KNAT6_ARATH
ID KNAT6_ARATH Reviewed; 327 AA.
AC Q84JS6; Q948K0; Q9LQD4; Q9LR21;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 138.
DE RecName: Full=Homeobox protein knotted-1-like 6;
DE AltName: Full=Protein KNAT6;
GN Name=KNAT6; OrderedLocusNames=At1g23380; ORFNames=F26F24.25, F28C11.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM KNAT6S).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-327 (ISOFORMS KNAT6S AND KNAT6L),
RP INDUCTION, AND TISSUE SPECIFICITY.
RX PubMed=11311158; DOI=10.1242/dev.128.10.1771;
RA Semiarti E., Ueno Y., Tsukaya H., Iwakawa H., Machida C., Machida Y.;
RT "The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of
RT a symmetric lamina, establishment of venation and repression of meristem-
RT related homeobox genes in leaves.";
RL Development 128:1771-1783(2001).
RN [5]
RP INTERACTION WITH BLH9/PNY.
RX PubMed=15019989; DOI=10.1016/j.gene.2003.12.033;
RA Bhatt A.M., Etchells J.P., Canales C., Lagodienko A., Dickinson H.;
RT "VAAMANA -- a BEL1-like homeodomain protein, interacts with KNOX proteins
RT BP and STM and regulates inflorescence stem growth in Arabidopsis.";
RL Gene 328:103-111(2004).
RN [6]
RP INTERACTION WITH OFP2 AND OFP4.
RX PubMed=15781858; DOI=10.1073/pnas.0501181102;
RA Hackbusch J., Richter K., Muller J., Salamini F., Uhrig J.F.;
RT "A central role of Arabidopsis thaliana ovate family proteins in networking
RT and subcellular localization of 3-aa loop extension homeodomain proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:4908-4912(2005).
RN [7]
RP FUNCTION, INDUCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=16798887; DOI=10.1105/tpc.106.041988;
RA Belles-Boix E., Hamant O., Witiak S.M., Morin H., Traas J., Pautot V.;
RT "KNAT6: an Arabidopsis homeobox gene involved in meristem activity and
RT organ separation.";
RL Plant Cell 18:1900-1907(2006).
CC -!- FUNCTION: Plays a role in meristem function. Contributes to the shoot
CC apical meristem (SAM) maintenance and organ separation by controlling
CC boundary establishment in embryo in a CUC1, CUC2 and STM-dependent
CC manner. Involved in maintaining cells in an undifferentiated,
CC meristematic state. Probably binds to the DNA sequence 5'-TGAC-3'.
CC {ECO:0000269|PubMed:16798887}.
CC -!- SUBUNIT: May form heterodimeric complex with the TALE/BELL protein
CC BLH9/PNY. Interacts with OFP2 and OFP4. {ECO:0000269|PubMed:15019989,
CC ECO:0000269|PubMed:15781858}.
CC -!- INTERACTION:
CC Q84JS6; Q9SJ56: BLH1; NbExp=3; IntAct=EBI-530499, EBI-1148379;
CC Q84JS6; Q9FXG8: BLH10; NbExp=4; IntAct=EBI-530499, EBI-1153895;
CC Q84JS6; Q8S897: BLH5; NbExp=3; IntAct=EBI-530499, EBI-1153992;
CC Q84JS6; O65685: BLH6; NbExp=3; IntAct=EBI-530499, EBI-1153881;
CC Q84JS6; Q9SIW1: BLH7; NbExp=3; IntAct=EBI-530499, EBI-1153967;
CC Q84JS6; Q9LZM8: BLH9; NbExp=4; IntAct=EBI-530499, EBI-530473;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=KNAT6S; Synonyms=short;
CC IsoId=Q84JS6-1; Sequence=Displayed;
CC Name=KNAT6L; Synonyms=long;
CC IsoId=Q84JS6-2; Sequence=VSP_029601;
CC -!- TISSUE SPECIFICITY: Expressed predominantly in shoot apices of
CC seedlings, and, to a lower extent, in rosette leaves.
CC {ECO:0000269|PubMed:11311158}.
CC -!- DEVELOPMENTAL STAGE: First detected in torpedo stage embryos at the
CC boundaries between the presumptive SAM and the cotyledons. Later
CC expressed between the cotyledons and the meristem, and between the
CC cotyledons. In seedlings, localized in stipules and at the boundaries
CC between the SAM and the emerging primordia. Expressed at the site of
CC lateral roots. {ECO:0000269|PubMed:16798887}.
