ID   KNO67_HEXDO             Reviewed;          74 AA.
AC   M1E1F0;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   05-JUN-2019, sequence version 2.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=U2-sicaritoxin-Sdo1a {ECO:0000305};
DE            Short=U2-SCRTX-Sdo1a {ECO:0000305};
DE   AltName: Full=S67 {ECO:0000312|PDB:4B2U};
DE   AltName: Full=U2-sicaritoxin-Sd1a {ECO:0000303|PubMed:23342149};
DE            Short=U2-SCRTX-Sd1a {ECO:0000303|PubMed:23342149};
DE   Flags: Precursor;
OS   Hexophthalma dolichocephala (Afrotropical spider) (Sicarius
OS   dolichocephalus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Haplogynae; Scytodoidea; Sicariidae; Sicarius.
OX   NCBI_TaxID=571538;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], STRUCTURE BY NMR OF 39-74, AND DISULFIDE BOND.
RC   TISSUE=Venom gland;
RX   PubMed=23342149; DOI=10.1371/journal.pone.0054401;
RA   Loening N.M., Wilson Z.N., Zobel-Thropp P.A., Binford G.J.;
RT   "Solution structures of two homologous venom peptides from Sicarius
RT   dolichocephalus.";
RL   PLoS ONE 8:E54401-E54401(2013).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:23342149}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:23342149}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC       {ECO:0000269|PubMed:23342149}.
CC   -!- CAUTION: For expression reasons, the sequence was engineered with a Gly
CC       residue instead of a Pro at position 39. {ECO:0000305|PubMed:23342149}.
CC   -!- WEB RESOURCE: Name=Biological Magnetic Resonance Data Bank;
CC       URL="http://www.bmrb.wisc.edu/data_library/summary/index.php?bmrbId=18600";
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DR   PDB; 4B2U; NMR; -; A=39-74.
DR   PDBsum; 4B2U; -.
DR   AlphaFoldDB; M1E1F0; -.
DR   SMR; M1E1F0; -.
DR   ArachnoServer; AS002050; U2-sicaritoxin-Sdo1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   3D-structure; Disulfide bond; Knottin; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..39
FT                   /evidence="ECO:0000305|PubMed:23342149"
FT                   /id="PRO_0000447420"
FT   PEPTIDE         40..74
FT                   /note="U2-sicaritoxin-Sdo1a"
FT                   /evidence="ECO:0000305|PubMed:23342149"
FT                   /id="PRO_0000447421"
FT   DISULFID        42..59
FT                   /evidence="ECO:0000269|PubMed:23342149,
FT                   ECO:0007744|PDB:4B2U"
FT   DISULFID        49..62
FT                   /evidence="ECO:0000269|PubMed:23342149,
FT                   ECO:0007744|PDB:4B2U"
FT   DISULFID        58..71
FT                   /evidence="ECO:0000269|PubMed:23342149,
FT                   ECO:0007744|PDB:4B2U"
FT   HELIX           52..54
FT                   /evidence="ECO:0007829|PDB:4B2U"
FT   STRAND          57..65
FT                   /evidence="ECO:0007829|PDB:4B2U"
FT   STRAND          68..72
FT                   /evidence="ECO:0007829|PDB:4B2U"
SQ   SEQUENCE   74 AA;  8806 MW;  B7A2BF19F172EBAD CRC64;
     MKLSFCFFLC AIVLFSFAEA RINPNQLKRL RELVRDDEPT YCIELGERCP NPREGDWCCH
     KCVPEGKRFY CRDQ