KNAT2_ARATH
ID KNAT2_ARATH Reviewed; 310 AA.
AC P46640; Q42414; Q9CAL4;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 3.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Homeobox protein knotted-1-like 2;
DE AltName: Full=Protein ATK1;
DE AltName: Full=Protein KNAT2;
GN Name=KNAT2; Synonyms=ATK1; OrderedLocusNames=At1g70510; ORFNames=F24J13.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=7866029; DOI=10.2307/3869913;
RA Lincoln C., Long J., Yamaguchi J., Serikawa K.A., Hake S.;
RT "A knotted1-like homeobox gene in Arabidopsis is expressed in the
RT vegetative meristem and dramatically alters leaf morphology when
RT overexpressed in transgenic plants.";
RL Plant Cell 6:1859-1876(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Columbia; TISSUE=Seedling;
RX PubMed=7647303; DOI=10.1007/bf00021196;
RA Dockx J., Quaedvlieg N., Keultjes G., Kock G., Weisbeek P.J.,
RA Smeekens S.C.M.;
RT "The homeobox gene ATK1 of Arabidopsis thaliana is expressed in the shoot
RT apex of the seedling and in flowers and inflorescence stems of mature
RT plants.";
RL Plant Mol. Biol. 28:723-737(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP INTERACTION WITH BEL1.
RX PubMed=11701881; DOI=10.2307/3871587;
RA Bellaoui M., Pidkowich M.S., Samach A., Kushalappa K., Kohalmi S.E.,
RA Modrusan Z., Crosby W.L., Haughn G.W.;
RT "The Arabidopsis BELL1 and KNOX TALE homeodomain proteins interact through
RT a domain conserved between plants and animals.";
RL Plant Cell 13:2455-2470(2001).
RN [6]
RP INTERACTION WITH OFP12 AND OFP14.
RX PubMed=15781858; DOI=10.1073/pnas.0501181102;
RA Hackbusch J., Richter K., Muller J., Salamini F., Uhrig J.F.;
RT "A central role of Arabidopsis thaliana ovate family proteins in networking
RT and subcellular localization of 3-aa loop extension homeodomain proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:4908-4912(2005).
RN [7]
RP INTERACTION WITH BEL1; BLH1 AND BLH2.
RX PubMed=17873098; DOI=10.1105/tpc.106.048769;
RA Kumar R., Kushalappa K., Godt D., Pidkowich M.S., Pastorelli S.,
RA Hepworth S.R., Haughn G.W.;
RT "The Arabidopsis BEL1-LIKE HOMEODOMAIN proteins SAW1 and SAW2 act
RT redundantly to regulate KNOX expression spatially in leaf margins.";
RL Plant Cell 19:2719-2735(2007).
RN [8]
RP INTERACTION WITH BZIP30.
RX PubMed=27402171; DOI=10.1111/tpj.13264;
RA Lozano-Sotomayor P., Chavez Montes R.A., Silvestre-Vano M.,
RA Herrera-Ubaldo H., Greco R., Pablo-Villa J., Galliani B.M.,
RA Diaz-Ramirez D., Weemen M., Boutilier K., Pereira A., Colombo L.,
RA Madueno F., Marsch-Martinez N., de Folter S.;
RT "Altered expression of the bZIP transcription factor DRINK ME affects
RT growth and reproductive development in Arabidopsis thaliana.";
RL Plant J. 88:437-451(2016).
CC -!- FUNCTION: May play a role in meristem function, and may be involved in
CC maintaining cells in an undifferentiated, meristematic state. Probably
CC binds to the DNA sequence 5'-TGAC-3'.
CC -!- SUBUNIT: May form heterodimeric complex with the TALE/BELL protein
CC BEL1, BLH1 and BLH2. Interacts with OFP12 and OFP14. Interacts with
CC BZIP30 (PubMed:27402171). {ECO:0000269|PubMed:11701881,
CC ECO:0000269|PubMed:15781858, ECO:0000269|PubMed:17873098,
CC ECO:0000269|PubMed:27402171}.
