KNOS6_ORYSJ
ID KNOS6_ORYSJ Reviewed; 361 AA.
AC P46609; Q0DNY5; Q10DL4; Q10DL5; Q75GJ3;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Homeobox protein knotted-1-like 6;
DE AltName: Full=Homeobox protein OSH1;
DE AltName: Full=Homeobox protein knotted-1-like 1;
DE Short=Oskn1;
GN Name=OSH1; OrderedLocusNames=Os03g0727000, LOC_Os03g51690;
GN ORFNames=OSJNBa0013A09.5;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Nipponbare; TISSUE=Seedling;
RX PubMed=8104574; DOI=10.2307/3869625;
RA Matsuoka M., Ichikawa H., Saito A., Tada Y., Fujimura T., Kano-Murakami Y.;
RT "Expression of a rice homeobox gene causes altered morphology of transgenic
RT plants.";
RL Plant Cell 5:1039-1048(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=8755613; DOI=10.1073/pnas.93.15.8117;
RA Sato Y., Hong S.-K., Tagiri A., Kitano H., Yamamoto N., Nagato Y.,
RA Matsuoka M.;
RT "A rice homeobox gene, OSH1, is expressed before organ differentiation in a
RT specific region during early embryogenesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:8117-8122(1996).
RN [7]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=9869405; DOI=10.1023/a:1006065622251;
RA Sato Y., Sentoku N., Nagato Y., Matsuoka M.;
RT "Isolation and characterization of a rice homeobox gene, OSH15.";
RL Plant Mol. Biol. 38:983-998(1998).
RN [8]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=10488233; DOI=10.2307/3871044;
RA Sentoku N., Sato Y., Kurata N., Ito Y., Kitano H., Matsuoka M.;
RT "Regional expression of the rice KN1-type homeobox gene family during
RT embryo, shoot, and flower development.";
RL Plant Cell 11:1651-1664(1999).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18430022; DOI=10.1111/j.1742-4658.2008.06424.x;
RA Jain M., Tyagi A.K., Khurana J.P.;
RT "Genome-wide identification, classification, evolutionary expansion and
RT expression analyses of homeobox genes in rice.";
RL FEBS J. 275:2845-2861(2008).
RN [10]
RP FUNCTION, INTERACTION WITH FTIP7, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=29915329; DOI=10.1038/s41477-018-0175-0;
RA Song S., Chen Y., Liu L., See Y.H.B., Mao C., Gan Y., Yu H.;
RT "OsFTIP7 determines auxin-mediated anther dehiscence in rice.";
RL Nat. Plants 4:495-504(2018).
CC -!- FUNCTION: Transcription factor that regulates genes involved in
CC development. May be involved in shoot formation during embryogenesis.
CC Overexpression in transgenic plants causes altered leaf morphology
CC (PubMed:10488233, PubMed:8755613, PubMed:9869405). Regulates anther
CC dehiscence via direct repression of the auxin biosynthetic gene YUCCA4
CC (PubMed:29915329). Binds to the DNA sequence 5'-TGAC-3' in the promoter
CC of the YUCCA4 gene and represses its activity during anther development
CC (PubMed:29915329). Reduction of auxin levels at late stage of anther
CC development, after meiosis of microspore mother cells, is necessary for
CC normal anther dehiscence and seed setting (PubMed:29915329).
CC {ECO:0000269|PubMed:10488233, ECO:0000269|PubMed:29915329,
CC ECO:0000269|PubMed:8755613, ECO:0000269|PubMed:9869405}.
CC -!- SUBUNIT: Interacts with FTIP7. {ECO:0000269|PubMed:29915329}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:29915329}. Cytoplasm
CC {ECO:0000269|PubMed:29915329}. Note=Localizes mainly in the nucleus
CC (PubMed:29915329). Translocation from the cytoplasm to the nucleus is
CC facilitated by FTIP7 (PubMed:29915329). {ECO:0000269|PubMed:29915329}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P46609-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P46609-2; Sequence=VSP_036180, VSP_036181;
CC -!- TISSUE SPECIFICITY: Expressed predominantly in shoot apices. Also found
CC to a lesser extent in glumes.
