KNOX3_HORVU
ID KNOX3_HORVU Reviewed; 364 AA.
AC Q43484; O22532;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Homeobox protein KNOX3;
DE AltName: Full=Hooded protein;
GN Name=KNOX3; Synonyms=K;
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. C1; TISSUE=Leaf;
RX PubMed=7715728; DOI=10.1038/374727a0;
RA Mueller K.J., Romano N., Gerstner O., Garcia-Maroto F., Pozzi C.,
RA Salamini F., Rohde W.;
RT "The barley hooded mutation caused by a duplication in a homeobox gene
RT intron.";
RL Nature 374:727-730(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND IDENTIFICATION OF DNA-BINDING SITE.
RC STRAIN=cv. Bomi; TISSUE=Seedling;
RX PubMed=9180261; DOI=10.1016/s0014-5793(97)00382-7;
RA Krusell L., Rasmussen I., Gausing K.;
RT "DNA binding sites recognised in vitro by a knotted class 1 homeodomain
RT protein encoded by the hooded gene, k, in barley (Hordeum vulgare).";
RL FEBS Lett. 408:25-29(1997).
CC -!- FUNCTION: May play a role in meristem formation and/or maintenance.
CC Overexpression causes the hooded phenotype characterized by the
CC appearance of an extra flower of inverse polarity on the lemma. Binds
CC to the DNA sequence 5'-TGAC-3'.
CC -!- SUBUNIT: Binds DNA as a monomer.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: The unit of inflorescence is the spikelet, which
CC bears a fertile tract, the lemma, and the floret consisting of palea,
CC two lodicules, three stamens and the pistil. The lemma is completed by
CC the awn, an appendage homologous to the laminae of normal leaves.
CC Expressed in the inflorescences and lemmas and at lower levels, in
CC palea and vascular bundles.
CC -!- DEVELOPMENTAL STAGE: Periclinal cell divisions in the subepidermal
CC layer of the awn primordium gives rise to a meristematic cushion in
CC hooded phenotypes. Expressed before the formation of the cushion and
CC continues as it is formed and in later stages its expression is down-
CC regulated.
CC -!- SIMILARITY: Belongs to the TALE/KNOX homeobox family.
CC {ECO:0000255|PROSITE-ProRule:PRU00559}.
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DR EMBL; X83518; CAA58503.1; -; Genomic_DNA.
DR EMBL; AF022390; AAB81079.1; -; mRNA.
DR PIR; S58871; S58871.
DR AlphaFoldDB; Q43484; -.
DR SMR; Q43484; -.
DR EnsemblPlants; HORVU.MOREX.r2.4HG0282910.1; HORVU.MOREX.r2.4HG0282910.1; HORVU.MOREX.r2.4HG0282910.
DR EnsemblPlants; HORVU.MOREX.r2.4HG0282910.1.mrna1; HORVU.MOREX.r2.4HG0282910.1.mrna1; HORVU.MOREX.r2.4HG0282910.1.
DR Gramene; HORVU.MOREX.r2.4HG0282910.1; HORVU.MOREX.r2.4HG0282910.1; HORVU.MOREX.r2.4HG0282910.
DR Gramene; HORVU.MOREX.r2.4HG0282910.1.mrna1; HORVU.MOREX.r2.4HG0282910.1.mrna1; HORVU.MOREX.r2.4HG0282910.1.
DR OMA; YHPTNAA; -.
DR ExpressionAtlas; Q43484; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IEA:EnsemblPlants.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:EnsemblPlants.
DR GO; GO:0009901; P:anther dehiscence; IEA:EnsemblPlants.
DR GO; GO:0010930; P:negative regulation of auxin mediated signaling pathway; IEA:EnsemblPlants.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:EnsemblPlants.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR005539; ELK_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR005540; KNOX1.
DR InterPro; IPR005541; KNOX2.
DR Pfam; PF03789; ELK; 1.
DR Pfam; PF05920; Homeobox_KN; 1.
DR Pfam; PF03790; KNOX1; 1.
DR Pfam; PF03791; KNOX2; 1.
DR SMART; SM01188; ELK; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM01255; KNOX1; 1.
DR SMART; SM01256; KNOX2; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51213; ELK; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus.
FT CHAIN 1..364
FT /note="Homeobox protein KNOX3"
FT /id="PRO_0000049165"
FT DOMAIN 246..266
FT /note="ELK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00559"
FT DNA_BIND 267..330
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 13..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..49
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 118
FT /note="Y -> H (in strain: cv. Bomi)"
FT VARIANT 210
FT /note="G -> V (in strain: cv. Bomi)"
SQ SEQUENCE 364 AA; 40344 MW; 60999A1B3AB3EAAA CRC64;
MEEIGHHFGL GATAHGQHHS QLPWGSSPLS AVISPPPQQQ QQHQQQSAGY LAHSPLSLNT
APPGVSHGGG SGCSNPVLQL ANGSLLEACA KAAKEPSSSS YAADVEAIKA KIISHPHYSS
LLAAYLDCQK VGAPPEVSAR LTAVAQDLEL RQRTALGGLG TATEPELDQF MEAYHEMLVK
YREELTRPLQ EAMEFLRRVE TQLNSLSISG RSLRNILSTG SSEEDQEGSG GETELPEIDA
HGVDQELKHH LLKKYSGYLS SLKQELSKKK KKGKLPKEAR QQLLSWWEMH YKWPYPSESQ
KVALAESTGL DLKQINNWFI NQRKRHWKPT DEMQFVMMDA YHPPNAAFYM DGHFVNDSGL
YRFG