CC -!- INTERACTION:
CC P46640; Q38897: BEL1; NbExp=3; IntAct=EBI-1153809, EBI-1153783;
CC P46640; Q9SJ56: BLH1; NbExp=3; IntAct=EBI-1153809, EBI-1148379;
CC P46640; Q9FXG8: BLH10; NbExp=4; IntAct=EBI-1153809, EBI-1153895;
CC P46640; Q8S897: BLH5; NbExp=3; IntAct=EBI-1153809, EBI-1153992;
CC P46640; O65685: BLH6; NbExp=3; IntAct=EBI-1153809, EBI-1153881;
CC P46640; Q9LZM8: BLH9; NbExp=3; IntAct=EBI-1153809, EBI-530473;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in shoot apices of
CC seedlings, in the receptacle and developing pistil of flowers and in
CC axillary buds of inflorescence stems.
CC -!- SIMILARITY: Belongs to the TALE/KNOX homeobox family.
CC {ECO:0000255|PROSITE-ProRule:PRU00559}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52468.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U14175; AAA67882.1; -; mRNA.
DR EMBL; X81353; CAA57121.1; -; mRNA.
DR EMBL; X81354; CAA57122.1; -; Genomic_DNA.
DR EMBL; AC010796; AAG52468.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35073.1; -; Genomic_DNA.
DR PIR; S57818; S57818.
DR RefSeq; NP_177208.2; NM_105719.5.
DR AlphaFoldDB; P46640; -.
DR SMR; P46640; -.
DR BioGRID; 28608; 15.
DR IntAct; P46640; 17.
DR STRING; 3702.AT1G70510.1; -.
DR iPTMnet; P46640; -.
DR PaxDb; P46640; -.
DR PRIDE; P46640; -.
DR ProteomicsDB; 238223; -.
DR EnsemblPlants; AT1G70510.1; AT1G70510.1; AT1G70510.
DR GeneID; 843388; -.
DR Gramene; AT1G70510.1; AT1G70510.1; AT1G70510.
DR KEGG; ath:AT1G70510; -.
DR Araport; AT1G70510; -.
DR TAIR; locus:2026810; AT1G70510.
DR eggNOG; KOG0773; Eukaryota.
DR HOGENOM; CLU_040111_0_1_1; -.
DR InParanoid; P46640; -.
DR PhylomeDB; P46640; -.
DR PRO; PR:P46640; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P46640; baseline and differential.
DR Genevisible; P46640; AT.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009736; P:cytokinin-activated signaling pathway; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010094; P:specification of carpel identity; IMP:TAIR.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR005539; ELK_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR005540; KNOX1.
DR InterPro; IPR005541; KNOX2.
DR Pfam; PF03789; ELK; 1.
DR Pfam; PF05920; Homeobox_KN; 1.
DR Pfam; PF03790; KNOX1; 1.
DR Pfam; PF03791; KNOX2; 1.
DR SMART; SM01188; ELK; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM01255; KNOX1; 1.
DR SMART; SM01256; KNOX2; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51213; ELK; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..310
FT /note="Homeobox protein knotted-1-like 2"
FT /id="PRO_0000048958"
FT DOMAIN 208..228
FT /note="ELK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00559"
FT DNA_BIND 229..292
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 178..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 265
FT /note="S -> A (in Ref. 2; CAA57121/CAA57122)"
FT /evidence="ECO:0000305"
FT CONFLICT 310
FT /note="E -> EE (in Ref. 2; CAA57121/CAA57122)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 310 AA; 35638 MW; 0A9F9BA86AB3F36D CRC64;
MDRMCGFRST EDYSEKATLM MPSDYQSLIC STTGDNQRLF GSDELATALS SELLPRIRKA
EDNFSLSVIK SKIASHPLYP RLLQTYIDCQ KVGAPMEIAC ILEEIQRENH VYKRDVAPLS
CFGADPELDE FMETYCDILV KYKTDLARPF DEATTFINKI EMQLQNLCTG PASATALSDD
GAVSSDEELR EDDDIAADDS QQRSNDRDLK DQLLRKFGSH ISSLKLEFSK KKKKGKLPRE
ARQALLDWWN VHNKWPYPTE GDKISLAEET GLDQKQINNW FINQRKRHWK PSENMPFDMM
DDSNETFFTE
