plus-end-directed microtubule motor activity; IBA:GO_Central.
DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central.
DR Gene3D; 3.40.850.10; -; 1.
DR InterPro; IPR027640; Kinesin-like_fam.
DR InterPro; IPR019821; Kinesin_motor_CS.
DR InterPro; IPR001752; Kinesin_motor_dom.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR24115; PTHR24115; 1.
DR Pfam; PF00225; Kinesin; 1.
DR PRINTS; PR00380; KINESINHEAVY.
DR SMART; SM00129; KISc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00411; KINESIN_MOTOR_1; 1.
DR PROSITE; PS50067; KINESIN_MOTOR_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Coiled coil; Microtubule; Motor protein;
KW Nucleotide-binding; Reference proteome.
FT CHAIN 1..813
FT /note="Kinesin-like protein KIN-8B"
FT /id="PRO_0000437029"
FT DOMAIN 14..345
FT /note="Kinesin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT REGION 664..694
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 756..813
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 349..391
FT /evidence="ECO:0000255"
FT COMPBIAS 678..694
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 756..794
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 104..111
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00283"
FT VAR_SEQ 372..373
FT /note="SE -> VS (in isoform 2)"
FT /id="VSP_058481"
FT VAR_SEQ 374..813
FT /note="Missing (in isoform 2)"
FT /id="VSP_058482"
FT CONFLICT 467
FT /note="L -> P (in Ref. 4; BAF00840)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 813 AA; 91051 MW; 0B4DFCAC34280481 CRC64;
MPSIRAPAAK KTTTLTVAVK CRPLMEKERG RDIVRVNNSK EVVVLDPDLS KDYLDRIQNR
TKEKKYCFDH AFGPESTNKN VYRSMSSVIS SVVHGLNATV FAYGSTGSGK TYTMVGTRSD
PGLMVLSLNT IFDMIKSDKS SDEFEVTCSY LEVYNEVIYD LLEKSSGHLE LREDPEQGIV
VAGLRSIKVH SADRILELLN LGNSRRKTES TEMNGTSSRS HAVLEIAVKR RQKNQNQVMR
GKLALVDLAG SERAAETNNG GQKLRDGANI NRSLLALANC INALGKQHKK GLAYVPYRNS
KLTRILKDGL SGNSQTVMVA TISPADSQYH HTVNTLKYAD RAKEIKTHIQ KNIGTIDTHM
SDYQRMIDNL QSEVSQLKTQ LAEKESQLSI KPFERGVERE LSWLDGLSHQ ISENVQDRIN
LQKALFELEE TNLRNRTELQ HLDDAIAKQA TEKDVVEALS SRRQVILDNI RDNDEAGVNY
QRDIEENEKH RCELQDMLNE AINNNGNKTY LHILNQYKLL GMGNTELQFE MAMRDQIIYN
QREAQRNLWN LLMGLGVEEK QVFDLAAKQG ITIEDWSSIA LFPGLPYRKQ TPSFIPANIP
FVGHSYSKSS CTFQSYQDPS SKGQQWAPTP TLCREEHHSS YYFMRQEPPA FVNLRKSHDG
WVGGSRPASR IDTGGNHRRV SYPQTVNNSS PRMASGPTFY QTPQREMLLN TTSPYSSPRV
GLINGATTPS VQPFYGSPRA VTVRNGSYNT PLAPAAVSTK GARNQQRVFG TSPLSGTKGV
KNSSYGQNSH TKLYRGGGTK GHSKGNNTQR QHQ
