KNOB_PLAFG
ID KNOB_PLAFG Reviewed; 634 AA.
AC P09346; P13818;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Knob-associated histidine-rich protein;
DE Short=KAHRP;
DE Short=KP;
DE Flags: Precursor;
OS Plasmodium falciparum (isolate FCR-3 / Gambia).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=5838;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3313387; DOI=10.1073/pnas.84.20.7139;
RA Pologe L.G., Pavlovec A., Shio H., Ravetch J.V.;
RT "Primary structure and subcellular localization of the knob-associated
RT histidine-rich protein of Plasmodium falciparum.";
RL Proc. Natl. Acad. Sci. U.S.A. 84:7139-7143(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2828945; DOI=10.1016/0166-6851(87)90124-1;
RA Sharma Y.D., Kilejian A.;
RT "Structure of the knob protein (KP) gene of Plasmodium falciparum.";
RL Mol. Biochem. Parasitol. 26:11-16(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE OF 1-270.
RX PubMed=3532126; DOI=10.1073/pnas.83.20.7938;
RA Kilejian A., Sharma Y.D., Karoui H., Naslund L.;
RT "Histidine-rich domain of the knob protein of the human malaria parasite
RT Plasmodium falciparum.";
RL Proc. Natl. Acad. Sci. U.S.A. 83:7938-7941(1986).
CC -!- FUNCTION: KAHRP might mimick human histidine-rich glycoproteins to
CC anchor host thrombospondin or a parasite analog in a binding complex
CC with the endothelial cell receptor.
CC -!- SUBCELLULAR LOCATION: Secreted. Note=Cytoplasmic side of the membrane
CC of infected erythrocytes.
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DR EMBL; J02972; AAA29629.1; -; mRNA.
DR EMBL; M19881; AAA29632.1; -; Genomic_DNA.
DR EMBL; M14210; AAA29631.1; -; mRNA.
DR PIR; A26480; A26480.
DR PIR; A28412; A28412.
DR PIR; A54495; A54495.
DR AlphaFoldDB; P09346; -.
DR BMRB; P09346; -.
DR TCDB; 9.B.367.1.1; the plasmodium falciparum virulence factor trafficking system (pfvfts) family.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR040805; EMP3/KAHRP_N.
DR Pfam; PF17986; EKAL; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Malaria; Repeat; Secreted; Signal.
FT SIGNAL 1..34
FT /evidence="ECO:0000255"
FT CHAIN 35..634
FT /note="Knob-associated histidine-rich protein"
FT /id="PRO_0000024545"
FT REPEAT 540..549
FT /note="1"
FT REPEAT 550..559
FT /note="2"
FT REPEAT 560..569
FT /note="3"
FT REPEAT 570..579
FT /note="4"
FT REGION 57..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 347..634
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 540..580
FT /note="4 X 10 AA tandem repeats of [TS]-[KE]-[GE]-A-T-K-
FT [EG]-A-S-T"
FT COMPBIAS 57..89
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..136
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 352..381
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 390..409
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 418..488
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 489..509
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 518..533
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 543..618
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 42
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 260
FT /note="A -> T (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 492
FT /note="V -> A (in Ref. 2; AAA29632)"
FT /evidence="ECO:0000305"
FT CONFLICT 507
FT /note="P -> T (in Ref. 2; AAA29632)"
FT /evidence="ECO:0000305"
FT CONFLICT 539
FT /note="A -> T (in Ref. 2; AAA29632)"
FT /evidence="ECO:0000305"
FT CONFLICT 543
FT /note="A -> T (in Ref. 2; AAA29632)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 634 AA; 69149 MW; 4D5E28EA6171DE7D CRC64;
MKSFKNKNTL RRKKAFPVFT KILLVSFLVW VLKCSNNCNN GNGSGDSFDF RNKRTLAQKQ
HEHHHHHHHQ HQHQHQAPHQ AHHHHHHGEV NHQAPQVHQQ VHGQDQAHHH HHHHHHQLQP
QQLQGTVANP PSNEPVVKTQ VFREARPGGG FKAYEEKYES KHYKLKENVV DGKKDCDEKY
EAANYAFSEE CPYTVNDYSQ ENGPNIFALR KRFPLGMNDE DEEGKEALAI KDKLPGGLDE
YQNQLYGICN ETCTTCGPAA IDYVPADAPN GYAYGGSAHD GSHGNLRGHG NKGSEGYGYE
APYNPGFNGA PGSNGMQNYV PPHGAGYSAP YGVPHGAAHG SRYSSFSSVN KYGKHGDEKH
HSSKKHEGND GEGEKKKKSK KHKDHDGEKK KSKKHKDNED AESVKSKKHK SHDCEKKKSK
KHKDNEDAES VKSKKSVKEK GEKHNGKKPC SKKTNEENKN KEKTNNLKSD GSKAHEKKEN
ETKNTAGENK KVDSTSADNK STNAATPGAK DKTQGGKTDK TGASTNAATN KGQCAAEGAT
KGATKEASTS KEATKEASTS KGATKEASTT EGATKGASTT AGSTTGATTG ANAVQSKDGT
ADKNAANNGE QVMSRGQAQL QEAGKKKKKR GCCG
