ID   KOJR_ASPOR              Reviewed;         555 AA.
AC   Q2U5H9;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Transcription factor kojR {ECO:0000305};
DE   AltName: Full=Kojic acid cluster biosynthesis regulator {ECO:0000305};
GN   Name=kojR {ECO:0000303|PubMed:21514215}; ORFNames=AO090113000137;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=7714722; DOI=10.1111/j.2042-7158.1994.tb03253.x;
RA   Cabanes J., Chazarra S., Garcia-Carmona F.;
RT   "Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor
RT   of catecholase activity of tyrosinase.";
RL   J. Pharm. Pharmacol. 46:982-985(1994).
RN   [3]
RP   REVIEW ON BIOTECHNOLOGY.
RX   PubMed=17119644; DOI=10.1039/b603758p;
RA   Bentley R.;
RT   "From miso, sake and shoyu to cosmetics: a century of science for kojic
RT   acid.";
RL   Nat. Prod. Rep. 23:1046-1062(2006).
RN   [4]
RP   INDUCTION.
RX   PubMed=21897021; DOI=10.1271/bbb.110235;
RA   Oda K., Kobayashi A., Ohashi S., Sano M.;
RT   "Aspergillus oryzae laeA regulates kojic acid synthesis genes.";
RL   Biosci. Biotechnol. Biochem. 75:1832-1834(2011).
RN   [5]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=21514215; DOI=10.1016/j.jbiosc.2011.03.010;
RA   Marui J., Yamane N., Ohashi-Kunihiro S., Ando T., Terabayashi Y., Sano M.,
RA   Ohashi S., Ohshima E., Tachibana K., Higa Y., Nishimura M., Koike H.,
RA   Machida M.;
RT   "Kojic acid biosynthesis in Aspergillus oryzae is regulated by a
RT   Zn(II)(2)Cys(6) transcriptional activator and induced by kojic acid at the
RT   transcriptional level.";
RL   J. Biosci. Bioeng. 112:40-43(2011).
RN   [6]
RP   BIOTECHNOLOGY.
RX   PubMed=27197670; DOI=10.4014/jmb.1603.03040;
RA   Kim T.Y., Jang J.Y., Jeon S.J., Lee H.W., Bae C.H., Yeo J.H., Lee H.B.,
RA   Kim I.S., Park H.W., Kim J.C.;
RT   "Nematicidal activity of kojic acid produced by Aspergillus oryzae against
RT   Meloidogyne incognita.";
RL   J. Microbiol. Biotechnol. 26:1383-1391(2016).
CC   -!- FUNCTION: Transcription factor that regulates the gene cluster that
CC       mediates the biosynthesis of 5-hydroxy-2-hydroxymethyl-1,4-pyrone, also
CC       know as kojic acid, a by-product in the fermentation process of malting
CC       rice that acts as a chelation agent (PubMed:21897021).
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- INDUCTION: Expression is positively regulated by the secondary
CC       metabolism general regulator laeA (PubMed:21897021).
CC       {ECO:0000269|PubMed:21897021}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the transcription of kojA and kojT, and
CC       blocks the production of kojic acis (PubMed:21514215).
CC       {ECO:0000269|PubMed:21514215}.
CC   -!- BIOTECHNOLOGY: Kojic acid can be used for several biotechnological
CC       applications, including use as an antibiotic, as an additive to prevent
CC       browning of food materials, and as an antioxidant (PubMed:17119644).
CC       Kojic acid is also interesting as an inhibitor of tyrosinase
CC       (PubMed:7714722). Finally, kojic acid has also been shown to have
CC       strong nematicidal activity (PubMed:27197670).
CC       {ECO:0000269|PubMed:17119644, ECO:0000269|PubMed:27197670,
CC       ECO:0000269|PubMed:7714722}.
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DR   EMBL; AP007166; BAE63186.1; -; Genomic_DNA.
DR   RefSeq; XP_001824319.1; XM_001824267.1.
DR   AlphaFoldDB; Q2U5H9; -.
DR   SMR; Q2U5H9; -.
DR   PRIDE; Q2U5H9; -.
DR   EnsemblFungi; BAE63186; BAE63186; AO090113000137.
DR   GeneID; 5995966; -.
DR   KEGG; aor:AO090113000137; -.
DR   VEuPathDB; FungiDB:AO090113000137; -.
DR   HOGENOM; CLU_019691_1_0_1; -.
DR   OMA; WISYEYG; -.
DR   Proteomes; UP000006564; Chromosome 5.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:2001317; P:kojic acid biosynthetic process; IMP:AspGD.
DR   GO; GO:1900396; P:positive regulation of kojic acid biosynthetic process; IMP:AspGD.
DR   GO; GO:1900378; P:positive regulation of secondary metabolite biosynthetic process; IMP:AspGD.
DR   GO; GO:0044550; P:secondary metabolite biosynthetic process; IMP:AspGD.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00906; Fungal_trans; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..555
FT                   /note="Transcription factor kojR"
FT                   /id="PRO_0000436875"
FT   DNA_BIND        21..47
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          51..73
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   555 AA;  62861 MW;  AADA908734280D59 CRC64;
     MSLNTDDSGR IRTRQRAKRA CETCKLRKRK CDGHEPCTYC LRYEYQCTFK PHPRRKPAAS
     KSSARPSEEE DSPKFLDRVD ANQEHMEANS GTAFPHLLGM RLNPQGAPKV YGFSWNLGPR
     DEPLEPFTNL TDLISREEME DLASHYLKKI HPVYAVLDPD TLRQKIVARW HDPATAASYD
     PILCGVAALG SLYSGHQEHP KEGALVQSAK EMLETTRISK TTLLHHATAW ILRTIYLRST
     NCPHASWMAS CSTMHIIEAI GAHQDPELVS LVYSDTADVS VNDESQRRLF WVATVLNSWI
     SYEYGRSRVI LRGVSCKPPL PRTGDFTTDL ISMYQISERL DPDQNNKLSD LEDALSRVER
     LTLSHDALIL SQSNLALTIY RRLRVASSNI SNDILTRIIR LGNDGLEAAV RLAEDRSPWW
     HVANIPFQFL CILLAIDTRE SLSYVGPALR SFRAITRHYS TPTLHTALET IESLVRLSQN
     KKERDLTLLR DSMQQEDPGL TEQGSTTSQA FNDASWLGAT GDLTLPDNFD WDWNVFLDTQ
     VPFFDEGEAG GQRYR