ID   ARALA_ARAEL             Reviewed;          25 AA.
AC   P83572;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Aralin A chain;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Fragment;
OS   Aralia elata (Japanese angelica tree).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Araliaceae; Aralia.
OX   NCBI_TaxID=82095 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBUNIT, AND GLYCOSYLATION.
RC   TISSUE=Shoot {ECO:0000269|PubMed:12963119};
RX   PubMed=12963119; DOI=10.1016/s0304-3835(03)00348-3;
RA   Tomatsu M., Ohnishi-Kameyama M., Shibamoto N.;
RT   "Aralin, a new cytotoxic protein from Aralia elata, inducing apoptosis in
RT   human cancer cells.";
RL   Cancer Lett. 199:19-25(2003).
RN   [2]
RP   PROTEIN SEQUENCE OF N-TERMINUS, AND CHARACTERIZATION.
RX   PubMed=15124096; DOI=10.1055/s-2004-818979;
RA   Tomatsu M., Mujin T., Shibamoto N., Tashiro F., Ikuta A.;
RT   "Production of aralin, a selective cytotoxic lectin against human
RT   transformed cells, in callus culture of Aralia elata.";
RL   Planta Med. 70:469-471(2004).
RN   [3]
RP   FUNCTION.
RX   PubMed=15493877; DOI=10.1515/bc.2004.107;
RA   Tomatsu M., Kondo T., Yoshikawa T., Komeno T., Adachi N., Kawasaki Y.,
RA   Ikuta A., Tashiro F.;
RT   "An apoptotic inducer, aralin, is a novel type II ribosome-inactivating
RT   protein from Aralia elata.";
RL   Biol. Chem. 385:819-827(2004).
CC   -!- FUNCTION: Toxic type II ribosome-inactivating protein (RIP). Induces
CC       apoptosis. Has cytotoxic activity against several human cancer cell
CC       lines. Is less cytotoxic to normal human cells.
CC       {ECO:0000269|PubMed:12963119, ECO:0000269|PubMed:15493877}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SUBUNIT: Disulfide-linked dimer of A and B chains.
CC       {ECO:0000269|PubMed:12963119}.
CC   -!- PTM: Glycosylated. {ECO:0000269|PubMed:12963119}.
CC   -!- MISCELLANEOUS: The inhibitory concentration 50% (IC50) is 0.8 ng/ml for
CC       transformed cells and 10 ng/ml for normal cells.
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 2
CC       RIP subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P83572; -.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0044533; P:positive regulation of apoptotic process in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Apoptosis; Direct protein sequencing; Disulfide bond; Glycoprotein;
KW   Hydrolase; Toxin.
FT   CHAIN           1..>25
FT                   /note="Aralin A chain"
FT                   /id="PRO_0000221396"
FT   NON_TER         25
FT                   /evidence="ECO:0000303|PubMed:12963119"
SQ   SEQUENCE   25 AA;  2734 MW;  857D34B58BFDB672 CRC64;
     ANFPTVSLNT VGITRQIPQD FMNAV