KOJT_ASPOR
ID KOJT_ASPOR Reviewed; 564 AA.
AC Q2U5H8;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Probable efflux pump kojT {ECO:0000305};
DE AltName: Full=Kojic acid transporter {ECO:0000303|PubMed:21514215};
GN Name=kojT {ECO:0000303|PubMed:21514215}; ORFNames=AO090113000138;
OS Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=510516;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 42149 / RIB 40;
RX PubMed=16372010; DOI=10.1038/nature04300;
RA Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA Kikuchi H.;
RT "Genome sequencing and analysis of Aspergillus oryzae.";
RL Nature 438:1157-1161(2005).
RN [2]
RP BIOTECHNOLOGY.
RX PubMed=7714722; DOI=10.1111/j.2042-7158.1994.tb03253.x;
RA Cabanes J., Chazarra S., Garcia-Carmona F.;
RT "Kojic acid, a cosmetic skin whitening agent, is a slow-binding inhibitor
RT of catecholase activity of tyrosinase.";
RL J. Pharm. Pharmacol. 46:982-985(1994).
RN [3]
RP REVIEW ON BIOTECHNOLOGY.
RX PubMed=17119644; DOI=10.1039/b603758p;
RA Bentley R.;
RT "From miso, sake and shoyu to cosmetics: a century of science for kojic
RT acid.";
RL Nat. Prod. Rep. 23:1046-1062(2006).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20849972; DOI=10.1016/j.fgb.2010.08.014;
RA Terabayashi Y., Sano M., Yamane N., Marui J., Tamano K., Sagara J.,
RA Dohmoto M., Oda K., Ohshima E., Tachibana K., Higa Y., Ohashi S., Koike H.,
RA Machida M.;
RT "Identification and characterization of genes responsible for biosynthesis
RT of kojic acid, an industrially important compound from Aspergillus
RT oryzae.";
RL Fungal Genet. Biol. 47:953-961(2010).
RN [5]
RP INDUCTION.
RX PubMed=21897021; DOI=10.1271/bbb.110235;
RA Oda K., Kobayashi A., Ohashi S., Sano M.;
RT "Aspergillus oryzae laeA regulates kojic acid synthesis genes.";
RL Biosci. Biotechnol. Biochem. 75:1832-1834(2011).
RN [6]
RP INDUCTION.
RX PubMed=21514215; DOI=10.1016/j.jbiosc.2011.03.010;
RA Marui J., Yamane N., Ohashi-Kunihiro S., Ando T., Terabayashi Y., Sano M.,
RA Ohashi S., Ohshima E., Tachibana K., Higa Y., Nishimura M., Koike H.,
RA Machida M.;
RT "Kojic acid biosynthesis in Aspergillus oryzae is regulated by a
RT Zn(II)(2)Cys(6) transcriptional activator and induced by kojic acid at the
RT transcriptional level.";
RL J. Biosci. Bioeng. 112:40-43(2011).
RN [7]
RP INDUCTION.
RX PubMed=26657710; DOI=10.1016/j.jbiotec.2015.11.023;
RA Liu J.M., Yu T.C., Lin S.P., Hsu R.J., Hsu K.D., Cheng K.C.;
RT "Evaluation of kojic acid production in a repeated-batch PCS biofilm
RT reactor.";
RL J. Biotechnol. 218:41-48(2016).
RN [8]
RP BIOTECHNOLOGY.
RX PubMed=27197670; DOI=10.4014/jmb.1603.03040;
RA Kim T.Y., Jang J.Y., Jeon S.J., Lee H.W., Bae C.H., Yeo J.H., Lee H.B.,
RA Kim I.S., Park H.W., Kim J.C.;
RT "Nematicidal activity of kojic acid produced by Aspergillus oryzae against
RT Meloidogyne incognita.";
RL J. Microbiol. Biotechnol. 26:1383-1391(2016).
