ID   KNU_ARATH               Reviewed;         161 AA.
AC   Q9FFX4;
DT   21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Zinc finger protein KNUCKLES;
GN   Name=KNU; OrderedLocusNames=At5g14010; ORFNames=MAC12.2;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS
RP   OF CYS-43.
RX   PubMed=15240552; DOI=10.1242/dev.01216;
RA   Payne T., Johnson S.D., Koltunow A.M.;
RT   "KNUCKLES (KNU) encodes a C2H2 zinc-finger protein that regulates
RT   development of basal pattern elements of the Arabidopsis gynoecium.";
RL   Development 131:3737-3749(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA   Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA   Miyajima N., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT   features of the 1.6 Mb regions covered by twenty physically assigned P1
RT   clones.";
RL   DNA Res. 4:215-230(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: May function as a transcriptional repressor of cellular
CC       proliferation that regulates floral determinacy and relative size of
CC       basal pattern elements along the proximo-distal axis of the developing
CC       gynoecium. {ECO:0000269|PubMed:15240552}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: First expressed in developing carpel primordia, and
CC       later in stamens and ovules of flower buds.
CC       {ECO:0000269|PubMed:15240552}.
CC   -!- DOMAIN: Contains a slightly degenerated ERF-associated amphiphilic
CC       repression (EAR) motif, which may be involved in the activity of
CC       transcriptional repression. {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AY612608; AAT27472.1; -; mRNA.
DR   EMBL; AB005230; BAB11129.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED91973.1; -; Genomic_DNA.
DR   RefSeq; NP_196905.1; NM_121404.2.
DR   AlphaFoldDB; Q9FFX4; -.
DR   STRING; 3702.AT5G14010.1; -.
DR   PaxDb; Q9FFX4; -.
DR   PRIDE; Q9FFX4; -.
DR   EnsemblPlants; AT5G14010.1; AT5G14010.1; AT5G14010.
DR   GeneID; 831249; -.
DR   Gramene; AT5G14010.1; AT5G14010.1; AT5G14010.
DR   KEGG; ath:AT5G14010; -.
DR   Araport; AT5G14010; -.
DR   TAIR; locus:2159088; AT5G14010.
DR   eggNOG; ENOG502S8MR; Eukaryota.
DR   HOGENOM; CLU_1646041_0_0_1; -.
DR   InParanoid; Q9FFX4; -.
DR   OMA; SHRLFPC; -.
DR   OrthoDB; 1561563at2759; -.
DR   PhylomeDB; Q9FFX4; -.
DR   PRO; PR:Q9FFX4; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FFX4; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0010582; P:floral meristem determinacy; IMP:TAIR.
DR   GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR   InterPro; IPR045320; JAGGED/KNUCKLES-like.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   PANTHER; PTHR45730; PTHR45730; 1.
DR   SUPFAM; SSF57667; SSF57667; 1.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; Differentiation; Flowering; Metal-binding; Nucleus;
KW   Reference proteome; Repressor; Transcription; Transcription regulation;
KW   Zinc; Zinc-finger.
FT   CHAIN           1..161
FT                   /note="Zinc finger protein KNUCKLES"
FT                   /id="PRO_0000047837"
FT   ZN_FING         38..60
FT                   /note="C2H2-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   REGION          1..33
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          142..161
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           155..159
FT                   /note="EAR-like (transcriptional repression)"
FT   COMPBIAS        18..33
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         43
FT                   /note="C->Y: In knu; induces flowers that are conditionally
FT                   male sterile and contains ectopic stamens and carpels that
FT                   originate from placental tissue within developing
FT                   gynoecia."
FT                   /evidence="ECO:0000269|PubMed:15240552"
SQ   SEQUENCE   161 AA;  18049 MW;  3E3BFF342D50A67E CRC64;
     MAEPPPSYLH FVGPAKTRSS SKRHSFSSSA HPASHRLFPC QYCPRKFYTS QALGGHQNAH
     KRERAAARRN LGVLANSPPI LDDNNTFLRP YPCFYQNPFQ GSTSGNEPLQ EQPTMMTMDG
     YDPFHPYPYV YPFALSGNNN DGGNGVMEED EPLDLDLSLR L