ID   KORA2_ECOLX             Reviewed;         101 AA.
AC   P03052;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=TrfB transcriptional repressor protein;
DE   AltName: Full=Regulatory protein KorA;
GN   Name=trfB; Synonyms=korA;
OS   Escherichia coli.
OG   Plasmid IncP-alpha RK2.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6316262; DOI=10.1093/nar/11.21.7453;
RA   Bechhofer D.H., Figurski D.H.;
RT   "Map location and nucleotide sequence of korA, a key regulatory gene of
RT   promiscuous plasmid RK2.";
RL   Nucleic Acids Res. 11:7453-7469(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3520485; DOI=10.1093/nar/14.11.4453;
RA   Thomas C.M., Smith C.A.;
RT   "The trfB region of broad host range plasmid RK2: the nucleotide sequence
RT   reveals incC and key regulatory gene trfB/korA/korD as overlapping genes.";
RL   Nucleic Acids Res. 14:4453-4469(1986).
CC   -!- FUNCTION: In conjunction with KorB, inhibits the transcription of kilA,
CC       trfA and korAB operons. In conjunction with KorC is responsible for the
CC       negative control of kilC and kilE operons.
CC   -!- INTERACTION:
CC       P03052; P07674: korB; NbExp=2; IntAct=EBI-6405477, EBI-6405482;
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DR   EMBL; X03962; CAA27596.1; -; Genomic_DNA.
DR   RefSeq; WP_011205830.1; NZ_NJTR01000055.1.
DR   PDB; 2N5G; NMR; -; A/B=1-101.
DR   PDB; 2W7N; X-ray; 1.85 A; A/B=1-101.
DR   PDB; 5CKT; X-ray; 2.00 A; A/B/C=1-99, D=1-101.
DR   PDB; 5CLV; X-ray; 2.50 A; A/B=2-97, E/F/I/J/M/N=2-66.
DR   PDB; 5CM3; X-ray; 2.30 A; A/B=1-97.
DR   PDBsum; 2N5G; -.
DR   PDBsum; 2W7N; -.
DR   PDBsum; 5CKT; -.
DR   PDBsum; 5CLV; -.
DR   PDBsum; 5CM3; -.
DR   AlphaFoldDB; P03052; -.
DR   BMRB; P03052; -.
DR   SMR; P03052; -.
DR   DIP; DIP-27644N; -.
DR   IntAct; P03052; 1.
DR   EvolutionaryTrace; P03052; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR032428; TrfB.
DR   Pfam; PF16509; KORA; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Plasmid; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..101
FT                   /note="TrfB transcriptional repressor protein"
FT                   /id="PRO_0000068380"
FT   DNA_BIND        37..56
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   HELIX           7..14
FT                   /evidence="ECO:0007829|PDB:2W7N"
FT   HELIX           21..31
FT                   /evidence="ECO:0007829|PDB:2W7N"
FT   HELIX           37..44
FT                   /evidence="ECO:0007829|PDB:2W7N"
FT   HELIX           48..64
FT                   /evidence="ECO:0007829|PDB:2W7N"
FT   STRAND          65..67
FT                   /evidence="ECO:0007829|PDB:5CLV"
FT   STRAND          71..78
FT                   /evidence="ECO:0007829|PDB:2W7N"
FT   HELIX           80..94
FT                   /evidence="ECO:0007829|PDB:2W7N"
SQ   SEQUENCE   101 AA;  11306 MW;  25622FB8F98F21A5 CRC64;
     MKKRLTESQF QEAIQGLEVG QQTIEIARGV LVDGKPQATF ATSLGLTRGA VSQAVHRVWA
     AFEDKNLPEG YARVTAVLPE HQAYIVRKWE ADAKKKQETK R