CC -!- FUNCTION: Probable efflux pump; part of the gene cluster that mediates
CC the biosynthesis of 5-hydroxy-2-hydroxymethyl-1,4-pyrone, also know as
CC kojic acid, a by-product in the fermentation process of malting rice
CC that acts as a chelation agent (PubMed:20849972). Glucose might be
CC converted to kojic acid by a combination of dehydrogenase and
CC dehydratase reactions involving kojA and probably additional enzymes
CC (PubMed:20849972). {ECO:0000269|PubMed:20849972}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Expression is controlled by the kojic acid gene cluster
CC transcription factor kojR (PubMed:21514215). Expression is also
CC positively regulated by the secondary metabolism general regulator laeA
CC (PubMed:21897021). Finally, nitrogen deficiency also positively
CC regulates expression (PubMed:26657710). {ECO:0000269|PubMed:21514215,
CC ECO:0000269|PubMed:21897021, ECO:0000269|PubMed:26657710}.
CC -!- DISRUPTION PHENOTYPE: Leads to the loss of kojic acid production
CC (PubMed:20849972). {ECO:0000269|PubMed:20849972}.
CC -!- BIOTECHNOLOGY: Kojic acid can be used for several biotechnological
CC applications, including use as an antibiotic, as an additive to prevent
CC browning of food materials, and as an antioxidant (PubMed:17119644).
CC Kojic acid is also interesting as an inhibitor of tyrosinase
CC (PubMed:7714722). Finally, kojic acid has also been shown to have
CC strong nematicidal activity (PubMed:27197670).
CC {ECO:0000269|PubMed:17119644, ECO:0000269|PubMed:27197670,
CC ECO:0000269|PubMed:7714722}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; AP007166; BAE63187.1; -; Genomic_DNA.
DR RefSeq; XP_001824320.1; XM_001824268.1.
DR AlphaFoldDB; Q2U5H8; -.
DR PRIDE; Q2U5H8; -.
DR EnsemblFungi; BAE63187; BAE63187; AO090113000138.
DR GeneID; 5995967; -.
DR KEGG; aor:AO090113000138; -.
DR VEuPathDB; FungiDB:AO090113000138; -.
DR HOGENOM; CLU_008455_11_1_1; -.
DR OMA; QLAFRFI; -.
DR Proteomes; UP000006564; Chromosome 5.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:2001317; P:kojic acid biosynthetic process; IMP:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..564
FT /note="Probable efflux pump kojT"
FT /id="PRO_0000436874"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 278..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 437..457
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 462..482
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 500..520
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 530..550
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 113
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 564 AA; 62915 MW; 7D76C2493A1E25EA CRC64;
MQSFRQYRRM RRDLQESIKL HGPYAAAGDR HVQPTDDILE DADDARLEKG IHSMNGHGQS
PYSTPGIVLH DGQRVTVPGV NLRRASEICE RVNTKTLFIV GFDGPDDQLN PKNWSIGRKW
ATLGIVGTTG MLVGWASSID STVIKQGQEA FGVSEVAESL ATALFLFAFG FGSLVAAPFS
ETVGRNPVYI ATLSILMIFT MASGLAPNFG AQLAFRFLAG LFGCTPMTTF GGSMADIFDP
MDRTYAFPVC CTLSFLGPFL APMVGAFIGQ STHISWRWTE WCTLIMAALV TGAIFLFVPE
TYGPVLLQWK AKQLREITGD PRFMAEIELR QTSLVTRLMH SCSRPFHLFF REIMVALFTM
YLVVVYIVLF GFLTGYEFIF GRTYGFTQGS VGLTFIGMNI GFLIAFAMVP HIYFSYKKRL
QNAIENGHNG LPPEERLWFA MYGAPWLPIS LFWMGWTSYP SISYWSPLVA SVAFGFSVQG
IFISTYQYLI DTYELFAASA LVSATFFRYI AAGAMVIVSI PMYGNLGVHW SLTLLGCISV
LMTPVPYIFY KYGHVIRQRN KKTP
