KOI_DROME
ID KOI_DROME Reviewed; 965 AA.
AC A0A0B4KEE4;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-2015, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Klaroid protein {ECO:0000305};
GN Name=koi {ECO:0000312|FlyBase:FBgn0265003};
GN ORFNames=CG44154 {ECO:0000312|FlyBase:FBgn0265003};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND
RP FUNCTION.
RX PubMed=18820457; DOI=10.4161/fly.4254;
RA Kracklauer M.P., Banks S.M., Xie X., Wu Y., Fischer J.A.;
RT "Drosophila klaroid encodes a SUN domain protein required for Klarsicht
RT localization to the nuclear envelope and nuclear migration in the eye.";
RL Fly 1:75-85(2007).
CC -!- FUNCTION: Component of the LINC (LInker of Nucleoskeleton and
CC Cytoskeleton) complex involved in the connection between the nuclear
CC lamina and the cytoskeleton (By similarity). Is required to nuclear
CC migration in eye and to anchor klar in the nuclear membrane
CC (PubMed:18820457). {ECO:0000250|UniProtKB:O94901,
CC ECO:0000269|PubMed:18820457}.
CC -!- SUBUNIT: Core component of the LINC complex which is composed of inner
CC nuclear membrane SUN domain-containing proteins coupled to outer
CC nuclear membrane KASH domain-containing nesprins.
CC {ECO:0000250|UniProtKB:O94901}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}. Cytoplasm, perinuclear region
CC {ECO:0000269|PubMed:18820457}. Note=Lam is required for perinuclear
CC localization of Koi. {ECO:0000269|PubMed:18820457}.
CC -!- TISSUE SPECIFICITY: Expressed in all cells in the eye disk.
CC {ECO:0000269|PubMed:18820457}.
CC -!- DISRUPTION PHENOTYPE: Homozygous mutant flies live to adulthood and are
CC fertile and exhibit rough and irregular eyes. Flies have an aberrant R-
CC cell rhabdomeres morphology and R-cell and cone cell nuclei are
CC mispositioned. {ECO:0000269|PubMed:18820457}.
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DR EMBL; AE013599; AGB93271.1; -; Genomic_DNA.
DR EMBL; AE013599; AGB93272.1; -; Genomic_DNA.
DR RefSeq; NP_001260738.1; NM_001273809.1.
DR RefSeq; NP_001260739.1; NM_001273810.1.
DR AlphaFoldDB; A0A0B4KEE4; -.
DR SMR; A0A0B4KEE4; -.
DR STRING; 7227.FBpp0292403; -.
DR EnsemblMetazoa; FBtr0335512; FBpp0307480; FBgn0265003.
DR EnsemblMetazoa; FBtr0335514; FBpp0307482; FBgn0265003.
DR GeneID; 35594; -.
DR KEGG; dme:Dmel_CG44154; -.
DR CTD; 35594; -.
DR FlyBase; FBgn0265003; koi.
DR VEuPathDB; VectorBase:FBgn0265003; -.
DR OrthoDB; 1569602at2759; -.
DR BioGRID-ORCS; 35594; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35594; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0265003; Expressed in egg cell and 43 other tissues.
DR ExpressionAtlas; A0A0B4KEE4; baseline and differential.
DR GO; GO:0005639; C:integral component of nuclear inner membrane; IEA:InterPro.
DR GO; GO:0034993; C:meiotic nuclear membrane microtubule tethering complex; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; IDA:FlyBase.
DR GO; GO:0034399; C:nuclear periphery; IDA:FlyBase.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:FlyBase.
DR GO; GO:0043495; F:protein-membrane adaptor activity; IBA:GO_Central.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IMP:FlyBase.
DR GO; GO:0006998; P:nuclear envelope organization; IBA:GO_Central.
DR GO; GO:0007097; P:nuclear migration; IMP:FlyBase.
DR GO; GO:0051647; P:nucleus localization; IMP:FlyBase.
DR InterPro; IPR045119; SUN1-5.
DR InterPro; IPR012919; SUN_dom.
DR PANTHER; PTHR12911; PTHR12911; 1.
DR Pfam; PF07738; Sad1_UNC; 1.
DR PROSITE; PS51469; SUN; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Cytoplasm; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..965
FT /note="Klaroid protein"
FT /id="PRO_0000454909"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 801..963
FT /note="SUN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00802"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 585..612
FT /evidence="ECO:0000255"
SQ SEQUENCE 965 AA; 109725 MW; D695795D781BEA85 CRC64;
MSENTYQIET RRRSRSKTPF LRSSCDHENC EHAGEEGHVH HLKRKSAAPN VQTIIEEHIV
ESSISKKTRA KAFAQLTSDY SSDDMTPDAK RKQNSITATV TSILTKRSGG ATSTPRNRSQ
LETTQNTLNS AQEKLNQSNG NLSSGNVSDY LAYIEYRDAG EYWNKTPKTD YTYSELSPHR
RQLAPGIVAM PNMSRKSLEN HNDRVNYMVQ QNPAQEEFIR RRYQSKYTQQ VNYDSADELD
ATFGQQKQSW WLIRLIQLVV SSITTVWSRV TNLSATETTA YQNYHAKRQQ SQQVGLWWKI
VQTIGGGLAS LLRYLYVFIG SVLSLDTWLL RSSDAENKSK KRFLIFLLIL LPLLLLSGWL
LLQEDQRSAY VQRAEALLPL PLSIFGSLRS RFSNAGATLK SWMEVPTVRS PQREAEAIKV
NMASIEQNIQ KALTAEEYEN ILNHVNSYVQ QLVELKMQQH SKELAPQQIE LFVKLMKENL
KQIMYKTELS EKDLSDLAIK LKLELQSSGG WQDGAKLSQA NLEEITKLIK AEVHLHESHY
TIQLDRIDFA SLLERILAAP ALADFVDARI SLRVGELEPK ESSGSSDAEV QIERLNREIA
FIKLALSDKQ AENADLHQSI SNLKLGQEDL LERIQQHELS QDRRFHGLLA EIENKLSALN
DSQFALLNKQ IKLSLVEILG FKQSTAGGSA GQLDDFDLQT WVRSMFVAKD YLEQQLLELN
KRTNNNIRDE IERSSILLMS DISQRLKREI LLVVEAKHNE STKALKGHIR EEEVRQIVKT
VLAIYDADKT GLVDFALESA GGQILSTRCT ESYQTKSAQI SVFGIPLWYP TNTPRVAISP
NVQPGECWAF QGFPGFLVLK LNSLVYVTGF TLEHIPKSLS PTGRIESAPR NFTVWGLEQE
KDQEPVLFGD YQFEDNGASL QYFAVQNLDI KRPYEIVELR IETNHGHPTY TCLYRFRVHG
KPPAT