CC -!- DEVELOPMENTAL STAGE: Expressed in the globular stage embryo 2 days
CC after pollination (DAP) in a restricted small region just below the
CC center of the ventral region of the embryo, where the shoot apex arises
CC later. From 3 to 4 DAP expressed in an enlarged ventral region of
CC embryo, corresponding to the expected epiblast and radicle,
CC respectively. At the shoot apex differentiation stage (4-5 DAP),
CC expressed in the shoot apex, epiblast, radicle primordia, and in their
CC intervening tissues. Expression in the radicle is observed in the cells
CC surrounding the root apical meristem in a donut shape but not in the
CC meristem. During the first and second leaf primordium formation,
CC expression pattern is maintained, but decreases. During inflorescence
CC development, expressed only in the corpus of the rachis primordium but
CC not in the tunica layer (L1). After floral induction, expressed in both
CC tunica and corpus but not in floral organ primordia. Later in flower
CC development, expressed in the corpus of the floral meristem.
CC {ECO:0000269|PubMed:10488233, ECO:0000269|PubMed:8755613,
CC ECO:0000269|PubMed:9869405}.
CC -!- DISRUPTION PHENOTYPE: Early termination of shoot meristems, reduced
CC leaf formation and reduced seed setting. {ECO:0000269|PubMed:29915329}.
CC -!- SIMILARITY: Belongs to the TALE/KNOX homeobox family.
CC {ECO:0000255|PROSITE-ProRule:PRU00559}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF13053.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; D16507; BAA03959.1; -; mRNA.
DR EMBL; AC145380; AAS07158.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF98651.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF98653.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF13053.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014959; BAS86183.1; -; Genomic_DNA.
DR RefSeq; XP_015629392.1; XM_015773906.1. [P46609-1]
DR AlphaFoldDB; P46609; -.
DR SMR; P46609; -.
DR STRING; 4530.OS03T0727000-01; -.
DR PaxDb; P46609; -.
DR PRIDE; P46609; -.
DR EnsemblPlants; Os03t0727000-02; Os03t0727000-02; Os03g0727000. [P46609-1]
DR GeneID; 4333973; -.
DR Gramene; Os03t0727000-02; Os03t0727000-02; Os03g0727000. [P46609-1]
DR KEGG; osa:4333973; -.
DR InParanoid; P46609; -.
DR PlantReactome; R-OSA-9631623; Regulation of embryo development.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; P46609; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IMP:Gramene.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0009901; P:anther dehiscence; IMP:UniProtKB.
DR GO; GO:0010930; P:negative regulation of auxin mediated signaling pathway; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:Gramene.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR005539; ELK_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR005540; KNOX1.
DR InterPro; IPR005541; KNOX2.
DR Pfam; PF03789; ELK; 1.
DR Pfam; PF05920; Homeobox_KN; 1.
DR Pfam; PF03790; KNOX1; 1.
DR Pfam; PF03791; KNOX2; 1.
DR SMART; SM01188; ELK; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM01255; KNOX1; 1.
DR SMART; SM01256; KNOX2; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51213; ELK; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..361
FT /note="Homeobox protein knotted-1-like 6"
FT /id="PRO_0000049203"
FT DOMAIN 242..262
FT /note="ELK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00559"
FT DNA_BIND 263..326
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 11..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..119
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036180"
FT VAR_SEQ 120..126
FT /note="AYLDCQK -> MCRGGLQ (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036181"
FT CONFLICT 99
FT /note="A -> P (in Ref. 1; BAA03959)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 361 AA; 39898 MW; 2DDC03667D7716B3 CRC64;
MEEISHHFGV VGASGVHGGH QHQHHHHPWG SSLSAIVAPP PPPQLQQQQT QAGGMAHTPL
TLNTAAAAVG NPVLQLANGS LLDACGKAKE ASASASYAAD VEAIKAKIIS HPHYSSLLAA
YLDCQKVGAP PEVAARLTAV AQDLELRQRT ALGVLGAATE PELDQFMEAY HEMLVKYREE
LTRPLQEAME FLRRVETQLN TLSISGRSLR NILSSGSSEE DQEGSGGETE LPEIDAHGVD
QELKHHLLKK YSGYLSSLKQ ELSKKKKKGK LPKDARQQLL NWWELHYKWP YPSESQKVAL
AESTGLDLKQ INNWFINQRK RHWKPSDEMQ FVMMDGYHPT NAAAFYMDGH FINDGGLYRL
G
