ARAJ_ECOLI
ID ARAJ_ECOLI Reviewed; 394 AA.
AC P23910; Q2MC30;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-1992, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Putative transporter AraJ;
GN Name=araJ; OrderedLocusNames=b0396, JW0386;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RX PubMed=1744033; DOI=10.1128/jb.173.24.7765-7771.1991;
RA Reeder T.C., Schleif R.F.;
RT "Mapping, sequence, and apparent lack of function of araJ, a gene of the
RT Escherichia coli arabinose regulon.";
RL J. Bacteriol. 173:7765-7771(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RA Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT "Sequence of minutes 4-25 of Escherichia coli.";
RL Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP INDUCTION.
RX PubMed=2231717; DOI=10.1016/s0022-2836(05)80163-9;
RA Hendrickson W., Stoner C., Schleif R.;
RT "Characterization of the Escherichia coli araFGH and araJ promoters.";
RL J. Mol. Biol. 215:497-510(1990).
RN [6]
RP SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: May be involved in either the transport or processing of
CC arabinose polymers. {ECO:0000305|PubMed:1744033}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:15919996}.
CC -!- INDUCTION: Induced by arabinose (PubMed:2231717) (Probable).
CC Transcription is dependent on the transcription factor AraC, the cAMP
CC receptor protein (CRP) and cAMP (PubMed:2231717).
CC {ECO:0000269|PubMed:2231717, ECO:0000305|PubMed:1744033}.
CC -!- DISRUPTION PHENOTYPE: No visible effect on arabinose uptake or growth
CC on arabinose. {ECO:0000269|PubMed:1744033}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB18120.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M64787; AAA23474.1; -; Genomic_DNA.
DR EMBL; U73857; AAB18120.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC73499.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76176.1; -; Genomic_DNA.
DR PIR; B43750; B43750.
DR RefSeq; NP_414930.3; NC_000913.3.
DR RefSeq; WP_001326926.1; NZ_SSZK01000009.1.
DR AlphaFoldDB; P23910; -.
DR SMR; P23910; -.
DR BioGRID; 4263172; 19.
DR STRING; 511145.b0396; -.
DR TCDB; 2.A.1.2.14; the major facilitator superfamily (mfs).
DR PaxDb; P23910; -.
DR PRIDE; P23910; -.
DR EnsemblBacteria; AAC73499; AAC73499; b0396.
DR EnsemblBacteria; BAE76176; BAE76176; BAE76176.
DR GeneID; 949077; -.
DR KEGG; ecj:JW0386; -.
DR KEGG; eco:b0396; -.
DR PATRIC; fig|511145.12.peg.409; -.
DR EchoBASE; EB0058; -.
DR eggNOG; COG2814; Bacteria.
DR HOGENOM; CLU_001265_61_2_6; -.
DR InParanoid; P23910; -.
DR OMA; GWMITIY; -.
DR PhylomeDB; P23910; -.
DR BioCyc; EcoCyc:ARAJ-MON; -.
DR PRO; PR:P23910; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..394
FT /note="Putative transporter AraJ"
FT /id="PRO_0000084832"
FT TOPO_DOM 1..4
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 5..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..41
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..69
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..93
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 117..128
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..155
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 179..198
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 199..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 222..235
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 256..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..293
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 294..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 317..322
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 323..342
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..351
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 375..394
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255, ECO:0000269|PubMed:15919996"
SQ SEQUENCE 394 AA; 41926 MW; 40C1B0F1451A817A CRC64;
MKKVILSLAL GTFGLGMAEF GIMGVLTELA HNVGISIPAA GHMISYYALG VVVGAPIIAL
FSSRYSLKHI LLFLVALCVI GNAMFTLSSS YLMLAIGRLV SGFPHGAFFG VGAIVLSKII
KPGKVTAAVA GMVSGMTVAN LLGIPLGTYL SQEFSWRYTF LLIAVFNIAV MASVYFWVPD
IRDEAKGNLR EQFHFLRSPA PWLIFAATMF GNAGVFAWFS YVKPYMMFIS GFSETAMTFI
MMLVGLGMVL GNMLSGRISG RYSPLRIAAV TDFIIVLALL MLFFCGGMKT TSLIFAFICC
AGLFALSAPL QILLLQNAKG GELLGAAGGQ IAFNLGSAVG AYCGGMMLTL GLAYNYVALP
AALLSFAAMS SLLLYGRYKR QQAADTPVLA KPLG