CC -!- INDUCTION: Seems to be repressed by AS2 and AS1 but induced by STM,
CC CUC1 and CUC2. {ECO:0000269|PubMed:11311158,
CC ECO:0000269|PubMed:16798887}.
CC -!- SIMILARITY: Belongs to the TALE/KNOX homeobox family.
CC {ECO:0000255|PROSITE-ProRule:PRU00559}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-4 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79598.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF79598.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAF87007.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC005292; AAF87007.1; ALT_INIT; Genomic_DNA.
DR EMBL; AC007945; AAF79598.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30380.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30381.1; -; Genomic_DNA.
DR EMBL; BT002930; AAO22744.1; -; mRNA.
DR EMBL; BT004370; AAO42364.1; -; mRNA.
DR EMBL; AB072361; BAB69678.1; -; mRNA.
DR EMBL; AB072362; BAB69679.1; -; mRNA.
DR RefSeq; NP_173752.3; NM_102187.4. [Q84JS6-2]
DR RefSeq; NP_850951.2; NM_180620.3. [Q84JS6-1]
DR AlphaFoldDB; Q84JS6; -.
DR SMR; Q84JS6; -.
DR BioGRID; 24185; 18.
DR IntAct; Q84JS6; 18.
DR STRING; 3702.AT1G23380.2; -.
DR iPTMnet; Q84JS6; -.
DR PaxDb; Q84JS6; -.
DR PRIDE; Q84JS6; -.
DR ProteomicsDB; 237120; -. [Q84JS6-1]
DR EnsemblPlants; AT1G23380.1; AT1G23380.1; AT1G23380. [Q84JS6-1]
DR EnsemblPlants; AT1G23380.2; AT1G23380.2; AT1G23380. [Q84JS6-2]
DR GeneID; 838946; -.
DR Gramene; AT1G23380.1; AT1G23380.1; AT1G23380. [Q84JS6-1]
DR Gramene; AT1G23380.2; AT1G23380.2; AT1G23380. [Q84JS6-2]
DR KEGG; ath:AT1G23380; -.
DR Araport; AT1G23380; -.
DR TAIR; locus:2028075; AT1G23380.
DR eggNOG; KOG0773; Eukaryota.
DR HOGENOM; CLU_040111_0_1_1; -.
DR InParanoid; Q84JS6; -.
DR OMA; PSANMME; -.
DR OrthoDB; 944824at2759; -.
DR PhylomeDB; Q84JS6; -.
DR PRO; PR:Q84JS6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84JS6; baseline and differential.
DR Genevisible; Q84JS6; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010073; P:meristem maintenance; IGI:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR005539; ELK_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR005540; KNOX1.
DR InterPro; IPR005541; KNOX2.
DR Pfam; PF03789; ELK; 1.
DR Pfam; PF05920; Homeobox_KN; 1.
DR Pfam; PF03790; KNOX1; 1.
DR Pfam; PF03791; KNOX2; 1.
DR SMART; SM01188; ELK; 1.
DR SMART; SM00389; HOX; 1.
DR SMART; SM01255; KNOX1; 1.
DR SMART; SM01256; KNOX2; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51213; ELK; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..327
FT /note="Homeobox protein knotted-1-like 6"
FT /id="PRO_0000311818"
FT DOMAIN 224..244
FT /note="ELK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00559"
FT DNA_BIND 245..308
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT VAR_SEQ 107
FT /note="K -> KKQ (in isoform KNAT6L)"
FT /evidence="ECO:0000303|PubMed:11311158"
FT /id="VSP_029601"
SQ SEQUENCE 327 AA; 36934 MW; 71147EAD25CD2908 CRC64;
MDGMYNFHSA GDYSDKSVLM MSPESLMFPS DYQALLCSSA GENRVSDVFG SDELLSVAVS
ALSSEAASIA PEIRRNDDNV SLTVIKAKIA CHPSYPRLLQ AYIDCQKVGA PPEIACLLEE
IQRESDVYKQ EVVPSSCFGA DPELDEFMET YCDILVKYKS DLARPFDEAT CFLNKIEMQL
RNLCTGVESA RGVSEDGVIS SDEELSGGDH EVAEDGRQRC EDRDLKDRLL RKFGSRISTL
KLEFSKKKKK GKLPREARQA LLDWWNLHYK WPYPTEGDKI ALADATGLDQ KQINNWFINQ
RKRHWKPSEN MPFAMMDDSS GSFFTEE
