KNG_SALSA
ID KNG_SALSA Reviewed; 375 AA.
AC A0A1S3PBB7;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 1.
DT 03-AUG-2022, entry version 16.
DE RecName: Full=Kininogen;
DE Contains:
DE RecName: Full=Bradykinin;
DE Flags: Precursor;
GN Name=LOC106584303;
OS Salmo salar (Atlantic salmon).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC Salmonidae; Salmoninae; Salmo.
OX NCBI_TaxID=8030;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Muscle;
RA Lien S., Koop B.F., Miller J.R., Kent M.P., Sandve S.R., Nome T.,
RA Hvidsten T.R., Grammes F., Grove H., Gjuvsland A., Karloss J., Vage D.I.,
RA Leong J.S., Minkley D., von Schalburg K., Rondeau E., Zimin A., Walenz B.,
RA Di Genova A., Smith D., Grimholt U., de Jong P., Moen T., Maass A.,
RA Vidal R., Iturra P., Jones S.J.M., Jonassen I., Omholt S.W., Davidson W.S.;
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PROTEIN SEQUENCE OF N-TERMINUS, PARTIAL PROTEIN SEQUENCE, FUNCTION, MASS
RP SPECTROMETRY, AND GLYCOSYLATION.
RX PubMed=10583403; DOI=10.1046/j.1432-1327.1999.00950.x;
RA Yloenen A., Rinne A., Herttuainen J., Bogwald J., Jaervinen M.,
RA Kalkkinen N.;
RT "Atlantic salmon (Salmo salar L.) skin contains a novel kininogen and
RT another cysteine proteinase inhibitor.";
RL Eur. J. Biochem. 266:1066-1072(1999).
RN [3]
RP PROTEIN SEQUENCE OF 72-87 AND 218-244, AND GLYCOSYLATION AT ASN-74 AND
RP ASN-235.
RX PubMed=11447131; DOI=10.1093/glycob/11.7.523;
RA Yloenen A., Kalkkinen N., Saarinen J., Boegwald J., Helin J.;
RT "Glycosylation analysis of two cysteine proteinase inhibitors from Atlantic
RT salmon skin: di-O-acetylated sialic acids are the major sialic acid species
RT on N-glycans.";
RL Glycobiology 11:523-531(2001).
RN [4]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=12397376; DOI=10.1007/s00441-002-0627-7;
RA Taehtinen V., Weber E., Guenther D., Yloenen A., Kalkkinen N., Olsen R.,
RA Jaervinen M., Soederstroem K.O., Rinne A., Bjoerklund H., Boegwald J.;
RT "Immunolocalization of cysteine proteinases (cathepsins) and cysteine
RT proteinase inhibitors (salarin and salmon kininogen) in Atlantic salmon,
RT Salmo salar.";
RL Cell Tissue Res. 310:213-222(2002).
CC -!- FUNCTION: Inhibits papain and ficin (cysteine proteinases) but not
CC trypsin (a serine proteinase). {ECO:0000269|PubMed:10583403}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12397376}. Vacuole
CC {ECO:0000269|PubMed:12397376}.
CC -!- TISSUE SPECIFICITY: Expressed in the skin, liver, intestine, spleen,
CC pancreas and kidney. {ECO:0000269|PubMed:12397376}.
CC -!- PTM: N-glycosylated, with sialylated biantennary complex-type glycans.
CC {ECO:0000269|PubMed:11447131}.
CC -!- PTM: O-glycosylated, sialylated oligosaccharides.
CC {ECO:0000269|PubMed:11447131}.
CC -!- PTM: Bradykinin is released from kininogen by kallikrein.
CC {ECO:0000250|UniProtKB:P01042}.
CC -!- PTM: The N-terminus is blocked. {ECO:0000269|PubMed:10583403}.
CC -!- MASS SPECTROMETRY: [Kininogen]: Mass=52000; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:10583403};
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DR EMBL; AGKD04000030; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_014024862.1; XM_014169387.1.
DR STRING; 8030.ENSSSAP00000084884; -.
DR GeneID; 106584303; -.
DR KEGG; sasa:106584303; -.
DR OMA; VAERTMC; -.
DR OrthoDB; 740995at2759; -.
DR Proteomes; UP000087266; Chromosome ssa23.
DR Bgee; ENSSSAG00000068757; Expressed in liver and 7 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005773; C:vacuole; IDA:UniProtKB.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR CDD; cd00042; CY; 2.
DR InterPro; IPR000010; Cystatin_dom.
DR InterPro; IPR046350; Cystatin_sf.
DR InterPro; IPR027358; Kininogen-type_cystatin_dom.
DR Pfam; PF00031; Cystatin; 2.
DR SMART; SM00043; CY; 2.
DR SUPFAM; SSF54403; SSF54403; 2.
DR PROSITE; PS51647; CYSTATIN_KININOGEN; 2.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Cytoplasm; Direct protein sequencing;
KW Disulfide bond; Glycoprotein; Protease inhibitor; Reference proteome;
KW Signal; Thiol protease inhibitor; Vacuole.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:10583403"
FT CHAIN 24..375
FT /note="Kininogen"
FT /id="PRO_5010208301"
FT PEPTIDE 268..276
FT /note="Bradykinin"
FT /evidence="ECO:0000250|UniProtKB:P01042"
FT /id="PRO_0000455009"
FT DOMAIN 35..139
FT /note="Cystatin kininogen-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00979"
FT DOMAIN 156..260
FT /note="Cystatin kininogen-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00979"
FT REGION 283..375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc) asparagine"
FT /evidence="ECO:0000269|PubMed:11447131"
FT CARBOHYD 235
FT /note="N-linked (GlcNAc) asparagine"
FT /evidence="ECO:0000269|PubMed:11447131"
FT DISULFID 91..102
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00979"
FT DISULFID 115..133
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00979"
FT DISULFID 211..223
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00979"
FT DISULFID 234..254
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00979"
SQ SEQUENCE 375 AA; 41560 MW; 8027FD71627713AA CRC64;
MKLGVRLCVL VVFSLQLWGP GQGQELEPEQ VLAFCDDKDV EAAVDLALVK YNQKLPYGNQ
LALYQILESS KAQNDSCTQY FVEFNSRVTD CPAGGDKVWR DCDYLPTGNK VPRPCKATVH
MSETDKKVLA VFCDPVEAPV VAERTTCLGC PREIDVESED LKDPLTYSIT RFNADSDSSH
HFILNSVGFA TRQVVAGFRY RLMFDMRKSN CSKADHKELN DECHPDPDVE LAHCNSTVDV
APWRHETAEA NVECAPGPLD NFDVFRRRPP GWSPLRNFNN FAEVKTTQAS TASAKEESSE
ESQERSPSAV TMANPEPALP SVAPTTAAES PFHCPSKPWK QFVPPTTLRP AQEKSPTPLP
VVEEGLSDLD LLGKK
