KNL2_ARATH
ID KNL2_ARATH Reviewed; 598 AA.
AC F4KCE9; Q94A31; Q9LZ50;
DT 25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Kinetochore-associated protein KNL-2 homolog {ECO:0000305};
DE AltName: Full=Protein KINETOCHORE NULL 2 {ECO:0000303|PubMed:24014547};
GN Name=KNL2 {ECO:0000303|PubMed:24014547};
GN OrderedLocusNames=At5g02520 {ECO:0000312|Araport:AT5G02520};
GN ORFNames=T22P11_110 {ECO:0000312|EMBL:CAB85989.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 363-598.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=24014547; DOI=10.1105/tpc.113.114736;
RA Lermontova I., Kuhlmann M., Friedel S., Rutten T., Heckmann S.,
RA Sandmann M., Demidov D., Schubert V., Schubert I.;
RT "Arabidopsis kinetochore null2 is an upstream component for centromeric
RT histone H3 variant cenH3 deposition at centromeres.";
RL Plant Cell 25:3389-3404(2013).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF ARG-546 AND TRP-555.
RX PubMed=28062749; DOI=10.1105/tpc.16.00720;
RA Sandmann M., Talbert P., Demidov D., Kuhlmann M., Rutten T., Conrad U.,
RA Lermontova I.;
RT "Targeting of Arabidopsis KNL2 to centromeres depends on the conserved
RT CENPC-k motif in its C terminus.";
RL Plant Cell 29:144-155(2017).
CC -!- FUNCTION: Involved in recognition of centromeres and centromeric
CC localization of the centromere-specific histone CENH3. Required for
CC normal progression of mitosis and meiosis. May play a role in the
CC determination of the epigenetic status of centromeres
CC (PubMed:24014547). Binds DNA and RNA in vitro (PubMed:28062749).
CC {ECO:0000269|PubMed:24014547, ECO:0000269|PubMed:28062749}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:24014547}. Nucleus, nuclear body
CC {ECO:0000269|PubMed:24014547}. Nucleus, nucleolus
CC {ECO:0000269|PubMed:24014547}. Chromosome, centromere
CC {ECO:0000269|PubMed:24014547, ECO:0000269|PubMed:28062749}.
CC Note=Localizes at centromeres during all stages of the mitotic cell
CC cycle, except from metaphase to mid-anaphase (PubMed:24014547).
CC Colocalizes with CENH3 at centromeres in interphase nuclei
CC (PubMed:24014547, PubMed:28062749). {ECO:0000269|PubMed:24014547,
CC ECO:0000269|PubMed:28062749}.
CC -!- TISSUE SPECIFICITY: Expressed in shoot apical meristem, leaf primordia,
CC basal parts of emerging leaves, inflorescence meristems, young
CC inflorescences, developing flower buds, developing sepals and pistils,
CC styles and young siliques. {ECO:0000269|PubMed:24014547}.
CC -!- DISRUPTION PHENOTYPE: Reduced growth, abnormal leaf shape, defect in
CC mature pollen grain formation and aborted seeds, due to defects in
CC mitosis and meiosis. {ECO:0000269|PubMed:24014547}.
CC -!- SIMILARITY: Belongs to the KNL2 family.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB85989.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL162971; CAB85989.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90484.1; -; Genomic_DNA.
DR EMBL; AY050424; AAK91440.1; -; mRNA.
DR EMBL; AY059665; AAL31158.1; -; mRNA.
DR PIR; T48273; T48273.
DR RefSeq; NP_568101.4; NM_120330.7.
DR AlphaFoldDB; F4KCE9; -.
DR STRING; 3702.AT5G02520.1; -.
DR PaxDb; F4KCE9; -.
DR EnsemblPlants; AT5G02520.1; AT5G02520.1; AT5G02520.
DR GeneID; 831892; -.
DR Gramene; AT5G02520.1; AT5G02520.1; AT5G02520.
DR KEGG; ath:AT5G02520; -.
DR Araport; AT5G02520; -.
DR TAIR; locus:2181748; AT5G02520.
DR eggNOG; ENOG502S4QI; Eukaryota.
DR HOGENOM; CLU_042973_0_0_1; -.
DR InParanoid; F4KCE9; -.
DR OMA; DECEVIN; -.
DR PRO; PR:F4KCE9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KCE9; baseline and differential.
DR GO; GO:0010369; C:chromocenter; IDA:TAIR.
DR GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0016604; C:nuclear body; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR InterPro; IPR015216; SANTA.
DR Pfam; PF09133; SANTA; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; DNA-binding; Meiosis;
KW Mitosis; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..598
FT /note="Kinetochore-associated protein KNL-2 homolog"
FT /id="PRO_0000443820"
FT DOMAIN 19..111
FT /note="SANTA"
FT REGION 335..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 445..500
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 520..542
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 538..572
FT /note="Required for localization at centromeres"
FT /evidence="ECO:0000269|PubMed:28062749"
FT REGION 572..598
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 335..355
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 382..398
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 525..542
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 546
FT /note="R->A: Unable to localize at centromeres."
FT /evidence="ECO:0000269|PubMed:28062749"
FT MUTAGEN 555
FT /note="W->R: Unable to localize at centromeres."
FT /evidence="ECO:0000269|PubMed:28062749"
SQ SEQUENCE 598 AA; 66055 MW; C7CF86F884404317 CRC64;
MTEPNLDEDG SKSSFQKTVV LRDWWLIKCP KEFEGKQFGV AGFEESVETR AMRVFTSSPI
TKALDVFTLL ASDGIYITLR GFLNKERVLK NGFNPEISRE FIFGFPPCWE RVCNSCFEGD
SFGTDVNTVP STIEKACPPI LSPCKYSNRN LKDNPAESRE KSNVTETDIA EINDKGGSGA
RDIKTARRRS LHLQIKRILE SSKVRKTAND GDHGSEFLNT AKRGDVERDG CEVINNEDSE
WKLDESEVQN LCNDGDNGSE GFIKAKSSDV EKDKSEAIDN DVISPAVGSG IKHTGADNVD
KVTSASATGE SLTSEQQNGL LVTTASPHSL LKDLAKSSKP EKKGISKKSG KILRSDDNVV
DPMNYSGTKV KSAENKRKID ASKLQSPTSN VAEHSKEGLN NAKSNDVEKD VCVAINNEVI
SPVKGFGKRL SGTDVERLTS KNATKESLTS VQRKGRVKVS KAFQDPLSKG KSKKSEKTLQ
SNSNVVEPMN HFRSEAEEAE ENLSWEKIKR KIDFDVEVTP EKKVKQQKTN AASTDSLGQK
RSRSGRVLVS SLEFWRNQIP VYDMDRNLIQ VKDGSETNSA PSKGKGSDSR KRRNLKIK
